| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,399,516 | C→A | 16.9% | R217L (CGT→CTT) | nuoG ← | NADH:quinone oxidoreductase subunit G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,399,516 | 0 | C | A | 16.9% | 67.9 / 3.6 | 33 | R217L (CGT→CTT) | nuoG | NADH:quinone oxidoreductase subunit G |
| Reads supporting (aligned to +/- strand): ref base C (11/16); new base A (2/4); total (13/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GAGGAAGTAGTGGTTTACCGTACCGTTGTAACGGTTTTCGATACGACGCAGTTCGCCGTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCA > NC_000913/2399380‑2399657 | gagGGAGTGGTGGTTTACCGTACCCTTGTAGCGGTTTGCGATACGACGCAGTTCGCCGTAACGTTCACTGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTAAGGTTGt < 2:361318/143‑1 (MQ=255)gagGAAGTAGTGGTTTACCGTACCGTTGTAACGGTTTTCGATACGACGCAGTTCGCCGTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGt > 2:144285/1‑143 (MQ=255) agGAAGTAGGTGGTTACCGTACCGTTGTGGCGGTTTTCGATACGGCTCAGTTCGTCGTAACGTTCACCGGGGCGGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTa < 2:707456/143‑1 (MQ=255) agtagtGGTTTACCGTACCGTTGTAACGGTTTTCGATACGACGCAGTTCGCCGTAACGTTCACCGGGGCTGATGCTACAGCCGATGGAACATTGCTGGCAGATGCTAGGAGAAAACTGAATATCCCATTTGCGGTTGAAACGc > 2:112550/1‑143 (MQ=255) ccgtaccgtTGTAACTGTTTTCGATACGACGCAGTTCGCCGTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGtt < 1:21915/143‑1 (MQ=255) ccgtaccgtTGTAACCGTTTTCGAGACGACGCAGTTCGCCGTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGtt < 1:442473/143‑1 (MQ=255) cgtaccgtTGTAACGGTTTTCGATACGACGCAGTTCGCCGTAACGTTCACCGGGGCTGATGTTACAGCCGGTGGGACATTGCTGGCAGATGCTCGGCGCAAACTGCTTATCCCATCTACGTTTGTAACGATCGAAGTGAGtta > 1:36279/1‑142 (MQ=255) acggtTGTATCGGTGTTCGATAGCACGCGGTGCGCCGTATCGTTCCCCGGGGCTGCTGTTACAGTCGATGGGACATTGCTGGCAGATGCTCGGCGCCAAGTGCATAGCCCATTTACGGTGGTAACGCTCGGAGGTCGTATTGt < 2:272824/140‑1 (MQ=255) accttTGTAACGGTTTTCGATACGACGCAGTTCGCCGTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGt < 1:449455/143‑1 (MQ=255) ttttCGATACGACGCAGTTCGCCGTAACGTTCACCTGGGCTGATGTTACAGCCGATGGAACATCGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTCTTGTCGGTCAAtcagcc > 2:207591/1‑138 (MQ=255) ttttCGATACGACGCAGTTCGCCGTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCCAACTGCATATCCAATTTAAGGTTGTAACGCACGGAGTGCGATTTGTCGGTAAATACGcc > 1:568147/1‑143 (MQ=255) ttttCGATACGACGCAATTCGCCGTAAAGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTACTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGAGCGTTTTGTCAGTAAATaagtc > 2:300678/1‑139 (MQ=255) tACGACGCAGTTCGCCGTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCggg > 2:395461/1‑143 (MQ=255) acgCAGTTCGCCGTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGAAGGTCAGGCaaa > 1:260436/1‑143 (MQ=255) cGTAACGTTCACCGGGACTGATGTTACAGCCGATGGAACATTGCTGGCAGATCCTCGGCGCAAACGGCATATCCCACTTAAGGTTGTAACGCACGGAGTACGATTTATCGGTAAATAAGCCAGGCGGGACAAATTAGACCAgg > 2:487152/1‑143 (MQ=255) gtcggATGGTACAGCTGTTGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTTCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCg < 2:401455/139‑1 (MQ=255) gtcTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTAAGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCg < 1:289749/141‑1 (MQ=255) cTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAACCTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCAGTAGGGCAAATTTCGACCAGGTTACAGGAAAATTCGCt > 1:711396/1‑143 (MQ=255) cTGATGTGACAGCCGCTGGAACATTGCTGGCAGATGCTCGGCGCAGACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCt < 2:310593/143‑1 (MQ=255) tgttGTTACAGCCGATGTCACATTGCTGGCAGATTCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCtt < 1:123956/140‑1 (MQ=255) aTGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCCCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTc > 2:262586/1‑143 (MQ=255) tACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGc < 1:442706/143‑1 (MQ=255) tgAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTAAGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACAAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCtt < 1:526167/142‑1 (MQ=255) cATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCgg < 1:717599/143‑1 (MQ=255) cAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGaa < 1:678190/143‑1 (MQ=255) cGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCACGCGACCGAAGTAGACGt > 2:583705/1‑143 (MQ=255) ggCGCTATCGGCTTACCCCGTTTGAGGTGGTGACGCTCGGAGTTCGTTTTTTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACGGGACAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGtt < 2:143842/143‑1 (MQ=255) ggCGCAAACTGCATATCCCCTTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGtt < 1:332523/143‑1 (MQ=255) gcgcAAACTGTATATCCCATTTGCGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTg < 1:578863/143‑1 (MQ=255) aaCTTCATATCCCATTTACGGTTGTAACGCTCGGATTGCGTTTTGTCGGTAAATACGCCGGTCTGGCAAATTTCGACTAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTg < 1:678052/143‑1 (MQ=255) aaCTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTg < 1:696679/143‑1 (MQ=255) tCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCAGTAAATACGCCGGGCGGGCAAATTTCGACCAGGGGACCGGAAGATTATCGTTCCAGCGTGCCGTGTCCCGGGCGACCGACGCAGACGTCGTCGCGCGTACGGTa > 2:309193/1‑143 (MQ=255) aCGGTTGTAACGTGCAGAGTGCGTGTTGTCGGTAAATACGGCGGTCGGGCAAATTTCGACCAGGTTACCGGCACATCCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCa < 1:616689/143‑1 (MQ=255) | GAGGAAGTAGTGGTTTACCGTACCGTTGTAACGGTTTTCGATACGACGCAGTTCGCCGTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCA > NC_000913/2399380‑2399657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |