| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,570,512 | C→T | 100% | intergenic (‑23/+133) | gadC ← / ← gadB | L‑glutamate:4‑aminobutyrate antiporter/glutamate decarboxylase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,570,512 | 0 | C | T | 80.2% | 38.3 / 1.9 | 20 | intergenic (‑23/+133) | gadC/gadB | L‑glutamate:4‑aminobutyrate antiporter/glutamate decarboxylase B |
| Reads supporting (aligned to +/- strand): ref base C (2/2); new base T (13/3); total (15/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
TTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTA > NC_000913/1570381‑1570644 | ttGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTTCTGTCGGAAcc > 2:289476/1‑143 (MQ=255) tAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAg > 1:369154/1‑143 (MQ=255) aaaCAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTTCTGTCGGAACCGCACCTGTTTTGTTCTGAAGc > 1:276565/1‑143 (MQ=255) aaCAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTTCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCg > 2:158955/1‑143 (MQ=255) aCAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTTCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGt > 1:251752/1‑143 (MQ=255) aCAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGGATTTCTGTCGGAACCGCACCTGTTTTGTTCTGGAGCGt > 1:163841/1‑143 (MQ=255) tACCCTCGATGCTGTTATTGCAAAGTATCCACGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTTCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGaa < 2:154712/143‑1 (MQ=255) ctTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTTCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGACATTGAACAGACATTGGAAATTg > 1:286358/1‑143 (MQ=255) tttACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTTCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCAGa > 1:391774/1‑143 (MQ=255) tttACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTTGAAATTGTCCCGa > 2:244124/1‑143 (MQ=255) aCTGATGTAGCCATATTATCCCCCTAAAACGGTATTTCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGttt > 1:319584/1‑143 (MQ=255) tggtgcaGCCATATTATCCCCCTACCACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATGCAGAGCAATATTGTCCGTTGCTAATGCCAGTGAACGGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCg < 1:144261/137‑1 (MQ=255) tGATGTAGCCATATTATCCCCCTAAAACGGTATTTCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGTAAATTGTCCCGCAACGGGTCCGTTTCg > 1:178553/1‑143 (MQ=255) tGATGTAGCCATATTATCCCCCTAAAACGGTATTTCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCg < 1:158955/143‑1 (MQ=255) aTGTAGCCATATTATCCCCCTAAAACGGTATTTCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCGGAAACGGGTTCGGTTCggg > 2:28860/1‑143 (MQ=255) ccATATTATCCCCCTAAAACGGTATTTCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGGACAGACTTTCGAAATTGTCCCGAAACGGGTTCGTTTCGGGACaccg > 1:313712/1‑143 (MQ=255) tCCACCTAAAACGGTCTTTCTGTCGGAACCGCACCTGTTTTGTTCCGAAGCGTATGCAGAACAATCTTTTCCGTTGCTAATGCCAGTCAACAGACTTTGGAAATTGTCCCGACACGGGTTCGTTTAGGGACaccgttaccgct > 2:285737/1‑141 (MQ=255) cccTCAGACGGTATTTCTGGCGGAACCGCACCTGTTGTGTTCTGAAGCGTATTCAGAACAATAGTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTaaa < 1:348141/143‑1 (MQ=255) cccTAAAACGGTATTTCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTaaa > 2:163691/1‑143 (MQ=255) agtcGGGATTCCTGTCGGGACCGCAACTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTa < 1:120412/140‑1 (MQ=255) | TTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAACGGGTTCGTTTCGGGACACCGTTACCGTTAAACGTTA > NC_000913/1570381‑1570644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |