Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,232	C→T	T370T (ACC→ACT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,232	0	C	T	94.1%	42.5 / ‑6.4	17	T370T (ACC→ACT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (7/9);  total (7/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTT  >  NC_000913/1196099‑1196300                                                                                                                                      |                                                                     cAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCt                                                             >  1:520469/1‑143 (MQ=255)    aCATCGGTGACGAATGCGCCCTGTTTGAAGCCAACAACGGTACTGCGACGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCAGAGATGATGCTGCACCATATAGGTAAGACTGAAACGGcatac                                                          >  2:485090/1‑139 (MQ=255)                aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                              <  2:199612/143‑1 (MQ=38)                aaTGCGCCCGGGTTGAGGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                              <  2:502805/143‑1 (MQ=37)                 aggcgcCCTGTTTGAAGCCCCCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                             <  1:78400/141‑1 (MQ=37)                 aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                             <  1:734219/143‑1 (MQ=38)                       ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGACACGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg                                       >  1:359947/1‑143 (MQ=37)                        cTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGGAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAGTGTTAAAGGTATGGa                                      >  1:403150/1‑143 (MQ=37)                           tttGAAGCAACCCTCGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg                                   <  2:667088/143‑1 (MQ=25)                            ttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgc                                  <  1:501489/143‑1 (MQ=255)                              caaGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAAGCCTGTCTGTATTATTCTCTGCGCTGTGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcgc                                <  2:251370/142‑1 (MQ=21)                                aGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGCTAAAGGAATGGGAGTCGCaa                              >  2:301670/1‑143 (MQ=37)                                     cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                         <  1:575113/143‑2 (MQ=25)                                      ccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCAATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACATGATTGTTAAAGGTATGGAAAGCGCAAACaatg                        >  2:245217/1‑141 (MQ=25)                                          ggTACTGCTCCGAGAGACGCCCGTCAGGACAAAGTAAATCCTGGCTCTATTATGCTCTCCGCTGAGATGCTGCTGCGCCATATGGGTTGGACCGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa                    <  2:541264/143‑1 (MQ=18)                                                            gCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt  <  2:357367/143‑1 (MQ=17)                                                            gCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCCGCTGACCTGATTGTCAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGAcct  >  1:605992/1‑141 (MQ=17)                                                                                                                                      |                                                                     CAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTT  >  NC_000913/1196099‑1196300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑