| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,398 | T→G | 100% | L29R (CTT→CGT) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,398 | 0 | T | G | 100.0% | 59.5 / ‑6.7 | 24 | L29R (CTT→CGT) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (14/8); total (15/9) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑TTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCT > NC_000913/3041274‑3041536 | tAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGgcgc < 2:181545/143‑1 (MQ=255)tAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATCGCTTTTACACCTTTTCCTCCCCTTCATCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGTGCGC‑GTGCCACCATTACTGgcgc < 2:130568/143‑1 (MQ=255) tACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGCTGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGaa > 2:162584/1‑143 (MQ=255) tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGACAAATATATGCAGGGTCAGGTGAcag > 2:291176/1‑143 (MQ=255) tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcag > 1:253910/1‑143 (MQ=255) tatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAAGATATGCAGGGGCGGGGGAcagcag > 1:363081/1‑143 (MQ=255) cTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACAGTCACCGCTGCTGACGCTTGATGACTGGGCGC‑GGGCCACTCTTAGTGGAGCCGACAGCGACAAACGTCTGCAGGGTGCGGTGAccgcagcggcc > 2:157197/1‑138 (MQ=255) cacCTTTTCCTCCCCGTCAGCCTACGGATTCTGCCCGTTTATCGCTGACGCTGATGACGCTGGATGACTGGGCCCGCTGCGGCC‑GTACTGGCCCGGACCGCGAAGAAGATCTGCCGGGTCAGGGCACAGGAGAGCTGAGCCAg > 2:119551/1‑143 (MQ=255) cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGCTGTGAGCCAGATg > 1:333332/1‑143 (MQ=255) cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATg > 1:113762/1‑143 (MQ=255) tttcCCTCCCCGTCAGCCTACGGCGTCTGCCCGGTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATgg < 2:118049/143‑1 (MQ=255) cctcgccccGTCAGGCGACGGCTTCTGCGCGTTGAGCGCTGACGGTGATGCCGCTTGATGACTGGGCGC‑GTGCCGCCATTACGGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCGAGATGGCa < 2:123467/138‑1 (MQ=255) tcctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCag > 1:339906/1‑143 (MQ=255) ctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGACGTCAGCCCGATGGCagaa > 2:218113/1‑143 (MQ=255) ctccCCGTCAGCCCACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaa < 1:122258/143‑1 (MQ=255) tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag > 1:254522/1‑143 (MQ=255) ccGTCAGCCTCCGGCTTCTGCCCTTTTACCGCTGACGCTTATTACTCTTTATGACTGGGCGC‑TTGCCACCATTTCTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc < 1:125662/143‑1 (MQ=255) tCAGCCTACGGCTTCTGCCCGTTCACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGc < 1:99498/143‑1 (MQ=255) gCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgc > 1:211517/1‑143 (MQ=255) ttCTGCCCGGTTACCGCTGACTCTGATGAAGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgccg < 2:12894/143‑1 (MQ=255) ttACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGa > 2:140147/1‑143 (MQ=255) cGCTTGATGACGGGGCGC‑GTGCCACCATTACTGGCGCGGCCAGCGAAAAATATATGCAGGGTGCGGTGGCGGCACAGCCCAGACAATAATTTTAATAGTTGGAGCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTgg < 1:291176/143‑1 (MQ=255) ttGATGACTGGGCGC‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAACTGTGGAGc > 2:313390/1‑143 (MQ=255) gcgc‑GTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGCTGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCt > 1:388323/1‑143 (MQ=255) | TAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGC‑TTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCT > NC_000913/3041274‑3041536 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |