| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,351,007 | Δ1 bp | 35.3% | coding (2019/2337 nt) | arcB ← | sensory histidine kinase ArcB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,351,007 | 0 | A | . | 35.3% | 22.9 / 17.3 | 17 | coding (2019/2337 nt) | arcB | sensory histidine kinase ArcB |
| Reads supporting (aligned to +/- strand): ref base A (8/3); new base . (3/3); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01 | |||||||||||
ATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCG‑CTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTATCCCAGAATTTCTTGATCATCGCGGTTAGCGCCGGAACAGAAAGCGGCTTACTC > NC_000913/3350870‑3351148 | aTTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCG‑CTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATAcgg > 1:229940/1‑141 (MQ=255) ttttATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCG‑CTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGt > 1:11841/1‑143 (MQ=255) tccttcctCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCG‑CTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCa > 2:128861/1‑143 (MQ=255) ttttATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCCTCATCTTCTCAAACACAGAATATGCCGT‑GGTGATCTGCTCCGGTCCTACACGTTCGGGATCCTGTTCCAGCATGGGCATCTCGAGCACTGCt > 2:140647/1‑143 (MQ=255) ttttTCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCTGCATCATCTTCTCAAACACCG‑CTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCtt < 2:118191/143‑1 (MQ=255) ccAGCCCGCCGACACGGCCCGGCAGCATCTTCTCAAGCACCG‑CTGACCCGTCGGCGAGCAGCTTCGGTACTACAAGTTCGAG‑TACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCa < 2:192245/143‑1 (MQ=255) gccACCCTGACATAGCCCTCCACCATCTTCTCGACCACAT‑GGACCTCTTCGGTGATCAGCTTCGGTCCTACAAGTTCGAG‑TACTGTTCCAGCATGGGAATATCGAGCACTGCTTCTGATTTACTGTTCTCTTCTGTCGTCAcc < 2:174416/141‑1 (MQ=255) gcgtgCTGTCATAGCCCGGCTGCTTCTGCTCAACCCCCG‑CTAGCCCGTCGGTCGTCAGCTTCGGTCCTAGAAGTGCGTGATACTGTTCCAGCATTGGAATATCGAGCAATGCTTCTGTTTTACTGTTCTCTTCTGTCGTCAcc < 2:381458/139‑1 (MQ=255) gCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCG‑CTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAG‑TACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTCCTCTTCGGGCGTCACCg > 1:53021/1‑143 (MQ=255) cACGCTGACATAGCCCGGCATCATCTTCTCAAACACCG‑CTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCg > 1:4914/1‑143 (MQ=255) atcatcTTCTCAAACACCG‑CTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAG‑TACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTgg < 1:61764/143‑1 (MQ=255) atcTTCTCAAACACCA‑CTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAG‑TACTGTTCCAGCGTGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGGAATCTCCTCATCATCCTGGGTa > 1:397972/1‑143 (MQ=255) tcttctCAAACACCG‑CTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTa > 1:170664/1‑143 (MQ=255) aaaCACCG‑CTAACCCGTCGGTGATCAGCTGCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTATCCCAGa < 1:128861/143‑1 (MQ=255) gCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTATCCCAGGATTTCTTGATCATCGCGGCTCGCGcc > 2:42593/1‑143 (MQ=255) cTACAAGTTCGAG‑TACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTATCCCAGAATTTCTTGATCATCGCGGTTAGCGCCGGAACAGaaa > 1:234914/1‑143 (MQ=255) cAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTATCCCAGAATTTCTTGATCATCGCGGTTAGCGCCGGAACAGAAAGc > 2:160929/1‑143 (MQ=255) acttctggaTACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGGTTTACTGTCCTCTTCTGTCGTCACCGTACTCTCCTCACCATCCTGGGTATCCCAGAATTTCTTGATCATCGCGGTTAGCGCCGGAACAGAAAGCgg < 2:25050/136‑1 (MQ=255) gaTACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCCCCGTACTCTCCTCATCATCCTGGGTATCCCAGAATTTCTTGATCATCGCGGTTAGCGCCGGAACAGAAAGCGGCTTACTc > 1:257931/1‑143 (MQ=255) | ATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCG‑CTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTATCCCAGAATTTCTTGATCATCGCGGTTAGCGCCGGAACAGAAAGCGGCTTACTC > NC_000913/3350870‑3351148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |