Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,661,830 C→T G101D (GGC→GAC)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,8300CT97.5% 100.6 / NA 40G101D (GGC→GAC) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (25/14);  minor base G (1/0);  total (26/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAA  >  NC_000913/2661694‑2661967
                                                                                                                                        |                                                                                                                                         
gtcggGAGTGCGCTGACGACCAGACACATCCAGCACTGCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCt                                                                                                                                     <  1:706926/143‑1 (MQ=255)
 tcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTCCAGTCATGCTGTTGACACCACCGCCGAGCGAGTGGCTTGAAGCACGCCCCAGCGCGTGGGTCAGGCATTTATCGGCGCCCTg                                                                                                                                    >  1:176758/1‑143 (MQ=255)
  cgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAACTCACGCCACCGCGCGTGGGTCAGGCATTTATCGTCGCCCTgg                                                                                                                                   <  1:472434/143‑1 (MQ=255)
           gCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGGCt                                                                                                                          >  1:526908/1‑143 (MQ=255)
             tGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAAAGTTGTTGAGAAAAACGGTGAGACGGTCGCTAAAATCACGCTACAGCGCGTGGGGCAGGCATTTATCGTCGACCTGGCAGCCGCCtct                                                                                                                        >  2:92349/1‑141 (MQ=255)
                 gacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACATCGCGTGAGTCAGGCATTTATCGTCTACCTGGCAGCCGCCTTTAccc                                                                                                                    >  1:297566/1‑143 (MQ=255)
                   ccAGACACATCCAGCACTTCCTGGTTATCAGCCAGTTGGCGTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGCGGGTCAGGCATTTATCGTCGCCCCGGCAGCCGCCCTTAcccgg                                                                                                                  >  2:642112/1‑141 (MQ=255)
                         acaTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCCGGCAGTTCTCGTAGCCCTGCCAGCCGCCTTTACGCTGACCACg                                                                                                            >  1:47860/1‑143 (MQ=255)
                           aTCCAGCACTTCCTGGTAATTAAGGAGTGCGACGAAAGAAATGTTGTTGAGAAAACCGATGAGACGGTGGCTCGAATCACGCGACAGCGCGTGGGTCAGGCAGTTATGGTCGCACTGGCAGCGGCGTGTACCGTGACGACggg                                                                                                          >  2:389185/1‑143 (MQ=255)
                            tCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGt                                                                                                         <  1:136338/143‑1 (MQ=255)
                                     tCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTAc                                                                                                >  1:572840/1‑143 (MQ=255)
                                              ttaACCAGTTCGCCTAAAGGAAGGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTc                                                                                       <  1:235318/143‑1 (MQ=255)
                                                aaCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCaa                                                                                     >  1:731884/1‑143 (MQ=255)
                                                 aCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCTTCAAc                                                                                    >  1:627151/1‑143 (MQ=255)
                                                 aCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGCTGGCATCTTCAGATCCGTCAAc                                                                                    >  1:53649/1‑143 (MQ=255)
                                                     gTTCGCCTAACGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGCGTCAGACATTTATCGTCGCCCTGGCAGCCGCCTTTGCCCTCACCACGGGTCGCGTCTCCGCCTTCGGCGACGgcg                                                                                >  1:709116/1‑143 (MQ=255)
                                                     gTTCGCCTAACGTAATGGTGTTGACAAAACCGGTGAGGTGGTCGCGCAAATCACGCCACAGCTCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGgcg                                                                                <  2:781292/143‑1 (MQ=255)
                                                                  aaTGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTCATTACTTCGc                                                                   >  2:403685/1‑143 (MQ=255)
                                                                  aaTGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTACTTACTTCGc                                                                   >  1:707807/1‑143 (MQ=255)
                                                                         ttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCGCCGGc                                                            >  1:91521/1‑143 (MQ=255)
                                                                           gagaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGTCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTCATTACTTcgccgacgccga                                                          >  1:491820/1‑143 (MQ=255)
                                                                            ggtatgccGGTGGGACGGTCGCTCAAAGCACGCGTGGGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGTTTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAt                                                         <  2:392753/137‑1 (MQ=255)
                                                                            agagagccGGTGTGTCGGTCGCTCCAATCACCCCACAGCGGGTGGGTCATGCATTTATCGTCGCCCTGTCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACATATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAt                                                         <  2:228540/137‑1 (MQ=255)
                                                                                aaCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtgctg                                                     >  1:503889/1‑143 (MQ=255)
                                                                                 agcGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTTCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtgctgc                                                    <  1:44255/141‑1 (MQ=255)
                                                                                    ggTGCGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTgg                                                 <  1:655062/143‑1 (MQ=255)
                                                                                      tGAGACGGTCGCCTCATTCGCGCCCCAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCa                                               <  2:653414/143‑1 (MQ=255)
                                                                                      ggggccGGTGGCTCACAGCTCGCCGCAGCGCGGGGGTCATGCATTTCTCGTCGCCCTGGCAGCCGCCTCTTCGCTGACAACGGGTGGCATCGACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCa                                               <  2:614591/138‑1 (MQ=255)
                                                                                       tcaccGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGG‑ATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATc                                             >  1:472500/5‑143 (MQ=255)
                                                                                             gTCGCTCAAATCACGCCACAGCGCGTGGGTCCGGCATTTATCGTCGCCCTGGCAGCCGCCTTTCCCCTGACAACGGGTGGCATCTACAGATTCGTCCACGGCGCTAATTACTTCGCCAACGGCCAGGCTGCTGGCAtgtgcgc                                        >  2:477156/1‑137 (MQ=255)
                                                                                             gTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTGCTTCGCCAACGGCCATGCTGCTGGCCTCGTTGc                                        >  1:132903/1‑143 (MQ=255)
                                                                                                  tCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAAc                                   >  1:433676/1‑143 (MQ=255)
                                                                                                  tCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAAc                                   >  2:603722/1‑143 (MQ=255)
                                                                                                              acaGCGCGTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGAGGCATCTACAGCTTCGTCAACGGCGCTAATTCCATCGCCAACGGCGATGCTGACGACCTACTTGCCTAACAGATAACCACCg                       >  2:318868/1‑143 (MQ=255)
                                                                                                                ggcgcgcGGGTCAGGCAATGAATGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGcc                     <  1:211498/142‑1 (MQ=255)
                                                                                                                     gTGGGTCAGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACACTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGATGCTGGCATGTTTGCCTAACAGACAACCACCGCCTGGTc                >  2:461939/1‑143 (MQ=255)
                                                                                                                            aGGCATTTATCGTCGCCCTGGCAGCCGCCTTTACAATGACCGCCGGTGGCATCTACAGATTCGTCAACGGCCCTCATTACGTCGCCAACGGCGAGGCGGCGGGCATCTTTGCCTAACAGGTAGCGACGGCCGGGTCCAGGTag         >  2:640261/1‑142 (MQ=255)
                                                                                                                                 tttATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTgg    >  1:581357/1‑143 (MQ=255)
                                                                                                                                   tATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAATGTGTGGCATCTACTGATTCGTTAACTGCTCTATTTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCTCCGCCTGGTCCACGTACGCTGGaa  <  2:381389/143‑1 (MQ=255)
                                                                                                                                   tATCGTCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGaa  <  1:768440/143‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
GTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAA  >  NC_000913/2661694‑2661967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑