| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 272,330:1 | +G | 15.4% | intergenic (+140/‑250) | insN → / → eyeA | CP4‑6 prophage; IS911A regulator fragment/CP4‑6 prophage; small RNA EyeA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 272,330 | 1 | . | G | 15.4% | 70.8 / 13.1 | 26 | intergenic (+140/‑250) | insN/eyeA | CP4‑6 prophage; IS911A regulator fragment/CP4‑6 prophage; small RNA EyeA |
| Reads supporting (aligned to +/- strand): ref base . (9/13); new base G (2/2); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-02 | |||||||||||
TGCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAAC > NC_000913/272194‑272466 | tgCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATg < 1:250515/143‑1 (MQ=255) cccGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGGCATGc > 1:195680/1‑143 (MQ=255) ccGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATAACACCGGGTGTGACGGTGGAGCACCTGAGCGGCAGTGACCTGC‑GGCATGccc > 1:170808/1‑143 (MQ=255) aGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGGCATGccgcag < 2:195680/143‑5 (MQ=255) taaGGGGGGGGAGGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGGCATG‑CCGCAggtc < 1:215578/141‑3 (MQ=255) tCAGGGCGGGCATGGGTGCTTAAATGAACCGCGAGTCTGGCTGGAATATTGAACCGGTATCTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGt < 2:12982/143‑1 (MQ=255) gcATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGACAGCGAACTGC‑GGCATGCCAGCAGGGTGATGTAccc > 2:287636/1‑143 (MQ=255) ggATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAAGCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCt < 1:6179/143‑1 (MQ=255) ttAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACCAc > 2:42274/1‑143 (MQ=255) tGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGCGATGTCACCCGCGGACAACggggg > 2:303638/1‑142 (MQ=255) tGAACCGGTAACGCACGAGGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCgaga < 1:150823/143‑1 (MQ=255) gTAACGCACGATGTGAAGCCCAACCGTCCCACCGGGCGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCGTGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCt < 2:25016/143‑1 (MQ=255) tCACGATGAGAAACCCAACAATCCCACCGGGTATGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGGGTTGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCTAGATGGTCCGCAggg > 1:376118/1‑143 (MQ=255) aTCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTgaa < 2:363354/143‑1 (MQ=255) cACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAAc < 1:382504/143‑1 (MQ=255) cACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAAc > 1:369604/1‑143 (MQ=255) tgACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGACAGGTTGAAGAACATGAACCCg > 2:319187/1‑143 (MQ=255) aCGGTGGAGAACCTGACCGGCAGTGACCTGC‑GGCATGCCCGCACGGTGATGTAACCCGCTGACAACGGGGATTGAGCCGCGATCACTAATCCGAGCTGATCCTCCACGTTAAGTACTGACTGGTTGCAGAACATGAACCCGtt > 1:388687/1‑143 (MQ=255) aGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTGTGTgg < 2:330710/143‑1 (MQ=255) cGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGt < 1:60431/143‑1 (MQ=255) ggCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGtt < 1:369980/143‑1 (MQ=255) ggCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTGACTCCGCCCCTGCgggac > 2:216601/1‑141 (MQ=255) gACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAAc < 2:211475/143‑1 (MQ=255) gACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAAc < 1:271675/143‑1 (MQ=255) gACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAAc < 1:42274/143‑1 (MQ=255) acccTGCGGGCATG‑CCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAAc > 2:288249/3‑143 (MQ=255) | TGCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATATTGAACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGC‑GGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAAC > NC_000913/272194‑272466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |