| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,137,914 | C→T | 8.3% | intergenic (‑257/+18) | yijF ← / ← gldA | conserved protein YijF/L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,137,914 | 0 | C | T | 8.3% | 129.5 / 2.8 | 51 | intergenic (‑257/+18) | yijF/gldA | conserved protein YijF/L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (22/24); new base T (2/2); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
ATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTT > NC_000913/4137773‑4138056 | atcaCTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGcc < 1:214429/140‑1 (MQ=255)aTTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGcc > 2:261658/1‑143 (MQ=255)aTTACTGGCACAGAGTGCCGGATGCGCAGTGAACGCCTTAACCGTCCTACAAAAGCACGCAAATTCAATACATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGCCAAGcc > 2:262624/1‑143 (MQ=21) ccgaagTGCGGGTTGCGTCGGGAGCGCCTTATCCGGCGTACAAAAGCACGCAAATTTAATATATAGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGTGAGTTTg < 2:330762/139‑1 (MQ=18) gTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTa < 1:24795/143‑1 (MQ=255) gTGCCGGATGCGGAGTGAACGCCATATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTa > 1:382025/1‑143 (MQ=255) ggaggtaaCGCCTGATCCGGCCTACATAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGGGTTTGGAGTAGGTTATTCCCa < 2:189858/137‑1 (MQ=21) ggAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGACAATTTTGCGTTAAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCa > 2:305346/1‑143 (MQ=255) gTGAACGCCTTATCCGGCCCACAAAAGCAGGCAAATTCAATATACCGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCActc < 1:361026/143‑1 (MQ=255) cGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCa < 1:373741/143‑1 (MQ=255) ctgaTCCGGCCTACAAAAGCACGCAAATTCAATATATGGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGa < 1:84829/140‑1 (MQ=255) ccGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGCAGGTTATTCCCACTCTTGCAGGAAACGc > 2:64356/1‑143 (MQ=255) cGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGTCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCt > 2:157454/1‑143 (MQ=255) cGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGTCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCt > 2:156273/1‑143 (MQ=255) cGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCt > 1:97043/1‑143 (MQ=255) ccTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGAc < 1:100132/143‑1 (MQ=255) tACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCACACTCTTACAAGAAACGTTGACCg > 1:180392/1‑143 (MQ=255) acgCACGCAAATTCAATATATGGCAGGGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTg < 2:4604/141‑1 (MQ=255) aaGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTg < 1:269638/143‑1 (MQ=255) aaGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTGATTCCCACTCTTGCAGGAAACGCTGACCgcatcg > 2:134924/1‑138 (MQ=255) aaGCACGCAAATTCAAGATAGTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGCTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTg < 1:257133/143‑1 (MQ=255) aCGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCg < 2:382025/143‑1 (MQ=255) cGCAAATCCAATATTTTGCAGAGATTGCGGAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCATGAAACGCTGACCGTACTGGTCgg < 1:168723/143‑1 (MQ=255) gCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGc < 1:320454/143‑1 (MQ=255) aaaTTCAATATATTGCAGAGATTACGTAGGCGTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTa > 1:251009/1‑143 (MQ=255) atgttGCAGAGGTTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCagag < 1:380707/140‑1 (MQ=255) atatTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCagag > 1:121138/1‑143 (MQ=255) atTGCTGTGATTGCGTAAGCCAGATACGCGTAGCCCACCAGGCAATTTCGCGTTCGTACTCCCGGACAAGCCGGGAGTTTGGAGTAGCTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAgcg < 1:104974/143‑1 (MQ=255) cAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGt > 1:304932/1‑143 (MQ=255) agagATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTa > 2:77907/1‑143 (MQ=255) agaTTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTaaa > 1:257034/1‑143 (MQ=255) agaTTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGAAGGAAACGCTGACAGACCTGCACGGCTACCAGCGGCGCGACGTaaa > 2:257440/1‑143 (MQ=37) aTTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCCGAAAc‑c > 1:38670/1‑143 (MQ=255) cGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACACGCCGGGAGCTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGGCCGTGCTGGTCGGCTACCATCAGAGCAGCGGAAAC‑CCGAt > 2:274987/1‑143 (MQ=38) gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATc > 1:349708/1‑143 (MQ=255) aGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTg < 1:174715/143‑1 (MQ=255) ggCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTgg > 2:230675/1‑143 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGTCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGc < 1:156273/143‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGTCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGc < 1:157454/143‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTcgc < 1:174818/143‑1 (MQ=255) tAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGCGTTTGGAGTAGGTTATTTCTACGCTTGCAGGAAACGCTCACCGTACTCGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTcgcg > 2:387411/1‑143 (MQ=255) cATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAGAC‑CTGACCTGGCGTCGCGCCGCCAGGCAt > 2:50384/1‑143 (MQ=255) gCAATTTCGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTtgtg > 1:361906/1‑143 (MQ=255) cAATTTTGCGTTCCAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGa < 1:261658/143‑1 (MQ=255) cAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGa < 1:220256/143‑1 (MQ=255) cAATCTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGa < 1:262624/143‑1 (MQ=255) tGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGtt > 1:241841/1‑143 (MQ=255) ttCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCCGAGCAGCGCACAC‑CGGATCTGCCGTCGCGCCGCCACGCCGCTTGTGAACGGTCTCAc > 2:159889/1‑143 (MQ=255) aaaCTCCCGGACAAGGCGGGAGTTTGGAGTAGGTTATTCCCGCTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCtt < 1:209906/143‑1 (MQ=255) aaaCTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCtt < 1:314646/143‑1 (MQ=255) tCCCGGACAAGCCGGGAGTTTGGAGTAGGTTGTTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGc < 1:161092/143‑1 (MQ=255) cGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCCGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCCGcaca > 2:261740/1‑143 (MQ=255) ctaaGCCGGGAGGTTGTAGTAGGTTATTCCCACTCTTGCAGGAAACGCCGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGc < 1:252672/141‑1 (MQ=255) aaGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCg < 1:230675/143‑1 (MQ=255) ccGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTCGTCGGCTCCCAGCTGCGCGGCCTACTCTCCGTACT‑CCGACACGGCGCCATGCATGTTGTGAATGGTTTAACATTCTGCACATGCCGAtt > 1:90655/1‑143 (MQ=255) | ATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAC‑CTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTT > NC_000913/4137773‑4138056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |