Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	699,559	A→G	26.1%	intergenic (‑382/+15)	asnB ← / ← umpH	asparagine synthetase B/UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	699,559	0	A	G	26.1%	18.5 / 3.1	15	intergenic (‑382/+15)	asnB/umpH	asparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base A (6/5);  new base G (2/2);  total (8/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

CCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTG  >  NC_000913/699426‑699689                                                                                                                                      |                                                                                                                                   ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCTGCGGACGTGGtt                                                                                                                           <  1:327092/143‑1 (MQ=255)        cgccgTGAACGCCTCATCCCGCCTACAAAAGCAGGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACTACGTCCGCAGACGTGGTTGCTATTc                                                                                                                    <  2:143146/140‑1 (MQ=25)                  gCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATCACGTc                                                                                                          >  2:65122/1‑143 (MQ=255)                       aTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGAttt                                                                                                     <  1:281390/143‑1 (MQ=255)                           ggCCTACATAGTCATGAAAATTCAATATATTGCAGTAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGc                                                                                                 <  2:131609/143‑1 (MQ=37)                           ggCCTACAAAAGCATGAAAATGCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGc                                                                                                 <  2:10611/143‑1 (MQ=255)                                                    tatatTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTgggg                                                                        <  1:175220/143‑1 (MQ=255)                                                                  tGCGTAGGCCTGATAAGCGTAGCGCCTCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACt                                                          >  2:390677/1‑143 (MQ=255)                                                                       aGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGa                                                     >  2:328380/1‑143 (MQ=255)                                                                                   cGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGcg                                         >  1:177484/1‑143 (MQ=255)                                                                                                            ttGTCGTCGGAGCCAACCCTGTGCGGAGACTTGGTTGCTATGCAGTTAACGTCGATTTCAGCGACTGACGAGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAAt                <  2:349243/143‑1 (MQ=255)                                                                                                                  tcaGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGCAAATCCAGCTGGGGCGGAAAGGCATACTGCCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGtctc          >  2:44750/1‑143 (MQ=255)                                                                                                                          aaCCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGAcctg  >  1:148972/1‑143 (MQ=255)                                                                                                                          aaCCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTAGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGGCGAACCACCAGAAAGCGCCAGCATCGTCTCCAGGcctg  >  1:34829/1‑143 (MQ=255)                                                                                                                          aaCCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGACGATATCGACGAACGACGAAACACCCGAAAGCACCAGAATCGTCCCCCGAcctg  >  1:49621/1‑143 (MQ=255)                                                                                                                                      |                                                                                                                                   CCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTG  >  NC_000913/699426‑699689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑