| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 94.5% | 146.9 / NA | 56 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base C (25/27); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT > NC_000913/2132652‑2132925 | tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTTTTTGTTTTAGTACCCGGAGAATGTGTTCGGTGACGAGGGGAGCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt < 1:63091/143‑1 (MQ=255) ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACAATAATTGATATACAGGCTCTACGTc > 1:545640/1‑143 (MQ=255) tAGAGGGTAAACAGCTGATATTTTTTCTTGGAGCGGTTTAATTTTTCTTTGTGCTTGCTGTAAAAGTGGAAGTACCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 1:216212/143‑1 (MQ=255) tAGAGGCTAAACAGCTGATATTTTTTGCTGGCGCGGTCTAATTTGTTTTTGTGCTTGCGGTTAAAGTGTAAGTACCCGTAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 1:585622/143‑1 (MQ=255) gagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAGAGTGGAAGTACCCGGAGGAGTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCct < 2:483016/143‑1 (MQ=255) gTAAACAGCTTATATTTTTTGCTGTCGTTGTTGTTTTTGTCTTTTTGCTTGTGGTAAAAGTGGAAGTACCCGGAGAATTTTGTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc < 1:1066256/143‑1 (MQ=255) gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTGAACGAAAAAAAAATGCAGGCTCTGCGACGCCtcttc > 1:503196/1‑143 (MQ=255) gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc > 1:539990/1‑143 (MQ=255) aCAGCTGCTATGTTTTGCTGGCGTGGTCGAATTTGTCTTTGTGCGTGCGGTATTAGGTGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACt < 2:1001765/143‑1 (MQ=255) aCAGCTGATCTTTTTTGCTGGCTCGGTCGAATTTGTCTTTTTGCTTGCGGTATTTTTGGTAGTACCCGGAGAATTTGTTCGGTGACGAGGGGATCGGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACt < 1:50487/143‑1 (MQ=255) aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCTGTGACGATGTTATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCTCTGCTTCTCCttt > 1:628638/1‑143 (MQ=255) gCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc < 1:415309/143‑1 (MQ=255) ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAACTTTTTCGATGACGAGGTGATCTACATTTCACCGCGATTGATATGTAGGATCAGCCCCGCCTCTTCTACTTTCCACGGTTCg > 1:1187596/1‑143 (MQ=255) tGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCAACGTGATTGATATGCAGGATCTGCGTCGCCTATTCTACTTTCCACGGTTCGCCg > 1:56503/1‑143 (MQ=255) tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGCTATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTCCGCCGtca > 2:221219/1‑141 (MQ=255) cgcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc < 1:1001116/143‑1 (MQ=255) gcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGTAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcca > 1:803915/1‑143 (MQ=255) tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTccaccac > 2:578485/1‑143 (MQ=255) aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGTCGCGGTGATCTGCATTTCACCGTGATTGATATACAAGATCTGCGTCACCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc > 1:40584/1‑143 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTAGACGTACTTCACCATCATCCGccaga > 2:292678/1‑139 (MQ=255) ttGTGCTTGCGGTAAAAGTAGAAGTACCCGGAGAATTTTTTCGGTGTCGTGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:101872/143‑1 (MQ=255) gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa > 2:1038563/1‑143 (MQ=255) tgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCTTTTTACCGTGATTGATATGCAGGCTCTGCGTCGCCGCTTCGACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAt < 1:463452/143‑1 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc < 1:432549/127‑1 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc > 2:432549/1‑127 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGACGt > 2:586624/1‑143 (MQ=255) tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCTCTCCCCAGGAACATGTCGTAATc > 2:233953/1‑143 (MQ=255) aGTGTAAGTACCCTGAGAATTTTTTCGGTGACGAGGTGTTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt < 1:539007/143‑1 (MQ=255) aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt < 2:1185368/143‑1 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTTTGc > 2:1214653/1‑143 (MQ=255) aaGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc > 2:212210/1‑143 (MQ=255) gTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa > 2:370974/1‑120 (MQ=255) gTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:370974/120‑1 (MQ=255) ttcccGGAGAGTTTTGTCGGTTACGGCGTGACCTGCATTTCACCGTGTTTGATATGCGGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt < 2:1247012/141‑1 (MQ=255) ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGGTGTCGTGATCCTGTGCCGCTTTCAGtt > 1:1209123/1‑143 (MQ=255) ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt > 1:934785/1‑143 (MQ=255) ggAGAATTTTTGCGGTGCCGCTGTTATCTGCATTTCACCGTGTTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt < 1:498452/143‑1 (MQ=255) gtgaATTTTTTCGGTTACGATGTGATTTTCATTTCTCTGTGATTGATATTCTGGTTCTGCGTCGCCACTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc < 1:151107/141‑1 (MQ=255) gagtttgttCGGTGACTAGGTGATCTGTATTTCATCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACGTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCgg < 1:766267/140‑1 (MQ=255) ttttttCGGTGACGAGGTGATTTGCATTTTACCTTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt < 1:540009/143‑1 (MQ=255) gttttCGGTGCCGAGGTGATCGGCATTTCCCCGTGATTGAGATGCAGGCTCTGCGTCGCGTCTTCGACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGAAGATGTCGTAATCCTTTGCCGCTTTCAGTTCGGTATc < 2:933915/142‑1 (MQ=255) ttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCAACGGATCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATGCTGTGCCGCTTTCAGTTCGGTATCGaa > 1:1044739/1‑143 (MQ=255) ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGTGTTGCCTCTTCTACTTTTCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa < 1:933980/143‑1 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 2:539990/143‑1 (MQ=255) gACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCTCCGTACTCCACCACCATCTGAAGGAAGATGTAGTAATCCTGTAAAGCAATCAGTTAGGTAGCGaacaaaca > 1:170963/1‑143 (MQ=255) gACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGACGTCGTAATCCTGTGCCGCTTTCAGTTCGATATCGaacacacc > 1:1243118/1‑142 (MQ=255) gatgTGATCTGCATTTCACCGTTATTGATATGCAGGCTCTGTGTCGTCTTTTCTTCTTTCTACGGTTCGCCGTGCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActc < 1:923788/140‑1 (MQ=255) gTGCTCTGCAGTTCACCGTCATTACTATTCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt < 2:1152715/143‑1 (MQ=255) gTGATCTGCATTTCTACATGATTGAGATGCAGGCGCGGCGGGGCGTGTTGTAGTGGCAGCGGGTCGCCGGACTCGACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt < 1:185871/143‑1 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCATTCAGTTCGGAATCGAACAAACACTAttt > 1:129890/1‑143 (MQ=255) ctggtCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCTGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt < 2:722021/139‑1 (MQ=255) tGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACAGTGCGCCGTACTCCACCACCATCCGCAGGAAGACGTCGTACTCCTGTGCCGCTTTCAGTTCGGTATCGCACAAACCCTCTTTg > 2:555094/1‑143 (MQ=255) tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTGTTTGaaaag > 1:547825/1‑141 (MQ=255) gCATTTCACCGTGATTGATATGCAGGCTCTGCGTAGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTCCCGCTTTCAGTTCGGTATCGAACAAACACCATTTGAAAcac > 2:44045/1‑141 (MQ=255) ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTTCTTTCCACGGTTCGCCGTACTCCATCATCTTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCa < 1:571000/143‑1 (MQ=255) cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACGCTTTAAAACGCCATGCCCAGGCAAAGACCAGGt > 2:1173228/1‑143 (MQ=255) | TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT > NC_000913/2132652‑2132925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |