| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,661,816 | T→G | 71.0% | T106P (ACC→CCC) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,816 | 0 | T | G | 71.0% | 9.2 / 6.2 | 14 | T106P (ACC→CCC) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base G (4/6); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01 | |||||||||||
GGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAT > NC_000913/2661681‑2661912 | gggTGCGTGGCTCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTCACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCAt < 2:234289/143‑1 (MQ=255) gCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACAGCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTAt < 2:58349/143‑1 (MQ=255) gcgcGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATcgccg > 2:259917/1‑143 (MQ=255) cgcgTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATcgccgc < 1:142966/143‑1 (MQ=255) cgtGAGTAGGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTgg < 1:56892/143‑1 (MQ=255) aTGCTGACGACCAGACTCATCCAACACTTCCTCTTTATTAACCAGTTCGCCTAAAGTAATTTTGTTGAGAAAACCGGTGGGACGGTCGCTCAAAGCACGCCCCAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAgccgc < 2:77232/143‑1 (MQ=255) gCTGACGACCAGACACATCCAGCACTTCCTGGTTAGTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCt < 2:270879/143‑1 (MQ=255) cTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGCAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCCCGCAgccgccct > 2:258201/1‑141 (MQ=255) tctgttCCTAGTTGTTACCGAGTTCGCCTCAGGTATTGGTGTTGAGAAAGCCGGTGAGACGGTCGCTCAGCTCCCGCCACGGCTCGGGTGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCa < 2:367484/139‑1 (MQ=255) cTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATGTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAg > 1:102889/1‑143 (MQ=255) tGGTTATTGACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGTCTTTACCCTGACAACGGGTGGCATCTACAGa < 1:205346/143‑1 (MQ=255) tGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGa > 1:177267/1‑143 (MQ=255) taACCAGTTCGCCTAAAGTAATGATGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGCGGCATCGACAGATTCGCCa > 2:241315/1‑143 (MQ=255) agaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTCATTACTTCGCCAACGGCgct > 1:324498/1‑141 (MQ=255) | GGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAT > NC_000913/2661681‑2661912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |