| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 639,685 | Δ1 bp | 14.7% | intergenic (+177/‑68) | ahpC → / → ahpF | alkyl hydroperoxide reductase, AhpC component/alkyl hydroperoxide reductase, AhpF component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 639,685 | 0 | A | . | 14.7% | 75.0 / 12.1 | 34 | intergenic (+177/‑68) | ahpC/ahpF | alkyl hydroperoxide reductase, AhpC component/alkyl hydroperoxide reductase, AhpF component |
| Reads supporting (aligned to +/- strand): ref base A (16/12); new base . (3/2); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.16e-01 | |||||||||||
GTCGCCCGCTCACCCCGGTCACTTACTTGTGTAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAACTCAAGGCTTACCTTGAGAAATTGACCAAGCCTGTTGAGTTAATTGCC > NC_000913/639544‑639827 | gTCGCCCGCTCACCCCGGTCACTTACTTGTGTAAGCTCCCTGTGATTCTCAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCatgat < 2:409097/143‑1 (MQ=255) cacccACTTACTTGTGTAGGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAg < 1:342999/139‑1 (MQ=255) cccttccttGTGTAGGCTCCCGGGGATTCGCAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAg < 2:629602/141‑1 (MQ=255) ttacttGTGTAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGCTTTTCTTCCCCGCCCCATGATGCAAGCTGCATCCAGGTAGCCg > 2:141962/1‑143 (MQ=255) ttGTGTAAGCTCCCGTGGTTTCTCAGGCTATCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCaga < 1:17170/143‑1 (MQ=255) gtgtAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGCTGCTGCGGGAACCGATAGCCGTCCCCGCCCCATGATCCAAGCTGCATCCAGATAGCCACaaagt > 1:206133/1‑142 (MQ=255) gtAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGcc > 2:695304/1‑143 (MQ=255) ccGGGGATTCACAGGCTAGCCGCATTGCTCTGACGCGAAATACTTCGGAAATGCACCTAACTCATCGGGTGCTGCGGCACCCGATTTTCTTTCCCGCACCATCATGCAAGCTGCACCCCGCTAGCCCCCGCAGCCGCCTGCat > 2:237654/1‑143 (MQ=255) cGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGCGCTGCGGCACCCGATTTTCTTCGCCGCACCATGATGCACGCTGCATCCAGGTAGCCGCAGAGACGGCGTGCatg > 2:762228/1‑143 (MQ=255) tCACAGGCTAGCCGCCTTGCTCTGACGCGAAATAATTCGGAAATTAACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCGCGGCACCAGGATGGAAGCTGCAGCCAGGTAGCCGCAAAGGACACCTGCaggatgaagc > 2:482518/1‑140 (MQ=255) acaGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCCGAGGCCGCTTTCATGATGATGttt > 1:154434/1‑143 (MQ=255) caGGCTAGCGGCCTTGCTCTGACGCGAAATACTTCGGAAATCCACCTAATTCTTCGGGTGCTGCGGCGCCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTa < 1:700749/143‑1 (MQ=255) ccgccTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATGTTCTTCCCCGCACCATGGTGCAAGCTGCAGCACGGTAGCCGCAGGCGCCGCGGGCATGAGGAAGCATAAAgagaag > 2:281662/1‑138 (MQ=255) ccgccTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGCGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCACAGAGGCCGCTTGCATGATGATGTTTGAAGCCCAg > 2:656343/1‑143 (MQ=255) ttGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCCCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGAt > 2:470636/1‑143 (MQ=255) tGCTCTGGCGCGAAATACTTCGGAATTTCACCTAATTCTTCTGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATa < 2:422563/143‑1 (MQ=255) gCTCTGTCGCGAAATACTTCTTATATTCACTTAATTCTTCGGGTGCTGCGGCACCCGATTTGCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATaa < 1:194628/143‑1 (MQ=255) atctGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATG‑TGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAAc > 2:307920/2‑143 (MQ=255) tctGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCGTGATGATGTTTCAAGCCCAGGAGCTAAAc > 1:455793/1‑143 (MQ=255) tctGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCGGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAAc > 1:302096/1‑143 (MQ=255) tGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATG‑TGCAAGCTGCATCCAGGTAGCCGCAGATGCCGCTTGCATGATGAAGTTTAAAGCCTAGAAAATAACCATg > 1:70121/1‑143 (MQ=255) gAAATACTTCGGAAATTCGCCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGa < 1:371262/143‑1 (MQ=255) tACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATG‑TGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACaa < 2:531699/143‑1 (MQ=255) ttCGGAAATTCACCTAATTCTTCGGGTGCTGCGACACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGCTTAAAGCCCAGGCGATAAACCTGCTCGACACaaag > 2:61157/1‑142 (MQ=255) gAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATG‑TGCAAGCTGCATCCAGTTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGaa < 2:278695/143‑1 (MQ=255) cACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAaactc > 2:588346/1‑143 (MQ=255) cTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATG‑TGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCCCGACACAAATATGAAaactcaact > 1:369679/1‑143 (MQ=255) cttcTGCTGCTGCGGCACCCGATTTTCTCCCCCGCACCATGATGCAAGCTGCATCCAGCTAGCCGCAGAGGCAGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAACTCAAgg < 2:236516/143‑1 (MQ=255) cttcGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAACTCAAgg < 1:660441/143‑1 (MQ=255) gCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAACTCAAGGCTTACCTTGAGaaa < 1:652811/143‑1 (MQ=255) ccGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCAGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATCCTCGACACCAATATGAAAACTCAACTCAAAGATTCCCTTGAGaagatgc > 2:80896/1‑138 (MQ=255) tttCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGCTGTTTAAATCCCCGGAGATAAACATTCTCGACACAAATATGAAAAATCAACTAAAGGCTTACCTTAAGAAATTGACCaaa > 1:207651/1‑142 (MQ=255) ttcttcCCCGCACCAGGATGCAAGCTGCATCCAGGTAGCCGCAGAGCCTGACCTTACAATTATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAACTCAAGGCTTACCTTGAGAAATTGACCAAGc < 1:765015/143‑1 (MQ=255) ccATGATGCAAGCTGCATTCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAACTCAAGGCTTACCTTGAGAAATTGACCAAGCCTGTTGAGTTaa < 1:58627/143‑1 (MQ=255) ccATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAACTCAAAGCTTACGTTGAGAAATTGACCAAGCCAGTTGAGTTaa > 2:688142/1‑143 (MQ=255) gttgatgCAAGGTGGATACAGGTAGCCGCAGACGCCGCTTGCATGACGATGTTTAAAGCCCAGGAGATAACCATGCTCGACCCAAATATGAAAACTCAACTCAAGGCTTACCTTGAGAAATTGACCAAGCCTGTTGAGTTAAt < 2:68878/141‑1 (MQ=255) atgatgCAAGCTGCATCCAGGTAGCCGCAGAGTCCGCTTGCATGATGATGTTTAAATCTCAGTAGATAAACATGCTCGACACAAATATGAAAACTCAACTCAAGGCTTACCTTGAGAAATTGACCAAGCCTGTTGAGTTAAtt < 1:187668/143‑1 (MQ=255) atgCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAACTCAAGGCTTACCTTGAGAAATTGACCAAGCCTGATGAGTTAACTGcc > 2:721644/1‑143 (MQ=255) | GTCGCCCGCTCACCCCGGTCACTTACTTGTGTAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAACTCAAGGCTTACCTTGAGAAATTGACCAAGCCTGTTGAGTTAATTGCC > NC_000913/639544‑639827 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |