| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,661,816 | T→G | T106P (ACC→CCC) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,816 | 0 | T | G | 97.8% | 121.2 / NA | 46 | T106P (ACC→CCC) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (25/20); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTC > NC_000913/2661685‑2661953 | aagTGGAGCGCCGGGAGGACGCAGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCGAAAGTAAGGTTGTGGAGAAAACCGGTGAGACGGGCGCACAAATCACACCACAGCGCGTGGGGCAGGCATTTAt < 1:246857/141‑1 (MQ=255) ggTGGCGAGTCGTGAGTATGCTCACGACCAGACACATCGTGCACTTCCTGGTTATTAACCAGTTAGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATc < 1:992677/142‑1 (MQ=255) gcgcGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCCCAAATCACGCCACAGCGCGCGCGGCAGGCATTTATcgccg > 2:1262226/1‑143 (MQ=255) gcgTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGACTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCgccgcc > 1:659330/1‑143 (MQ=255) gCTGACGACTATACACATCCAGTACTTCCTTGTTATTACCCAGTTCGCCTAAAGGAAGGTCGTGGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCt < 1:1157602/143‑1 (MQ=255) acgacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGATAGCTCAAATAACGCCACAGCGCGTGGGGCAGGCATTGATCGCAGCCCTGGCAGCCGCCTTTAc > 1:37635/1‑143 (MQ=255) cgcgcAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTAcc < 2:683808/139‑1 (MQ=255) ccacCAGACACATCCATCTCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTAcc < 2:461570/141‑1 (MQ=255) gACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCAAAGAGCGTGGAGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACa > 1:997246/1‑143 (MQ=255) caTCCAGCACTTCCTGGTTATTACCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACgg < 2:225507/143‑1 (MQ=255) aTCCAGCACTTTCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGTGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGGGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACggg < 1:716557/143‑1 (MQ=255) aTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATTTCGGAACAACGCGTGGGGCAGGCATACATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACggg > 1:446479/1‑143 (MQ=255) tCCAGCTCTGTCGGGTTCTTAGCTAGTTCGGCTCAAGTAATGTTGTTGAGTATACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGt < 2:959295/143‑1 (MQ=255) ccAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTg > 2:1104734/1‑143 (MQ=255) taGCACTTTCTGGTTATTAACCTTTTCGCCTAAAGTAATGTTGTTTTGAAATCCGGTTAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGAATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTgg < 1:631615/142‑1 (MQ=255) aGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAATCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCACTGGAAGACGCCTTTACCCTGACAACGGGTGGc > 1:686584/1‑143 (MQ=255) tGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGa > 1:754442/1‑143 (MQ=255) ttattaTCCAGTTCTCTTAAGGTAATGTTGTTGTGAAATCCGGTGAGACGGTCGCTCAAATTACGCCACAGCGCGTGGGGCAGGCATGTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACATCGGGTGGCATCTACAGATTc < 2:584648/143‑1 (MQ=255) attaattaGTTCGCTTAAATTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCGAGAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGt < 1:1202916/143‑1 (MQ=255) ttaACCATTTCGCCTTATGTATTTTTTTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTc < 1:1258999/143‑1 (MQ=255) ttaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTc < 2:754442/143‑1 (MQ=255) aaCCAGTTCGCCTAAATTAATGTTGTTGAGCAATTCGTTTAGATGGTTGCTCAAATCACGACACAGCGCGTGGGGCAGGCATTGATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCaa < 1:52740/143‑1 (MQ=255) aaCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTAAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTCGCAGCCGCCGTTACCCTGACAACAGGTGGCACCTAAAGATTCGTCaa > 2:479754/1‑143 (MQ=255) ccAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCACACTGGCAGCCGCCTTTACACTGACAACGGGAGGCATATAAAGATTCGTCAACg > 1:1098675/1‑143 (MQ=255) gTTCGCCTAAAGTACTGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCCTTTATCGCCGCCCAGGCAGCCGACTTTACCCTGACAACGGGTGGCATCCACAAATTCGTCAACGgcg > 2:146748/1‑143 (MQ=255) atAAACTAATGTTGTTGAGAAAACCGGTTATTCGGTCGCTCTAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAAtt < 1:721766/142‑1 (MQ=255) tAAAGTAATCATGTTGAGAAAACAAGAGAGACGGTCGCTCAAATCACGCCACAGCGCCTGAGGCAGGCATCTATCGCCGCCCTGGCAACCGCCTCTACCCTGACAACGCGTGGCATCTACAGCTTCGTCAACAACGCTaacga > 2:760722/1‑140 (MQ=255) aaaGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTAc > 2:775125/1‑143 (MQ=255) aGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACGCTCACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACtt > 2:414437/1‑143 (MQ=255) gTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTAACACTAACAACGAGTGGCATCTAAAAATTCATCAACAGCGCTAATTACTTc > 1:539544/1‑143 (MQ=255) ttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGAATCGTCAACGGCGCTAATTACTTCGCCAACGGc > 1:1223593/1‑143 (MQ=255) agaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTGCGCCAACGGCGAt > 2:1037216/1‑143 (MQ=255) agaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATGACTCCGCCAAGGGCGAt > 2:347317/1‑143 (MQ=255) aaCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCTTTCCCCCCGCCCCCCGCCCCCATCTACGGATCCGTCAACTGCACTAATTACTTCGAAAAAGGCGAtgata > 1:908929/1‑140 (MQ=255) ggTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTgg > 1:812564/1‑143 (MQ=255) gaCGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAAAGAGAGGCAACAACAGATTCGTCAAACGCGCTAGGAACTTCGCCAACGGCGATGCTGCTGGCATCt > 1:970151/1‑143 (MQ=255) aCGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGTATTTATCGCCCTTCTATCATCCACCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCtt < 1:270079/143‑1 (MQ=255) gggTCGCTCAAATCACGCCACAGCGCGTGGGGCGGGAACATACCTCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCttt < 2:1223593/142‑1 (MQ=255) cGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTTCCTTGATAACGTGTGGCATTTACAGATTCGTCAACGGCGCGAAGTACTGCGCCAACGGCGATGCTGCTGGCATCttt < 1:572202/143‑1 (MQ=255) tCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGcc > 2:1236267/1‑143 (MQ=255) gCTCAAATCACGCGACAGCGCGAGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCACTAATTACTTCGCCAACGGCGAGGCAGCTGGCATCTTTGCCtc > 2:490095/1‑142 (MQ=255) cTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCATGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTaa < 1:881558/143‑1 (MQ=255) ccACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTTCCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAAGTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAaccac < 1:935802/143‑1 (MQ=255) cacaGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAGCAGATAaccacc > 2:415751/1‑143 (MQ=255) caGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCTTGACAATGGGTGGTATTTAGAGATTCGTGAACGGCGCTAATTACGGGGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGc < 1:1204849/143‑1 (MQ=255) gTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCTAAACAGATAACCACCGCCTGGTc > 2:78641/1‑143 (MQ=255) | GCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTC > NC_000913/2661685‑2661953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |