| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,111,164 | G→T | 32.1% | R101L (CGT→CTT) | opgH → | osmoregulated periplasmic glucans (OPGs) biosynthesis protein H |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,111,164 | 0 | G | T | 32.1% | 38.3 / 13.2 | 33 | R101L (CGT→CTT) | opgH | osmoregulated periplasmic glucans (OPGs) biosynthesis protein H |
| Reads supporting (aligned to +/- strand): ref base G (9/13); new base T (6/5); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCA > NC_000913/1111028‑1111305 | gAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGGGAGCGGCTGAAGGCGCTGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCTTTTCTg > 1:318473/1‑143 (MQ=255) aaCAAGCCTGGTCAGATTCATTTTCTTATGTATAGTTAATTAAAGACGACGAAGGGCGCGAGCAGCGGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTgg < 1:706538/143‑1 (MQ=255) ggtcggATTCACCGGCTGATGGACAGTTAATTAAAGACGACGAAGTGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTg < 2:389641/138‑1 (MQ=255) cAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGACAGAAGCAAAACGCTCCTCGATGTTTCCCGACCGGTGGCGGACCAACCCGGTAGGGCGGTGCTGAGATCGGCtctgc > 1:636639/1‑139 (MQ=255) gATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTgg > 2:583212/1‑143 (MQ=255) aTTCACTTGCTGATGGACAGTTAATTATATACGACGTAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGAGGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGa < 1:211587/143‑1 (MQ=255) gATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTAGGATCGCCTGCGTGGACACGATGTCAc > 1:116458/1‑143 (MQ=255) tGGACAGTTAATTAAAGACGACGAAGGGCGTGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCAcgc < 1:629754/143‑1 (MQ=255) gACAGTTATTTAATGTCGACGAATGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCAcgccg < 1:393065/143‑1 (MQ=255) aCAGTTATTTAAAGACGACGAAGGGCGCGATCAGCCGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCAcgccgc < 1:248723/143‑1 (MQ=255) gTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCCGCGGGGACGCGATGCCACGCCGCGCt > 2:653170/1‑143 (MQ=255) aattaaAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATc > 2:194338/1‑143 (MQ=255) ttaaAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTg > 1:523056/1‑143 (MQ=255) gacgacGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCCCGCTCTCTGGCTcc > 2:386085/1‑142 (MQ=255) gaAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAACCGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGTTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGAc < 2:680125/143‑1 (MQ=255) ggCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGAGGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCTTTTCTGGGGTCGCCTGCGTGGACGCGATGTGACGCCGCGCTATCGGGCTGGTTTGaagcaa > 1:299194/1‑138 (MQ=255) cgATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCACCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGGTTGACCGaagaag > 1:109162/1‑143 (MQ=255) tCAGCTGAAGGCGATGCCAGAAGCAAATCGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGc < 1:612882/143‑1 (MQ=255) agAAGGCGATGCCAGAAGCAAAACGCTGCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCCAAGAAGAGCAGgag > 1:483745/2‑143 (MQ=255) gAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGCCGTCACGCCGCGCTATCTGACTCGTTTCACCACAGAAGAGccagaca > 1:734954/1‑137 (MQ=255) gAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGgaga < 1:451970/143‑1 (MQ=255) agagGCAAAACGCTCCTGGTTGTTTCCCGACCCGTGGCGTGCCAACCCGGTAGGCCGTTTGTGGGATTGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGt < 2:394676/143‑1 (MQ=255) aGCAAAACGCACACAGAAGTATCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGc < 1:166533/143‑1 (MQ=255) ctcGATGGTTCCCGACCCGTGGCGTACCATCCCGGTAGGCCGTTTCTGGGATCGCTTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTGTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTa < 1:361239/143‑1 (MQ=255) gTTTCCCGCCCCGTGGCGTACCAACCCGGTAGGCTGTTTCTGTGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCGGGCTCGTGAGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATcc < 1:578856/143‑1 (MQ=255) tttCCCGACCCGTGGCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCACAGAAGAACAAGAAAACGAGCAAAAGTGGCGTACCGTCGGTACAACccg > 1:21544/1‑143 (MQ=255) ttgacgtcccGTGGCGTACCATCCGGGTACGCGGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATccgc < 2:44954/136‑1 (MQ=255) tCCCGACCCGTGGCGTACCAACCCGGTAGGCTGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTCTCTGGCTCGTTTCACCAAAGAAGAGCCGGAGACCCAGCAAAAGTGTCGTACAGCCACTCCCATccgcc > 2:581932/1‑143 (MQ=255) cccaCCCGTGGCCTACCAACGCGTTAGGCGTTTTCTTGGATGGCGTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGt < 1:622357/140‑1 (MQ=255) cccGTGGCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACa < 1:583212/143‑1 (MQ=255) aCCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGAt < 1:707462/143‑1 (MQ=255) ccggggaGGCCGTTTCTGGGACCGCCTGCGGGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGa < 1:487724/137‑1 (MQ=255) cGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGCGCAGCAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCa > 1:121906/1‑143 (MQ=255) | GAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTGGCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCA > NC_000913/1111028‑1111305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |