| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 96.0% | 67.1 / ‑5.4 | 26 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base C (10/14); minor base T (0/1); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAC > NC_000913/2132647‑2132920 | ggATCTGGGAGGGGGTGAACAGCTGATAGTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGATGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTc < 1:156723/143‑1 (MQ=255) aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGGCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGGGAGCTGCACTTCACCGAGGTTGATGTGCAGGCTCTg > 2:283301/1‑143 (MQ=255) ggcgaccAGCTGATATGTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt < 1:48406/137‑1 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTCATATGCAGGATATGAATCACCtctt > 2:106008/1‑143 (MQ=255) gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc < 1:278848/143‑1 (MQ=255) gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc > 2:278848/1‑143 (MQ=255) ctaacgaGCTGATAATTTGTGCTGGCGCGGTCGCATTTGTATTTGTGCTTGCGGTAATAGTGGAAGTACGCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc < 1:151013/137‑1 (MQ=255) tcTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc < 2:294190/142‑1 (MQ=255) atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCg > 2:706175/1‑143 (MQ=255) ttacgtgcgcgcTCGAAGTGTTCTTTGTGCTTGCTGTAAATGGGGAAGTAACCTTAGAATTTTTTTGGTGACGAGGTGATCTGCATTTTACCGTGATTGATATGCAGGCTCTGTGTCGCCTCTTCTACTTTCCACGGTTCGcc < 2:308691/137‑1 (MQ=255) gtccattttGTCTTTGTTCTTGCCGTAAAACTGCAAGTACCCGGAGAATTTTTTCGGTGACGCGGTGATCTGTATCTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca < 2:391349/137‑1 (MQ=255) cGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc < 2:469587/143‑1 (MQ=255) gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCCCTTGTACTTTCAACGGTTCGCCGTACTccccccccg > 1:336525/1‑142 (MQ=255) tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGc < 1:618389/143‑1 (MQ=255) ttGCGGTAAAAGTGGAAGTTCCCGGAGTATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc < 1:376907/143‑1 (MQ=255) aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGGTCTGCATTTCACCGTGATTGATATGTAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTCCGCCGTACGCCACCACCCTCCCCAGGAAGACGTCGTAATCAtgt > 2:77958/1‑143 (MQ=255) tgggtgTACGCGGAGATTTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGTGTTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCGACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGc < 2:632622/138‑1 (MQ=255) cccGGAGACTTTTTTCCGTGACGACGTGATCTGCATTTCACCGTGATTGATATCCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa < 1:408842/143‑1 (MQ=255) ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAGTTTCCACGGTTCGCCGTACTCCACCACCATCCGCTGGAAGATGTCGTACTGCTGTGACGCGTTCAGTAAGGTaa > 1:7557/1‑142 (MQ=255) tttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGGCGCCTCTTCTACTTTCTCCGGTTCGCCGTACTCCACGCCCATCCGCAGACCGATGTCGGAATCCTGTGCCGCTTCCAGTCCGGTATCGa > 2:110039/1‑143 (MQ=255) tCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAATCCACCACCATCCGCAGAAAGACCGATGAAACCTGTGCCGCTTTCAGTTTGGTATCGcac > 2:342315/1‑143 (MQ=255) caTCTGCATTTCTCCGTTATTGATATGCATGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCTCCGTACGCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa < 1:204284/142‑1 (MQ=255) tCTGCTTTTCACCGTGATTGATATGCAGGTTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa < 2:18616/143‑1 (MQ=255) ttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTGCACCACCATCCGCCGGAAGATGTCGTAATCCTGTGCCGCTTTCCGTTCGGGATCGAACACACACACGTTGAAAAGCCATg > 2:149729/1‑143 (MQ=255) tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTCCGGTATCGAACAAACACACTTTGAAACGCCATGCCCAGGt > 2:740864/1‑143 (MQ=255) cggtGCGGGCTCTGGGGCGGCTCTTCTACTTTCCACGGTGCGCCGTGCGCCCGCGCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc < 2:593798/140‑1 (MQ=255) | GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAC > NC_000913/2132647‑2132920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |