| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,637,645:1 | +T | 100% | coding (4/1500 nt) | pitA → | metal phosphate:H(+) symporter PitA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,637,645 | 1 | . | T | 92.6% | 69.5 / 0.2 | 27 | coding (4/1500 nt) | pitA | metal phosphate:H(+) symporter PitA |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (10/15); total (10/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑C‑TACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGC > NC_000913/3637507‑3637779 | tatataAGATTATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑C‑ttac < 1:369627/143‑4 (MQ=255) taAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACATTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACAttt > 1:106678/1‑143 (MQ=255) tattctGACTGAAACCCATACACGAGCCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGcc < 2:307410/138‑1 (MQ=255) tttttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCt < 1:448516/143‑1 (MQ=255) tttgGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATTTAGCATGCAACCCTCGCCGCGACGGGTGCTGATAATGCGCCGCGTTCATGTCCTCAAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCt < 2:336284/143‑1 (MQ=255) gAGTGACATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAAGGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGAtt < 1:295651/143‑1 (MQ=255) tGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTCGGTTGCTGGCCTGGATTTGc > 1:481943/1‑143 (MQ=255) aCAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTGACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCt < 2:334290/143‑1 (MQ=255) gggtaaaaTAAAAAATAGTCTGTATTTCACTTTGCCCGCGCCGCGAAAGTCACTTATAATGCGTCGCGTTTATGTCCTC‑AAAATGGTGGAATGTCCTATGTT‑TACATTTGTTTGCTGGCCTGTATTTTCATAACGGGCTGtta < 2:676111/138‑1 (MQ=255) gggCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATTCCGGGCTGttat > 1:39843/1‑143 (MQ=255) cTTTGCCCGAGCCGCGAAAGTCATTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGtt < 1:516497/143‑1 (MQ=255) cTTGGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGtt < 1:142253/143‑1 (MQ=255) cgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGc < 2:601577/143‑1 (MQ=255) cgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCAt < 2:39843/143‑1 (MQ=255) ggcACTGATAATGCGCTGCGTTCATGTCCGC‑AGTATGGCGTAACGTCCGATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGt < 2:94764/141‑1 (MQ=255) tCACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGtt > 1:690908/1‑143 (MQ=255) tAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa < 2:381069/143‑1 (MQ=255) tAAGGCGCCGCGTTCATGTGCAC‑AAAATGGCGTAACGTCCGATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa < 1:405249/143‑1 (MQ=255) gcgcCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGCTTTGCATACCGGGCTGTTATTATTGCTTGCACTTGCTTTTATGCTGTTCTAAGAAGACATCAATGGTTTCGAGGAcaca > 1:405356/1‑143 (MQ=255) cggggcgTTCATGTCCTC‑AGAGTGGCGGAACGTCGTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAg < 1:718255/139‑1 (MQ=255) gcgTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCaa > 1:671234/1‑143 (MQ=255) cATGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTGATTGTTGCTTGTACTGGCTTATTTGCTGTTCTACGAAACCCTCAATGGTTTCCACTAACCAGCCAAcacag > 2:86487/1‑139 (MQ=255) aTGTCCTC‑AAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTGTCCATGACACAGCCAACGcctt > 2:646680/1‑141 (MQ=255) ctc‑tAATTGGTGTTACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCaa < 1:322470/143‑1 (MQ=255) cggCGTAACTCCCTATG‑CTTACATCTGCTTGCTGGCCTCGATTTGCATACCGGACTGTTATTACTGCTTGCACTGGCTTTTGTGCTGTTCTACCAAGCCAGCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTAt < 2:4031/142‑1 (MQ=255) gCGTAACGTCCTAAGCC‑AACAATGGGTGGCGGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCt < 1:319142/143‑1 (MQ=255) gTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATTACACAGCCAACGCCGTGGCAACCGTTATCTATACCcg > 1:544743/1‑143 (MQ=255) tCCTATG‑CTTACATTTGTTTGCTGGCCGGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCGTTTGTGCTGTTCTGCGAAGCCATCAATGGTTTCCATGACACAGCGAACGCCGTGGCAACAGTTAGCGCTACCcgg > 1:400451/1‑142 (MQ=255) | TATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTC‑AAAATGGCGTAACGTCCTATG‑C‑TACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGC > NC_000913/3637507‑3637779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |