| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,321,822 | T→C | 12.3% | F594L (TTC→CTC) | atoS → | sensory histidine kinase AtoS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,321,822 | 0 | T | C | 12.3% | 76.6 / 4.5 | 45 | F594L (TTC→CTC) | atoS | sensory histidine kinase AtoS |
| Reads supporting (aligned to +/- strand): ref base T (18/21); new base C (3/3); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01 | |||||||||||
CGCTGCAAAAAAAGATCTTCGATCCCTTTTTCACCACCAAAGCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGAAACACATTGT > NC_000913/2321682‑2321964 | cGCTGCAAAAAAAGATCTTCGATCCCTTTTTCACCACCAAAGCCTCAGGAACCGGGATTGGGCTGGGGTTAAGTCAACGCATCATTAATGCGCATCGGGGTGATGTTCGCGTCGCAAGTTTGCGGGGCGACGGCGCAGCCtgt > 1:219840/1‑141 (MQ=255) gCAAAAAAAGATCTTCGATCCCTTTTTCACCACCAAAGCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGCTTGGCGGGCTACGGCGGAGCCTTCACGc > 2:236265/1‑143 (MQ=255) ttCGATCCCTTTTTCACCACCAAAGCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGAt > 2:36764/1‑143 (MQ=255) gtggATCCCTCTGTCAGCACCAAAGGCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGAt < 1:504528/140‑1 (MQ=255) gtCGATCCCTTTTTCACCCCCAAGTCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCTCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGAt < 2:396076/142‑1 (MQ=255) aTCCCTTTTTCACCACCAAAGCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCCTCACGCTTATTTTACCGATCAAc > 1:666499/1‑143 (MQ=255) aTCCCTTTTTCACCACCAAAGCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCCTCACGCTTATTTTACCGATCAAc > 1:666497/1‑143 (MQ=255) aTCCCTTTTGCACCACCAAAGCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAAc < 2:246430/143‑1 (MQ=255) ttttCACCACCAAAGCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCCTTAATGCCCATCAGGGTGATATTTGCGTCGCCCCTCTCCCGTGCCACGGCGCAACCTTCACGCTTATTTTTCCGCTCAACCCGcgg > 1:598878/1‑141 (MQ=255) tcttCACCCACAATTCCTCAGGATCCGGGCTTGGTCTGGCGTTAAGTCCACGCATGATTAATGCCGATCTGGGTGATATTCGCTTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAg < 2:74175/141‑1 (MQ=255) tCACCTCCATGGCCGCCGTAATCGGGCTTTGTCTGGCGTTCAGTGAACGCATGATTAAGGCCGATCAGGGTGTTATTCGCGTCGCCAGTTTGCCGGTCTACGGCGCAACCTTCACTCTTATTTTACCGATCAACCCGCAGGGa < 2:262709/143‑1 (MQ=255) tCACCACCAAAGCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCCTCACGCTTATTTTACCGATCAACCCGCAGGGa < 2:666499/143‑1 (MQ=255) tCACCACCAAAGCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCCTCACGCTTATTTTACCGATCAACCCGCAGGGa < 2:666497/143‑1 (MQ=255) ccaAAGCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGCAATCAg > 2:176485/1‑143 (MQ=255) aGGAACCGGGCTTGGTCTGGCATTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTAGCGTCGCCAATTTGCCGGGCTACGGCGCAACCTTCACGCCTCTGTTACCGATCAACCCGCAACGAAATCAGACCGTACGAc > 2:663857/1‑143 (MQ=255) aaCCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCGGCAGGGAAATCGGCCTGGATGCCTGc > 1:389290/1‑143 (MQ=255) cGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATTTTACTGCTGCCAGGAGGCCGGGCTACGGCGTAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTAt > 1:141150/1‑143 (MQ=255) cGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACATTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTAt > 2:364784/1‑143 (MQ=255) cGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTAAGGCGCAACCTTCACGCTTACGTTACCGATCAACCCGCAGGGAAATCAGACGGTATGACTGCTAt > 2:619846/1‑143 (MQ=255) cGGGCTTGGTCTGGCATTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTGACCAGTTTGCCGCGCTACGGCGCAACCTTCACGCATATGTTACCGATCAACCCGAAGAGAAAGCAGACTGTAAGCCTGcaaa > 2:222159/1‑140 (MQ=255) ggCTTGGTCTGGCGTTAAGTCAACGCATCAGTAATGCCCAGCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTa < 1:734483/143‑1 (MQ=255) ggCTTGGTCTGCCGTTAAGTCAACGCCTCATTAATGCCCATCAGGGTGATGTTAGGTACGCCATTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTa < 1:172154/143‑1 (MQ=255) cTTGGGCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGTGCTACGGCGGAATCTTTTTGCTTATTTTGCCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAAt < 2:125901/143‑1 (MQ=255) ttGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGAATGTATGACTGCTATTAATc > 1:418065/1‑143 (MQ=255) tCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGACGGGCTACGGCGCAACCCTCACGCATATTTTACCGATCAACCCACAGGGAAATCAGACTGTATGACTGCCATTAATCACATACTAATAGTGga > 2:443321/1‑143 (MQ=255) aCGCATCATTAAGGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGgatga < 1:551881/143‑1 (MQ=255) tcatTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCAAGCATAGGGTTCCGATCAACCCGCAGGGAAATCAGACTGTCTGCCTGCTATTACTTGCTTCCTTATTGTTGATGATGAAg > 1:480425/1‑143 (MQ=255) atTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTATCCAGGCTACACCGCAACCTTCACGCTTATGTTACCGATCAACCCGCAGAGAAATCAGACTGTATGACTCCTATTAACCGCAACCTTATCGTGCGCGATGAAgct > 2:506142/1‑141 (MQ=255) gggcATCAGGGGCATTCTAGCGTCGGCAGTTTTCCGGGGTACGGCGGAACTTTCACGCTTATTTTACCGATCAACCCGCAAGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGtt < 2:768747/140‑1 (MQ=255) tCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTACTGTGGATCATGAAGATAATGTTAGCCg > 2:656977/1‑143 (MQ=255) atatTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGa < 1:36764/143‑1 (MQ=255) tCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACCGCTATTAATCGCATCCTTCTTGTGGAAGCTGAAGATAATGTTCGCCGTAAGCTcaccac > 2:21094/1‑143 (MQ=255) gcgTCGCTAGTTTGCCGAGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCg < 1:614279/143‑1 (MQ=255) cgTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATACTGTTCGCCGTATGCTGAGCACCGc > 1:750614/1‑143 (MQ=255) gTCGCCAGTTTGCCGTGCTACGGCGTAACCTTCTCGTTTATTTTACCTATCATTCCGCAGGGTAATCAGATTGTTTGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTGCGCCGTATGCTGAGCACCGCt < 1:397873/143‑1 (MQ=255) gCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATCGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCtttt > 2:47570/1‑143 (MQ=255) aGTTTGCCGGGCTACGGCGCTACCTTCACGATTATTTTACCGATCAGCCCGCAGAGAAAGAAGACTGTAGGACGGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCa < 1:189844/143‑1 (MQ=255) gcatGCCGGGCAACGGCGCCATCCGCACGCTTATTGTACCGATCAACCCGCAGGGAAATCAGACTGTATTACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCAc < 1:236265/140‑1 (MQ=255) gTTTGCCGGGCTACGTCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCAc < 2:306382/143‑1 (MQ=255) ttggcggggCTAGGGCGCAACCTTCTCGCTTATTTTACCGGTCAAGCCGCAGGGAAATCAGACTGTATGACTGCTAGTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACt < 2:157363/137‑1 (MQ=255) ccGGGCTACGGCGCATCCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACaa < 1:364784/143‑1 (MQ=255) gggCTACGGCGTAACCTTCACGTTTATTTTAATGATCAACACGAAGGGAAATAAGACTGTATGACTGCTATGAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACAAgg < 1:271694/143‑1 (MQ=255) ggCTACGGCGCAACCTTCACGCTTATTTTACCGATCATCCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGa < 1:579153/143‑1 (MQ=255) tACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAACACCGCTTTTGCACTACAAGGACTc > 2:272687/1‑143 (MQ=255) tccacAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGaa < 1:96179/139‑1 (MQ=255) gcgcAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGaaa < 2:480425/143‑1 (MQ=255) ttCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAACGTTCGCCGGATGCTGAGCACCGCTTTCGCACTCCAAGGGTTCGacactcctgct > 1:432741/1‑136 (MQ=255) ttCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTAATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCCTTTGCACTACAAGGATTCGAAACACATtgt > 2:9102/1‑143 (MQ=255) | CGCTGCAAAAAAAGATCTTCGATCCCTTTTTCACCACCAAAGCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGTGATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATCAACCCGCAGGGAAATCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGAAACACATTGT > NC_000913/2321682‑2321964 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |