Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	2,399,711	Δ123 bp	11.6%	coding (333‑455/2727 nt)	nuoG ←	NADH:quinone oxidoreductase subunit G

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2399710	22 (0.590)	3 (0.080)	3/262	NT	11.6%	coding (456/2727 nt)	nuoG	NADH:quinone oxidoreductase subunit G
	?	NC_000913	2399834 =	24 (0.650)					coding (332/2727 nt)	nuoG	NADH:quinone oxidoreductase subunit G

TTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2399568‑2399710 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAAC  >  NC_000913/2399834‑2399908                                                                                                                                                                                                                            TTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTACGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTCTACCGTCAGCGTAATCTATGTAGTAACGCACACAGCGTTAGCAGGCG                                                                             >  2:46615/1‑143                CGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGAGGAAGCAGGCGTTACCGACCTCTTCA                                                              >  1:548139/1‑143                                                  GCGACCGAAGTAGACGTTGGCGTGCGCACCGGAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCA                            <  1:262586/143‑1                                                                            CACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAAC  >  1:327420/1‑143                                                                                                                                                                                                                            TTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2399568‑2399710 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAAC  >  NC_000913/2399834‑2399908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.