| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 100.0% | 105.5 / ‑6.7 | 39 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (17/21); total (18/21) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAAC‑AAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTG > NC_000913/2132647‑2132923 | ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAACTCTTTCGGTGACGAGGTGATCCGCATTCCACCGTGATTGATATGCAGGCTc > 2:725988/1‑143 (MQ=255) aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTg > 2:553482/1‑143 (MQ=255) tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGATTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATCGATATGCAGGctctcc > 2:136778/1‑141 (MQ=255) agagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGATTTATCGCCTTCACCAGAAAATCTCCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc > 1:557868/1‑143 (MQ=255) agagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc < 1:462837/143‑1 (MQ=255) aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAc < 1:714979/143‑1 (MQ=255) aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTACATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACt > 1:372956/1‑143 (MQ=255) aGCTGCTATTCTTTGTTGGCGTGGTCGACCTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttt < 2:617664/143‑1 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCGGGAGAATTTTGTCGGTGACGAGGGGATATGCATTTCAACGTGATTGATCTGCAGGATCTGCGTCGACTATTCTACTTGAGACGGtt > 2:589230/1‑143 (MQ=255) ttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGAGGAAACACCCGGTGCCGTTTTTCGATGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc < 1:476550/143‑1 (MQ=255) ttGCTGGCGCGGTCGAATTTGTCTGTGTGCTTGCGGTAAAAGTGGAAGGACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc < 2:737903/143‑1 (MQ=255) ggTCGAATTTGTCTTTGTGCGTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc < 1:243404/143‑1 (MQ=255) gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGACGCATAATCTACTTTCCACGATTCGCAATACAaaaccacca > 1:501031/1‑143 (MQ=255) ggcTTTGTGTTGGCGGTAAGAGTGGAAGGACCCGGAGGAGTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa < 2:493434/141‑1 (MQ=255) gTCTTTGTGCGGGTGGTGAAGGTGGAAGTGGATGGTGGGTTTTGTTGCTGGTGAGGTGATCTTCATTTGCCCGTGATTGATATGCGGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAGTCCACCACCATCCGCa < 2:592651/143‑1 (MQ=255) ttGTTCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:669690/143‑1 (MQ=255) gtgCTTGCGGTTAATGTGGAAGTTCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa < 1:569761/143‑1 (MQ=255) agcgtGCGGTAAAAGTGGAAGTACCCGGAGAGTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAt < 1:734721/139‑1 (MQ=255) ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTCTGCGTAGCCTCTTCTACTTTCAACGGTGCGCCGTAGTCCACCACCATCCGCAGGAAGAGGTCgaca > 2:631285/1‑140 (MQ=255) ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGACGTaa > 1:692762/1‑143 (MQ=255) aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGTTCCGCCATACTCCACCACCCTCCGCCTGACGATGTCgccctcct > 2:713296/1‑136 (MQ=255) aactgtaaGTACCCGGTGTATTTTTTCGGTGATGAGCTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTTCGTCGCCTCTTCTACTTTCCACTGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtg < 2:234877/137‑1 (MQ=255) ttGGAAGTACTCGGAGTATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg < 1:618328/142‑1 (MQ=255) gTGGAGGTACCCGGAGAATTTTTTCGGCGACGATCTTATTTTCTTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg < 1:725988/143‑1 (MQ=255) tGGAAGTGCCCGTGGCATTTTTTCGGTGACGCGTTGTTCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGCTTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCTTCCGCAGGAAGATGTCGTAATCCTGTGc < 2:537755/143‑1 (MQ=255) gAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCACCAAGAAGAAGACGTAACCATGTACCg > 1:419755/1‑143 (MQ=255) gAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTACTCATGTGccc > 2:519343/1‑142 (MQ=255) cccGTAGTATTTTCTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa < 2:110982/143‑1 (MQ=255) cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa < 1:423976/143‑1 (MQ=255) ggaactgttttgCGTTGATGTGGTTATCTGCACTTCACCGTGATTGATATGCAGGCTCTGCTTCGCGTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt < 2:238069/136‑1 (MQ=255) ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACCCGGTCGCAACCCTGTGCCGCATTCAGGTCGGtta > 2:78748/1‑141 (MQ=255) cGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATTCTGTGCCGCTTTCAGATCAGTAACGAAA‑AAACAct > 1:436499/1‑143 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATCTCGTAATCCTCTGTCGCTATCAGTTCGCTATCGAACTAAacacattt > 2:915/1‑139 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAAGTCGTAACCCTGTGCCGCTCTCAGTTCGGTATCGAAC‑AAACACTCTTCGac > 2:550988/1‑142 (MQ=255) tccgCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCTCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAAC‑AAACACTCTTTGaaa < 2:137891/140‑1 (MQ=255) cATTTCGCCGTGATTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAAC‑AAACACTCTTTGAAACGcc < 1:136778/143‑1 (MQ=255) aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCTACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCCGTTCAGTATCGAAC‑AGACACTCTTTGCAACCCGATGccg > 1:513361/1‑142 (MQ=255) gTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATATAGTAATTCAGTGACACTTTCAGTTCGGTATCGAAC‑AACCACTCTTTGAAACGCCATGCCCAgg > 1:381923/1‑143 (MQ=255) gttggtATGCAGGCTTTGTGTCGCCCCTGCTACTTTCCGCGGTTCGCCTTACTCCACCGCCATCCGCAGGAACATGTCTTAGTCGTTTGCCGCTTTCAGTTCGGTATCGAAC‑AAACACTCTTTGAAACGCCATGCCCAGGTaa < 2:326684/142‑1 (MQ=255) tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCAGGCAGCTGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAAC‑AAACACTCTTTGAAACGCCGTGCCCCGGCCAAGCCCTg > 2:141438/1‑143 (MQ=255) | GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAAC‑AAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTG > NC_000913/2132647‑2132923 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |