Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,398	T→G	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	100.0%	194.0 / NA	65	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/35);  total (30/35)

TAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG  >  NC_000913/3041259‑3041533                                                                                                                                            |                                                                                                                                        tAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCATGACTCTATTATGGCTTTTACTCTTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGc                                                                                                                                      <  1:295393/143‑1 (MQ=255)  aGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAAGACTCTATTATGACTTTTACACCTATTCCTCCCCGTCAGCCTACGAATACTACATGTTTAACGCAGACACTTTATACTCTTGATCACTGGGCGCGTGcc                                                                                                                                     >  2:10244/1‑143 (MQ=255)    ctctTTTACCATTCGTCGTTAATACGCTGTCAAGCAACTCAGGTCTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccac                                                                                                                                   <  2:795809/143‑1 (MQ=255)    cactTTTGCCATTAGTCATTAATAAGCAGTTAAGCAACTAAGGACTCTATTATGGCTTTTACACATTTTCCGCACCGTCAGCCTACGGCTTCTGCCCATTTACCGCTGCCCCAGCTGACGATTGACGCCGGCGCGCGTAccac                                                                                                                                   >  2:206354/3‑143 (MQ=255)     tctTTTACCATTAGTCATTAATACTCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccacc                                                                                                                                  <  2:690960/143‑1 (MQ=255)     tctTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCAGTTTCCCGCTGACGCTAATACCGCTTGATGACTGGGCGGGTGccacc                                                                                                                                  >  2:790206/1‑143 (MQ=255)     tctTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCGTCGGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccacc                                                                                                                                  <  1:682202/143‑1 (MQ=255)       ttttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTATACTTTTTCCTCCCCGACAGCCAACGGCTTCTGCCCGTTGACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCAt                                                                                                                                <  1:259601/143‑1 (MQ=255)       tttcACCATTAGTCATTACTTTGCCGTGTAGCAACTCAGGACTCTATTCTGGCTTTTACACCTTTCCCTCCCCGTCCGCCTACGGCTTCTGCCCGTTTACCTCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCAt                                                                                                                                <  2:801350/143‑1 (MQ=255)            ccATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTTCATTTTTTTCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTg                                                                                                                           <  1:126107/143‑1 (MQ=255)            ccATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTg                                                                                                                           <  1:895371/143‑1 (MQ=255)            ccATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATGAGGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTg                                                                                                                           <  2:622498/143‑1 (MQ=255)             cATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGACATCTAACCATTTACCGCTGACGCTGATGACGCTTGAAGACTGGGCGCGTGCCACCATTACtag                                                                                                                          >  1:607765/1‑141 (MQ=255)              aTTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgc                                                                                                                         <  2:197509/143‑1 (MQ=255)              aTTAGTCATTAATACGCCGTTAAGCAACTAAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGACAGCCTACGGCTTCTGCACGTTTACCGCTGACGCCGATGCCGCTTGATGACTGGCCGCGTACCACCATAACCGgc                                                                                                                         >  2:443590/1‑143 (MQ=255)                tAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgcgc                                                                                                                       <  1:532285/143‑1 (MQ=255)                  gTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCgg                                                                                                                     >  1:333608/1‑143 (MQ=255)                     aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTGTTCCTCCCCGTCAGCCTACGGCTTCGGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACa                                                                                                                  <  1:355410/143‑1 (MQ=255)                      ttAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCTCGTCAGCTTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAg                                                                                                                 <  1:618900/143‑1 (MQ=255)                      tcaaTACTCCGTTAACCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCACGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAg                                                                                                                 <  2:265093/141‑1 (MQ=255)                         aTACGCCGTTAATCAACTCAGGTCTCTATTATGGCTTTTACATCTTTTCCTTCTCGTCAGACTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACCAGGCGCGTTCCACCATTACTGGCGCGGACAGCGa                                                                                                              <  1:1132553/143‑1 (MQ=255)                          tACGCCGTTAAGCAACTCAGTACTCTATTATGTCTTTTACATCTTTTCCTCCTCGTCAGCCTACGGCTTCTGCGCGTATACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaa                                                                                                             <  1:72491/143‑1 (MQ=255)                          tACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTCCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTTCCACCATTGCTGGCCCGGagcgcgca                                                                                                             >  2:475188/1‑136 (MQ=255)                           aCGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACAGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCGTCACTGGCACGGGCAGCGaaa                                                                                                            >  2:660636/1‑143 (MQ=255)                                       aaCTCTGGACTCTATTATGGCTTTTACACCCGTTCCTCCCCGTCAGCCTACGGCTTCTGCGCTTTTACCGCTGAAGCTGCTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAgg                                                                                                <  2:943844/143‑1 (MQ=255)                                          tCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACTGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGACAAATATATGCAGCGTc                                                                                             >  2:626738/1‑143 (MQ=255)                                             ggACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAgg                                                                                          <  1:8175/143‑1 (MQ=255)                                               aCTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGCCGCTTGATGACTGGGCGCGTGCCACAATTACTGGCGCGGACAGCGAAAAATATAAGCAGGGTCAGGTg                                                                                        >  1:739628/1‑143 (MQ=255)                                                ctctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGa                                                                                       <  2:333608/143‑1 (MQ=255)                                                      tatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCCGATGACGCTTGATGACCGGGCGCGTGCCACCGTTACTGGCGCGGACAGCGCAAAATATATGCACGGCCAGGTGAcaccag                                                                                 >  2:855117/1‑143 (MQ=255)                                                        tgtcTTTTACACCTTTTCCTTCCCGTCAGTCTACGGCTTCTGCCTGTTTACCGCTGACGCTGAGGACGCTTGAGGACTGTGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGAt                                                                               <  1:1258102/140‑1 (MQ=255)                                                        tGGCTGTTACACCTGTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTATCCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGAt                                                                               <  2:735493/143‑1 (MQ=255)                                                            ttttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgcg                                                                                                                        <  1:232425/98‑1 (MQ=255)                                                            ttttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgcg                                                                                                                        >  2:232425/1‑98 (MQ=255)                                                              ttACACCTTTTCCTCCCCGACAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGTCCCTTGATGACTTCGCGAGTACCACCATTACTGCAGAGGAAAGAGAAAAATAAATGCAAGGTAAGGTGACAGCAGATGTGAgt                                                                         >  1:171176/1‑142 (MQ=255)                                                                    cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCATGTGACACCAGATGACAGCCAAATg                                                                   >  2:629143/1‑143 (MQ=255)                                                                      tttCCTCCCCTGCGGCCTACGGCTTCTGCCCGTGTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGc                                                                 <  2:267371/143‑1 (MQ=255)                                                                             cctgTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGAt                                                          <  1:1048088/140‑1 (MQ=255)                                                                             cccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGAGTCAGGTGACAGCAGATGTCAGCCAGATAGCAGAAGAt                                                          >  2:199123/1‑143 (MQ=255)                                                                              ccGTCAGCCTACTGCTTCTGCCCGTTTACCGTTGTCGCTGAGGTCGCTTGATGATTGGGCTTGTGTCACCAGTACTGGCGCGGAGAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc                                                         <  1:486399/143‑1 (MQ=255)                                                                              ccGCCAGCGTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCTCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc                                                         <  2:1036187/143‑1 (MQ=255)                                                                                     ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGACGATCAGCagac                                                  >  2:1004597/1‑140 (MQ=255)                                                                                      cTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTg                                                 >  2:782630/1‑143 (MQ=255)                                                                                        aCGGCTTCTGCCCGTTTACCGCTGACGATGATGACGGTTGTGGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCt                                               >  2:428226/1‑143 (MQ=255)                                                                                           ccTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGTCTCGTGCCATCATTACTGGCGCGGAAAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgc                                            <  1:971536/142‑1 (MQ=255)                                                                                                tGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGTCTCCATTTCTGTCGCGGTCAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGccc                                       <  1:584072/143‑1 (MQ=255)                                                                                                  cccGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCAt                                     >  2:620325/1‑143 (MQ=255)                                                                                                        tACCGCTGTCGCTGATGACGCTTTATGATTGGCCGCGTTCCTCCTTTTCTGGCGCTGACAGCGAATAATATATGCAGGGGCAGGTCACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGAc                               <  1:326520/143‑1 (MQ=255)                                                                                                         aCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGCCCGCGAAAAATATATGCAGGGTAAAGTAACAGCAGATATCAACCAGATAGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACg                              >  1:386575/1‑143 (MQ=255)                                                                                                          ccGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGCCAGCAGATGTCAGCCAGATGGCAGAAGATCACCACCTCCTCGCCCCCCCTTGCGAcac                             >  2:211049/1‑141 (MQ=255)                                                                                                              tgacgctgaGGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAATTTTTTTCAGTGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCaaa                         <  1:472106/143‑1 (MQ=255)                                                                                                               gacgctgaTGACGCTTGATGACTGGGCGCGTGCCATCATTAGTGGCGCGGAAAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAg                        <  1:269518/143‑1 (MQ=255)                                                                                                                  gctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGTAATATATCTGCATGTTCATGTGTCAGCAAATGTCAGCAAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGACAAATGTa                     >  1:154026/1‑143 (MQ=255)                                                                                                                  gctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTa                     >  2:1120114/1‑143 (MQ=255)                                                                                                                     gatgaCGCTTGATGACTGGGCGCGTGCCACCATTCCTGGCGCGGACCGCGACAAATATATGCAAAATCAAAAAACAGAAGATGACAACCAGATGGCAGAAGATCAGCACCTGCTCACCGCCCATTGCGCCGCCAAAGGTaaca                  >  1:607651/1‑141 (MQ=255)                                                                                                                      atgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGTACAGCGAAAAATATATGCAAGGTCAGATGACAGCAGATGACAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCAAAAGGTAAAAt                 >  1:1037978/1‑143 (MQ=255)                                                                                                                       tgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg                >  2:1193730/1‑143 (MQ=255)                                                                                                                        gaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGTCAGCGACACATATATGCAGGGTCAGGTGACAAAAGATGTCAACCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtgt               >  1:705744/1‑143 (MQ=255)                                                                                                                           ggttGATGACTGGGCGCGTCCCACCATGACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGGCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGa            <  2:834115/141‑1 (MQ=255)                                                                                                                           gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGCTGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGa            >  1:358508/1‑143 (MQ=255)                                                                                                                           gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGACGATCAGCACCTGCTCGCCGCCCATTGCGCCGCCAAAGGAAAAAtgtgaa            >  1:790029/1‑141 (MQ=255)                                                                                                                           gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGa            <  2:373897/143‑1 (MQ=255)                                                                                                                             ttGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGc          <  1:620325/143‑1 (MQ=255)                                                                                                                                    cTGAGCGCGTGCCACCATTACGTGCGCGGATAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCGGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTAc   <  1:206354/143‑1 (MQ=255)                                                                                                                                     tGGGCGCGTGCCACCATTACAGGCACGGAAGGTGATATATATATGCAGGGTCAGGTGACAGCCGATGTCAGCCAGATGGCAGAAGATCAGAACCTGATAGAAGACAATAGCGACGCCAAAGGTAAAATGAGGAGCAATTTACg  >  1:227641/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                        TAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG  >  NC_000913/3041259‑3041533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑