| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | 100% | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 100.0% | 87.3 / ‑6.7 | 39 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (18/19); minor base G (0/1); total (19/20) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGT > NC_000913/125254‑125521 | ggCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTCTATCGCATAGGtaatga > 1:451254/1‑143 (MQ=255) tttgTTCTCATGTTGCTAGCGTGAGCTTCCTCTCCGAGTTTGCTGGTGATGGTCAGGGTGGTGAATGCTGGTACATTCTGCACCCGCTGGAATTGCTGCGCTTTCCGTATATCGTTTCTGTTATGAACGACGCTCCGGCAGTg < 2:362425/140‑1 (MQ=255) ttCTGTCGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGACGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAACCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCAt < 1:660324/143‑1 (MQ=255) tCTGACGATTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGAGAACGCTGGAACATGCTGCACCCGCTGGCAACTCCGCGCGTTCCGCATAGCGCAAAGCGGATGAACGACGCTCCGGCGCTGGCATc > 2:610135/1‑143 (MQ=255) gTTCATAGCGTCACCTCCTTCACCGACCGGGCGCGTGATGGTCAGGATCTGGAACGCTCCAACATGCTGCACCCGCTGGAAACTCCGCGCGTGCCGTATATCGCTTAGCTGCTCAACTGCGCTCCGGCAGTGGCATCTACCGa < 2:92905/143‑1 (MQ=255) taGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACGCCGCGCGGTCCGTATATTGCTTAGGTGATGGATGACGCTCCGGCAGTGGCAACTccccgccata > 2:618983/1‑136 (MQ=255) cGTGACTTCCTTCACTGAGCTTGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGa < 2:320605/143‑1 (MQ=255) cGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCTGGCAGTGGCTTCTAATGACTatatta > 1:254472/1‑141 (MQ=255) cGTGACCACCTACACCGAGCTGCAGCACGAAATTGAGTATTGTGATCGCTGGAACATGCTGCACCCGCGGTAACCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGa < 1:137700/143‑1 (MQ=255) tcctTTCCCGAGCGTGCGCGTGATGTCCATGATTGTGAACGCTGGAACATTCTGCATCCACTGGAAACTCCGCGTGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACtttt < 2:376153/143‑1 (MQ=255) cctCACCGACCTGGCGCGTGACGTTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACCCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCg < 1:47756/141‑1 (MQ=255) ttCGCCGAGTTGGGGGGTCATGGTCAGGATTGTGAACCCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATTTCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGc < 2:313520/143‑1 (MQ=255) ggCTGGCGGGGTATGGTCAGGATTCGTAACGCTGGAACATGCTGCACCCGCGGGAGACTCCGCGCGTTGCGGATATCGCTTAGGCGATGCACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAg < 2:111595/142‑1 (MQ=255) gcgcGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGATCCg > 2:310842/1‑143 (MQ=255) cgcgTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGt > 1:665111/1‑143 (MQ=255) cggtggtgGTTAGGATGGTTAACGTTGGATCGTGTTTCAGCCGCTGGAATCTCGGCGCGTTCGGTATATCGCTTAGGGGATGAACGACGCTTCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTa < 2:28709/141‑1 (MQ=255) taTGGTCGTGATTTTGCGCGCTGGAACATGCTGCACCCGCTGGAACCTCCGCGCGTTCCGTATATCGCCTAGCTGGTGAACGACGCTCCGGCAGTGGCATCTACCGACTTTATGATACTGTTCGCTGAGCAGGTCCGTACTTa < 2:450602/142‑1 (MQ=255) gATGGTCAGTATTGTGAACGCTGGAACATGCTGCACTCGCTTGAATCTCCTCGCGTTTCTTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTa < 1:2875/143‑1 (MQ=255) gacAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCATAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGGAACTGTTCGCTGAGCAGGTCCGGACTTACGTa > 2:527617/3‑143 (MQ=255) gTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCCTCTACCGACTGTATGAACCTGTCCTCTGGGCACGACAGTAATTACTTa > 1:616154/1‑143 (MQ=255) tCAGGATTGTGAACGCTGGAACAAACTGCACCCGCTGGAAACTCCGCGAGTTACGTATATCGCTTAGATGATGAACGACGCTACAGCAGTGGCATCTACAGAATATATGAAACTGTACGATCAGAAGGTACGTAAGTACGTaa > 2:722248/1‑142 (MQ=255) ggATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCgg < 1:446902/143‑1 (MQ=255) gATTGTGAACGCTGGAACATGCTGAACCCGCTGGAAACTCCGCGCGATCCGTGTATGGCTTAGGTGATGAACGACGCACCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGACCAGGTCCGTACTCACGTACCGGc > 2:77880/1‑143 (MQ=255) gtgAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGCAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTgac > 1:321385/1‑143 (MQ=255) aaCGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGCCTATATGAAACTGTTCGCTGAGCAGTTCCGTACTTACTTACTGGCTtacgac > 1:142293/1‑143 (MQ=255) aaCGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCTTTCTTACGTACTGGCTgacgaa > 1:718506/1‑142 (MQ=255) gcctgAACATGCTGCTTCCGCTGGAATCTGCGCGCGTTCCGTATATCGCTTAGGTGATGATCGACGCTCCTGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTTCGTACTTACGTACCGGCTGACGACTAc < 2:628914/139‑1 (MQ=255) gCTGGAACATCCTGCTCCCGCTGGAAACTCCGCGCGTTCCGTATATCGTTTAGGTGATGAACTACGCTCCGTCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAc < 1:142763/143‑1 (MQ=255) aTGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGCTGAACGACCCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTAAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACt > 1:457797/1‑143 (MQ=255) tgctgcACCCGCGGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGACAACTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTGACGTACCGGCTGACGACTACCGCGTACTg > 2:669720/1‑143 (MQ=255) ccGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGa > 1:576209/1‑143 (MQ=255) aaaCTCCGCGCTTTCTGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACGGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTATCGCGTACTGGGTACTGATGGCTTc < 2:126609/143‑1 (MQ=255) aaCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCAGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCg < 1:499500/143‑1 (MQ=255) cTCCGCGCGTTCCGTATATCGCTTATGTGATGATCGACGCTCCGGCAGTGGCATTTACTGACTATATTAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGt < 1:374979/143‑1 (MQ=255) tCCTCGCGTTCCGTATATCGCTTAGGTGATGAATGTCGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTc < 1:375540/143‑1 (MQ=255) gTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCATTCCGa > 1:376471/1‑143 (MQ=255) ttCCGTATATCGCTTAGGTGCTGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCTTTCCGAc > 2:402865/1‑143 (MQ=255) ttCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGAc > 1:33067/1‑143 (MQ=255) gtatCGCTGAGGTGATGACCGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGt < 2:454235/142‑1 (MQ=255) | GGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGT > NC_000913/125254‑125521 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |