| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 481,846 | G→A | T853I (ACC→ATC) | acrB ← | multidrug efflux pump RND permease AcrB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 481,846 | 0 | G | A | 95.8% | 106.7 / NA | 50 | T853I (ACC→ATC) | acrB | multidrug efflux pump RND permease AcrB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (21/25); minor base C (2/0); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGA > NC_000913/481706‑481985 | agaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACTATCATCTTATTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATa < 1:716068/143‑1 (MQ=255) cGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACTTCTATCTTCGTAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCa < 1:669056/143‑1 (MQ=255) gaTCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAGCACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGtt < 2:1143241/142‑1 (MQ=255) aaTCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGCTAGGCAGtt > 2:326281/1‑143 (MQ=255) tCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCAGAGAGACGATCCTGATAAGAAATCCCCGACCAGTCATAACAAACACAGATAGGCAGTTTg > 1:490763/1‑143 (MQ=255) aCCAGCTCTCGTACAGCGCAGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGAACCTGGTTGCCGGATAGACGTTCGTGATAGGACATACCCGTCCAGTAGTAGCCAACACCGCTAGGCAGTTTGCTc > 2:1165078/1‑143 (MQ=255) gCTCTCGTACAGCGCCGCCAGACACAGGAACACGTCAATCTTCGTAATTGTGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCa < 1:47462/143‑1 (MQ=255) ttcacGTACAGATCCGCTATATACATGATTACGACAATCAACGAAAACGCCGACAGGGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAg < 1:218232/139‑1 (MQ=255) aCAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGGAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCTATACGCAGATTGCTAGCCAgttgttac > 1:682676/1‑141 (MQ=255) aGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGACAGTTTGCTCGCCAATTGTTCAAt > 1:1035939/1‑143 (MQ=255) cAATCAACGAACTCGCGTACAGTGAAGGTGCCTGGTTGCCGTAGAGACTTTCCTGATAGTACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACtt < 1:155432/143‑1 (MQ=255) tCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATCGCCACCACCCATAGGCCGTTTGCTCGCCAGTTGTTCCATAAGCTCCATTTCTTAACCGGTACTTTTa > 1:61639/1‑143 (MQ=255) cgaCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATATGCAGTTTGCTCTCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAc < 2:565085/141‑1 (MQ=255) tACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTg < 1:983801/143‑1 (MQ=255) gagaGTGAAGGTGCGTGTTTGTAGTAGAGACGTGCCTGATAGGACATCCCCGTCCAGTCATAGCCTACACCGATAGGCAGTGTGCTCGCCAGTGGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTg < 2:947519/140‑1 (MQ=255) aaGGTGCCTGGTTGCCGGAGAAACGTTACTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGACAGGCCGTTTGCTCCCCAGTTGTTCAATCAGCCCCATTGCTTCACCGGTACTCTTACCCGGTGCCGCCTGGCCTagg > 2:320707/1‑141 (MQ=255) ggTGCCTGGTGGCGGGAGAGACGTGTCTGGTAGGACATCCCGGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCGTCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAt < 2:285615/143‑1 (MQ=255) gTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGCTGCCGCCTGGCCTAATAtt > 1:167313/1‑143 (MQ=255) tGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTAAACCGGTACTTTTACACGGTGCAGACTGGCCTAGGAttt > 1:179745/1‑143 (MQ=255) gCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCACCATCGCTTCACCGGTACGTTTACCCGGTGCCGCCTGGCCGAAGCTTTc > 2:1182408/1‑143 (MQ=255) ccTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTcc < 2:302052/143‑1 (MQ=255) cTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTAGCCCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCa < 1:326281/143‑1 (MQ=255) gTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCGGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatgg < 2:1251180/143‑1 (MQ=255) ttGCCGGAGAGACGTTCCTGATAGGACAGCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatgga < 2:108795/143‑1 (MQ=255) ccGGAGAGACGTTCCTGATAGGACCTCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTAAATAAACTCCATTACTTCAACGGTACTTTTACCCGGTCCCGCCTGGCCTAAGATTTCCatggatgg > 1:458749/1‑143 (MQ=255) ccGGAGAGACGTTCCTGATAGGACATCCCAGTCCAGTCATAGCCAACACCGATCGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatggatgg > 2:66305/1‑143 (MQ=255) cGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGc > 1:894974/1‑143 (MQ=255) cGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGc > 2:227340/1‑143 (MQ=255) gcagagaCTTTCCTGATAGTACATCCCCGTCCAGTCATCGCCAACACCGATATTCATTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTGCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCa < 1:542350/141‑1 (MQ=255) agaCGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGcc < 2:1181879/143‑1 (MQ=255) cGTTCCTGATGGGACATCCAAGATCAGTCAGAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGtt < 2:929484/143‑1 (MQ=255) cGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGtt < 2:894974/143‑1 (MQ=255) gTTCCTGATAGGACCTCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTg < 2:577856/143‑1 (MQ=255) ccTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCCTCAGCTCCATTTCTTCACCGGTACTTTTCCCAGGTGCCGCCTGGCCAAAGATTTCAATGGATAGCAGGCAGtcgaaa > 1:1203269/1‑138 (MQ=255) ccTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTaa < 1:227340/143‑1 (MQ=255) aTAGGACATCCCCGTCCAGTCATAGCCAACATCGATAGGTAGTTTTCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAGTTCCATGGATGGCAGGCCGTTGTAACGtt < 1:491253/143‑1 (MQ=255) cgACATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCa < 1:713138/142‑1 (MQ=255) cATCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTCGCCTAAGATTTCCGTGGATGGCTGGCCGTCGCAACGtcacagcc > 2:402459/1‑136 (MQ=255) tCCCCGTCCAGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAACATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGAcgc > 1:98254/1‑143 (MQ=255) cctttcAGTCATATCCAATTTCGATAGTCAGTTTGCTGCCCAGTTGTTTCATCAGTTCCATTGCTTCACCGGGACTGTTACCCGGTGCCGCCGGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACgcggc < 1:96502/138‑1 (MQ=255) ccGTCCAGTCATAGCCAACACCGTTAGGCTGTTTGCTTTCCAGTTGTTCTATCAGCTCCATTGCTTCACCGGTACGTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACgcggc < 1:523560/143‑1 (MQ=255) ccGTCCAGTCATAGCCAACACCGATAGGCAGTCTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGGAATTTTACCCGGTGCCGCCTGCCCTACGATTTCCATGGACGGCAGGCCGTCGCAACGTACCAGaacgagc > 2:408146/1‑137 (MQ=255) cAGTCATAGCCAACACCGTTAGGCAGTTTGCTCGCCTGTTGTTCCATCAGCTCCATTGCTGCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAAcc < 1:1165078/143‑1 (MQ=255) aGTCATAGCCAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCAATTGCTTCAGCGGTACTTTTACTCGGTGCCCCCTGGCCTAAGATACCCACTGATGACAGGCCGTGGTGACGATCCACACGCGGCGCACCg > 2:394085/1‑143 (MQ=255) aGTCATAGCCAACACCGATAGACAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGCATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCg > 2:676630/1‑143 (MQ=255) cAACACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTTCCCTGTGCCGCCTGGCCTAAGATTCCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCaa < 1:793238/143‑1 (MQ=255) aaCACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCCGCCGCGGCGAACCGTACTCCCAAc > 1:690740/1‑143 (MQ=255) aaCACCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGCACCGTACTCCCAAc > 1:849536/1‑143 (MQ=255) acCGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGAGGCGAACCGTACTCCCCACgac > 2:53262/1‑142 (MQ=255) ccGATAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACgaga < 1:66305/143‑1 (MQ=255) | AGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGA > NC_000913/481706‑481985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |