| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,173,361 | Δ2 bp | 100% | pseudogene (917‑918/1358 nt) | gatC ← | galactitol‑specific PTS enzyme IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,173,361 | 0 | C | . | 94.6% | 145.3 / 0.3 | 37 | pseudogene (918/1358 nt) | gatC | galactitol‑specific PTS enzyme IIC component |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base . (22/13); total (22/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
| * | NC_000913 | 2,173,362 | 0 | C | . | 92.0% | 136.0 / 1.5 | 37 | pseudogene (917/1358 nt) | gatC | galactitol‑specific PTS enzyme IIC component |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base . (22/12); total (22/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAA > NC_000913/2173220‑2173488 || gATTTCCACGATGCACGGTGACCGCCATCGCAACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGTCACACAAACAGCAATTAAAATGGTGAGTGGGATAATTTTCAGTGTTGCCGCGaccacc < 2:184785/143‑1 (MQ=255) cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccgccgccgta < 2:314812/143‑7 (MQ=255) gCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGGATGTccc > 2:147088/1‑143 (MQ=255) cgcGGCGACCGCCATCTCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAACGGTGATTTGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCca < 2:416403/141‑1 (MQ=255) cACGGCGACCGCCATCGCCACGTAGAAGCCGATGGGGGCAAGATCGCCAAACGGCAGCATCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTcaca < 2:352110/143‑1 (MQ=255) ggCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCcagca > 1:531970/1‑143 (MQ=255) cGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGTCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCaaag > 1:432966/1‑141 (MQ=255) gCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTACAATGGTGAGTGGGATCAAAATCAGGCTTGCCGATACCA‑‑CCGCCGAATGTACCAGCAGCAACGc > 1:367766/1‑143 (MQ=255) gCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGGGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGc < 1:398243/143‑1 (MQ=255) aTCGGCAGGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCgg < 1:309076/143‑1 (MQ=255) agaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCa < 1:180879/143‑1 (MQ=255) aagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAt > 1:26180/1‑143 (MQ=255) gCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCCCACAAACAGCAAATAAAATGATGAGTAGGATAAAAATCAGGCTTACCAATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCa > 1:97077/1‑143 (MQ=255) ccGATTCTGGCAACATCGCCAAACGCTACCGTCTGATTACCCTGCACACGAACAGCAATTAAAATGGTGAGTGTGATAAAAATCAGGCTTGCCGATACCA‑‑CCTCCGTATGTCCCAGCTGCAACGCCGGATCAAGGCCAATCAg < 2:705342/143‑1 (MQ=255) ggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAACATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACt > 2:766051/1‑143 (MQ=255) aaGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGTTCCAGGCCAGTCCGTCACTCCTgtc > 1:61551/1‑141 (MQ=255) aTCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGTTACCA‑‑CCGCCGTAAGGCCCAGCAGCCACGCCGGGTCACGGCCATTCAGGAACTCGTGAccgc > 1:317685/1‑143 (MQ=255) aTCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAACAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc > 1:448967/1‑143 (MQ=255) cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCACCGCCGGATCAAGGCCAATCAGGCACTCCGCACCGCCGAAc > 2:743409/1‑143 (MQ=255) ggCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCACGAACTCCTGACCGCCGAACATCGc > 2:392125/1‑143 (MQ=255) gccagctCCTGATTACCCGGCCCACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGAT‑CCA‑ACGGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc < 2:367766/141‑1 (MQ=255) cgcagcaCCTGCTTACCCGGCACACAAACAGCAAGTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc < 2:102557/142‑1 (MQ=255) agcaCCTGATTACCCGGCCCACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTg < 2:477424/143‑1 (MQ=255) atcaCCCGGCACACAAAAAGCAATTATTATTTGGTGTGGCATCAAAATCAGGCATGCCGATACCA‑‑CCGCCGTCTGTCCAACAAGCAACGGCGGATCAACGCCAATCAGGAACTCCTGACCGCCGAGCTCCGCCTGAAAACGAc > 2:673776/4‑143 (MQ=255) aCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAAAGACTAc > 2:664153/1‑143 (MQ=255) cGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCGGATACCA‑‑CCGCCGTATGGCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGGACTCCTGACCGCCGACCTTGGCCTGTCAACGACTACggg > 1:124986/1‑141 (MQ=255) acaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCACTCAGGAACTCCTGACCGCCGAACTTCGCCTGCACACGACTACGAGCCTGcc > 1:741892/1‑142 (MQ=255) gaaaCAGCAATTAAGATGGTGCGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCtt < 2:250820/142‑1 (MQ=255) aaaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGt < 1:692272/143‑1 (MQ=255) aaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCATGTAAACGACTACGAGCCTGCTTAGCGATGGGCGtt > 2:370646/1‑143 (MQ=255) aaaTGGTGAGTGGGATAAAAATCAGGCTTACCGATACCA‑‑CCGCAGTATGTCCTAGCAGCAAAGCCGGATCAAGGCCAATCAGGAACACCTGACCGCCGAACTTCGCCTGTAAACGACTACGAACCTGATCAGCAATAGGCGtt > 2:485077/1‑143 (MQ=255) aaaGGGTGAGTGGGATAAAAACCAGGCTTGCCGAGAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGtt < 1:286259/143‑1 (MQ=255) aaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCGAATCAGGAACTCCTGACCGCCGAACTTCGCGTGTAAACGACTACGAGCCTGCTTGGCGCTGGGCGTTa > 1:70393/1‑143 (MQ=255) gAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCGAGCAGCCACGCCGGATCAAGGCCAATCAGGAACTCCTGACCCCCGAACTTCGCCTGTACACGACCACGCGCCTGCGTAAGCATAGGACTTAAAccat > 2:244268/1‑143 (MQ=255) tAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgctg > 1:225104/1‑143 (MQ=255) gaatctCAGGCCTGCCGATACCA‑‑CCGCCGTAGGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGCAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATgg < 2:146823/138‑1 (MQ=255) aaacagcAGGCGTGCCGATACCA‑‑GCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACGCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATgg < 1:296475/137‑1 (MQ=255) aaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTTGCGATGGGCGTTCACCCATCCATAATGTGtt > 1:90654/1‑143 (MQ=255) cAGGCTTGCCGATAC‑‑CCCCGCCGTATGGCCCGGCAGCATCGCCGGATCAACGCCAATCAGGAACTCCTGACCGCCGAACTTCGCTTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa < 2:34232/143‑1 (MQ=255) || GATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAA > NC_000913/2173220‑2173488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |