| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,661,969 | C→G | 100% | V55L (GTT→CTT) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,969 | 0 | C | G | 99.0% | 55.8 / ‑6.8 | 24 | V55L (GTT→CTT) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (12/11); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCA > NC_000913/2661840‑2662105 | aGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAGGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTa < 2:517270/143‑1 (MQ=255) gcgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACg < 2:108995/142‑1 (MQ=255) gccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACCGATAAACACCGACTGGTCCACGCACGCTAGAAAGCAGACCAttcttcagca > 2:616294/1‑138 (MQ=255) tGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGaaaa < 2:451495/143‑1 (MQ=255) gggTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACCGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACagtagt > 2:554179/1‑143 (MQ=255) aTCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGACACCAGACCACTTTTACTCAGACGGGAAAACAATTACTCCAGAt > 1:664972/1‑143 (MQ=255) aTCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGAt > 1:15659/1‑143 (MQ=255) aTCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTGGTTCCAGAt > 2:724489/1‑143 (MQ=255) tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGGCTGGTCTGCGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTGCCAGATAAGAAAGGGAAAt > 2:577773/1‑143 (MQ=255) cGGCGCGAATGACTTCGCCAGCGGCGAGGCCGCTGGCATGTTTGCCTAACAGATAACCAGCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTccc < 2:221732/143‑1 (MQ=255) ttCGCAAACGGCGATGCCGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa < 1:473188/143‑1 (MQ=255) aaCGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGTCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAg < 1:45480/143‑1 (MQ=255) taTGCTGCTGGCATCTTTGGCTAACAGATAACCGCCGGCTGGTCCACGTACGCTGGAAAGCAGACCATTTGTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAATTATCAGCCAACg < 2:677343/142‑1 (MQ=255) tgctgTCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTCTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGAGAAATTCCCTGACGTTCAGAAATATCAGCCAACGGTaac > 2:126345/1‑141 (MQ=255) gttcgCATCTTTGCCTACTAGATAAGCACCGCCTGTTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGCTGGGAAACCACTTGTTCCAGATAAGAAAGGGACATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCg < 2:281163/139‑1 (MQ=255) tgGGATCGTTGGCGAACAGATAACCTCCGGCTGGTCCAGGTACGCTGGAAAGCGGACCATGTTTGCGGAGACGGGAAAACAGTTGTTTCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCggg < 2:88904/143‑1 (MQ=255) tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGCAAGCAGACCATTTTTACGCAGACGGGCAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTACGGAAATATCAGCCAACGGTACCGGGCCCGCtt > 1:651380/1‑143 (MQ=255) gCCTAACAGATAACCGCCGTCGGGTCCACTTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACATGTGTTCCAGATATGAAAGGTAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCag < 2:375681/143‑1 (MQ=255) tAACAGATAAGCACCGCCTTATCCACGTACGCTGGAAAGCAGACCATTCTTACGCAGACGGAAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGACCGCTTCAGAGt > 2:420676/1‑143 (MQ=255) tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTATCGGGCCAGCTTCAGAGt > 1:23019/1‑143 (MQ=255) tAACAGATAACCACCGCCTGGACCACGTACGCCAAAAAGCAGACCAATCATACGCAGACGGAAAAACACGTGCTCCAGATAAGAAAGGGAAATTCCCTGCAGTTCGGAAACATCAGCCAACGGCACAGACCCCGCCTCagcga > 2:641963/1‑140 (MQ=255) aGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAACGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTTCCGCGCCCGCTTCAGAGTTGAg > 1:154530/1‑143 (MQ=255) ccATGTACGCTGGAAAGCTGATCATTTTTACGCAGATGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGc < 1:577773/143‑1 (MQ=255) tGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGCAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGACa > 1:665761/1‑143 (MQ=255) | AGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCA > NC_000913/2661840‑2662105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |