Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,041,632 T→A 100% V107E (GTG→GAG)  ygfZ → folate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,041,6320TA100.0% 100.6 / ‑6.7 47V107E (GTG→GAG) ygfZfolate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (20/24);  minor base C (1/1);  total (21/26)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATG  >  NC_000913/3041492‑3041770
                                                                                                                                            |                                                                                                                                          
cgccCATTGTGACGCATAATGTAAAATGTGGAGCAATTTACGTCTGTCCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAgaga                                                                                                                                          <  1:2905/143‑1 (MQ=255)
cgccCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAgaga                                                                                                                                          <  2:100382/143‑1 (MQ=255)
 gccCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGAc                                                                                                                                         >  1:179765/1‑143 (MQ=255)
        gCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAgaga                                                                                                                                          <  1:162630/135‑1 (MQ=255)
         cGACGCCAAAGGCAAAATGTGGAGCAATTTACGTCTGTGCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCgcgc                                                                                                                                 <  2:350447/143‑1 (MQ=255)
          gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGcc                                                                                                                                >  2:669118/1‑143 (MQ=255)
          gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGcc                                                                                                                                >  1:751358/1‑143 (MQ=255)
              ccAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGACGCAGCGTGCGTGAACCGCAGCTGACAGATCTGAAAAAACAAGCGAAATTATCGATAGAGACCATCGCGCCAgac                                                                                                                            >  1:762520/1‑143 (MQ=255)
                aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGAATGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACTATCGCGGCagaaga                                                                                                                          >  1:66118/1‑143 (MQ=255)
                 aaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTCAAGAGACAATCGCGCCCgacgac                                                                                                                         >  2:181676/1‑143 (MQ=255)
                 aaGGGAAAATGTGGAGCAGTTTCCGTCGGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacgac                                                                                                                         <  2:722923/143‑1 (MQ=255)
                     tAAACTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGAGGTCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGc                                                                                                                     <  2:697432/143‑1 (MQ=255)
                         aggtgGAGCAATGTACGTCTGTTGCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtgt                                                                                                                 <  2:737942/141‑1 (MQ=255)
                                          tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGTACGGCGCAGTGTGTGTGAATCGCAGCTGACTGAACTGAAAAAATATGCGGTGTTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                                <  1:505878/143‑1 (MQ=255)
                                                          gATCGCTCTGCATGGATTGCACCGCGCCGCGTGCGTGAACCGCAGCGGACTGAACTGAAAAAATATGCGGTCTTCCCTACAGAGGCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc                                                                                <  1:162272/143‑1 (MQ=255)
                                                           aTGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGCCTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACCAGCGTGTGCTGCTTGTTGTTGCCGGTTTTCAGgctcgcgca                                                                               >  1:156654/1‑142 (MQ=255)
                                                            tGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCCGACGACGAGCGTGTGCTTCTTGGGGATCCCCGTTTTCCGGCGcgcgccc                                                                              >  2:44380/1‑142 (MQ=255)
                                                              gCTTTGCATGGATTGGACGTCGCAGCGTTCGTGAACCGCAGCTTACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCTTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcg                                                                            <  2:490016/143‑1 (MQ=255)
                                                                tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTATCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGCGTTGCCGGCGTTAAGGCCcgcgcggcgcc                                                                          >  2:654970/1‑142 (MQ=255)
                                                                 tttcATGGATTTAACGGTGCAGCGTGCGTGAACCGAAGCTGACGGAACTGAAAAAATATGCGGTATTCACAAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg                                                                         <  1:136383/140‑1 (MQ=255)
                                                                 ttGCATGGATTGAATGGCGCGGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg                                                                         <  1:228140/143‑1 (MQ=255)
                                                                  tGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGATTCCATCGCGACAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg                                                                        <  1:437928/143‑1 (MQ=255)
                                                                   gCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCCGGCCCGCGCCGCCCCGGc                                                                       >  1:758186/1‑143 (MQ=255)
                                                                   gCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGc                                                                       >  1:511811/1‑143 (MQ=255)
                                                                      tGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTCCCGGCGTTCAGGCGCGCGCCGCGCTGGCaca                                                                    >  2:588593/1‑141 (MQ=255)
                                                                       ggATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt                                                                   <  2:654981/143‑1 (MQ=255)
                                                                          ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGAATGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtctc                                                                <  1:338062/143‑1 (MQ=255)
                                                                           tGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTCCCGGTCTTCAGGCGCGCGCCGCGCTGGCAAAtctat                                                               >  2:640307/1‑141 (MQ=255)
                                                                           tGAACGGCGCAGCGTGCGTGAACAGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTATAAAGAGACCATCGCGCCAGAGGACGAGCGTGTGCTGCTTGGTGTCGACGGGATTCAGGCGCGCGCCGCGCTGGAAAAGctct                                                               >  2:22326/1‑143 (MQ=255)
                                                                            gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtt                                                              <  1:447689/143‑1 (MQ=255)
                                                                            aaaCGGCGCATCGCGCGTGAACCGCGCCTGACTGAGCTGAAAAAAAATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCGTGGTGTTTCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCGCtt                                                              <  2:554046/142‑1 (MQ=255)
                                                                               cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGCGACCATCGCGCCAGACCACGAGCGTGTGCTGCTTGGTGTTGCCGCTTTTCAGGCACGCGACGCGCTGACAAAactcgtaaa                                                           >  1:288017/1‑138 (MQ=255)
                                                                               cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGTCGCGCTGGCAAATCTCTTTAg                                                           >  1:286017/1‑143 (MQ=255)
                                                                                   gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTACCGATTTTAAGGCGCGCGCAGCGCTGGCAAATCTCTTTCGCGaa                                                       >  1:379531/1‑143 (MQ=255)
                                                                                   gcACCGTGCGTGAACCGCAGCTGCCTGCACCGTAACAATATGCGCTATTCTCTATCGGGCCCCTCGCGCGACACCACGAGCGTGTGCTGCTTGGTGTTGCCGCTGTACAGGCGTGCGCCGCGCTGGCAAATCTCTTTAGCGaa                                                       <  2:582110/143‑1 (MQ=255)
                                                                                      gcgtgcgtGAACCGCAGCTGACTGAACTGAAAAAATATGCTGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTg                                                    <  1:738916/143‑1 (MQ=255)
                                                                                                 gcGCAGGTGACTGACCTGAGAAAAGATGCGGTAGGCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaaga                                         <  1:420785/142‑1 (MQ=255)
                                                                                                     aGCGGACTGAACTGAAAAAATAGGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGaaaaa                                     <  2:176173/143‑1 (MQ=255)
                                                                                                       ctgactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCACATCTCTTTAGCGAACTGCCCTCGAACGAGATACa                                   >  1:123757/1‑143 (MQ=255)
                                                                                                         gactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAgg                                 <  1:669118/143‑1 (MQ=255)
                                                                                                           ctgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa                               <  2:511811/143‑1 (MQ=255)
                                                                                                            tgaactgaaAAAATATGGGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAg                              <  2:5272/143‑1 (MQ=255)
                                                                                                                 gtgaaaaaTAGGCTGGAGTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTAGATGGTTGCCAAGCAATAGCCGCGATGGCAAATCTCTTTAGCGAACTGCATTAAAAAAAAAAACAGGTAGTCaaa                         <  2:461076/140‑1 (MQ=255)
                                                                                                                    aaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa                      <  1:443950/143‑1 (MQ=255)
                                                                                                                    aaaaaTATGCGGAAGGCTAGAAAGACACCACCGCGCCAGACGACGAGCGTATGCTGCTTGGTGTTGCCGCTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa                      <  1:188064/143‑1 (MQ=255)
                                                                                                                      ttatatTCGGTATTCTCTTACGAGTCCACTGCGCCGGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCCCGCGCCGCGCTGGCAAATCTTTTTAGCGAACTGCCTTCGAATGAAAAACAGGTAGTCAAAGAAgg                    <  2:228533/141‑1 (MQ=255)
                                                                                                                       gagatGCGGTAGTCTCTGAAGCGTCCATCGTGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCTGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgc                   <  2:640688/140‑1 (MQ=255)
                                                                                                                               gTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAG‑AAAACAGGTAGTCAAAGAAGGCGCGACCACt          >  1:456993/1‑143 (MQ=255)
                                                                                                                                      ctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCACATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGAAGTCAAAGAAGGCGCGACCACTTTGCTa    >  2:739860/1‑143 (MQ=255)
                                                                                                                                        aaaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTAAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCAAAATACATGAGAAAAAAAAACAGGTAGACAAAGAAGGAGCAACCAATTTCCTATg  >  1:651964/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                          
CGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATG  >  NC_000913/3041492‑3041770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑