| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,085 | T→A | 100% | I690N (ATC→AAC) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | A | 100.0% | 41.4 / ‑6.7 | 17 | I690N (ATC→AAC) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (9/7); minor base C (1/0); total (10/7) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGG > NC_000913/124945‑125190 | ttgTCACAGCCACATTCAGTCGCTGACGATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTacaaca < 2:166594/141‑1 (MQ=255) tCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTGCGGTGAAAAACAAGAGAACGTTTACTACTACAACACTa > 1:246809/1‑143 (MQ=255) cggTCGCTGACTATCCCGGACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCAGGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACGCTACGCTGTACGaaaa < 2:370440/141‑1 (MQ=255) tCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGATTACTACTACAACACTACGCTGAACGacacctt > 1:350359/1‑137 (MQ=255) gACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTCCACCACTACGCTAAACGAAAACTACcaag > 2:90673/1‑141 (MQ=255) tATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCGTCATGCATGACGGTCTGGAGCGTATGTACGGTGAAACACAAGAGAACGTTTACTACTACAACCCTACGATCGAAGAACACATCCACATGc > 2:319200/1‑143 (MQ=255) tCCCGAACTGTATCTCTTACGCCCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCg < 2:253075/143‑1 (MQ=255) cccGAACTGTATCGCTTACGACGCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCgg < 1:263186/143‑1 (MQ=255) cTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACCACACTACGCTGAACGAAAACTACCACATGCCGCCAATGc > 2:307361/1‑143 (MQ=255) tGTACCTCTTACGTCCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGcc < 2:343065/143‑1 (MQ=255) gTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCg > 2:359215/1‑143 (MQ=255) tctcTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGACGGaa > 2:109463/1‑143 (MQ=255) ggcgcacgctaacgAAGGTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGCTTACTACTACAACACGACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAg < 2:293904/138‑1 (MQ=255) agggaGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTaaa < 1:233107/139‑1 (MQ=255) tgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACAACATGCTTGTAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATATACAAACTCGAAACTa > 2:238145/1‑143 (MQ=255) ggTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGa < 1:245798/143‑1 (MQ=255) gAGCATATGTACGGTGAAAAACAATAGAACGTTTACTACTACAACACTACGCTGAACGAAGACTACCACATTCCGGCAATGCCGGAAGGTGCTGAGAAATCTATCCGTAACGAGATCCACAAAATCTCAACTATCGAAgccca > 2:286954/1‑139 (MQ=255) aTGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCCGCAATGCCGGACGGTGCTGAGGAAGGTATCCGTAAAGGCATCGACAAACTCGAAACTCTTGCAGGCAGCAAAgg > 2:280557/1‑143 (MQ=255) | TGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGG > NC_000913/124945‑125190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |