| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,184,543 | C→A | 100% | P1100Q (CCG→CAG) | rpoB → | RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,184,543 | 0 | C | A | 100.0% | 119.0 / NA | 42 | P1100Q (CCG→CAG) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (26/16); total (26/16) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA > NC_000913/4184403‑4184681 | acTGGCAGTTAAACGCCGGACCCAGCCAGGTGACAAGCTGGCAGGGCGTCACGGTAACAAAGGTCTAATTTCTAAGCGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCCTACTGAACCAGc < 1:673455/142‑1 (MQ=255) cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCAGTAGACATCGTACTGAACCAGCt > 1:677123/1‑143 (MQ=255) gCGGTTAAAGCCCGTATCCAGCTTGTTGACACTCTGGCAGGTCGTCACGGTAACAAGGGTTTAATTTCTAATATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTggg < 2:591473/143‑1 (MQ=255) aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAcc > 2:133253/1‑143 (MQ=255) aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCg > 2:555511/1‑143 (MQ=255) cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGATGAAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGAAGGTAGCCATCGTACAGAACCAACTGGGCGGCCCGta > 2:80468/1‑142 (MQ=255) cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa > 1:536048/1‑143 (MQ=255) tGACAAGATGGCAGGTCGTCACGGTAACAAGGGCGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTCGACAACGTACTGAACCAGCTGGGCGTACCGTCGCGTCTGAACATCGCTc > 1:293713/1‑143 (MQ=255) gACAACATGGCCGGTCGTCACGGTTACAAGCGTGTAATTTATAAGATCAACCCGATAGAACATATGTCTCACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCa < 2:413377/143‑1 (MQ=255) aGATGTCAGGTCGTCACGGTAACAATGGTGTAATTTCGAAGATCGACCCGATCGAAGATATGCCTTACGATGAAATCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATc < 2:654715/143‑1 (MQ=255) gATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGACGTACCGTCTCGTATGAACATCGGTCAGATcc > 1:634874/1‑143 (MQ=255) aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCATATGAACACCGGTCAGATACTCGaaa > 1:177427/1‑143 (MQ=255) gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGCTCAGATCCGCGAAAcc > 2:451695/1‑143 (MQ=255) cggcACGGGAACGAGGGGGTAATTTCTAAGAACAACCCGATCGAAGATATGCCTTACGATGAAACCGGTACGCCGGTAGCCATCGTACTGAACCAGCTGGGCGCACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCa < 1:30076/140‑1 (MQ=255) aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGACAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTg > 1:307878/1‑143 (MQ=255) gTAGCAAGGGTGTAATTTCGGAGATCAACCCGATCGAAGGTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAGCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGt < 2:339722/143‑1 (MQ=255) tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGaaa < 1:548136/143‑1 (MQ=255) tCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCGGGGGGTGG‑CTGCGAAAGGTAt > 2:85058/1‑143 (MQ=255) tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATGGGGCACATCCTCGAAACCGCCCCGGGTATGG‑CTGCGAAACGTACCg > 2:436146/1‑143 (MQ=255) aaGATCAACCCGATCGAAGATATACCTTACAATAAAAACATTACGCTGGTAGACATCGTACTGAACCAACTACGCTGACGGTCTCGTATGGACATCGCTCAGACCCTCGAAACCCACCTGGATATGT‑CTGCGAAAGGTCTCgc > 2:506119/1‑142 (MQ=255) aTCAACCCGATCGAAGATATACCCTATGATACTGGGTGTGGGCCGTGAGACATCGGACGGAGCCAGCGGGGCGTACCGGCTCGGATGAACAGCGGGCAGATCATCGAAACACACCTGGGAATGG‑CGGCGAAAGGTAGCGGCGa > 2:340627/1‑143 (MQ=255) aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAg > 1:136056/1‑143 (MQ=255) gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACAGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAACCTCGAAACACACCTGGGTATGG‑CTGCGAACGATAACGGCGACAAGaacaacca > 2:189227/1‑141 (MQ=255) ccTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGCCAAGATCAACGCCATGCTgca > 1:537994/1‑141 (MQ=255) tctattaaaaCGGTACGCCGGTAAACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag < 1:366440/137‑1 (MQ=255) tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATCCTGAAAcacaagc > 2:522922/1‑138 (MQ=255) gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGCAACAgcagca > 1:94408/1‑143 (MQ=255) gcGGGACGCCGGTAGACATCGTACTGAAGCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa < 2:119916/142‑1 (MQ=255) ggTGCGCCGGTAGACATCGCACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGt < 1:134138/143‑1 (MQ=255) tACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg < 1:629093/143‑1 (MQ=255) gCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCACCGCCATGCTGAAACAGCCGCCAGAAATccaaa > 1:529095/1‑139 (MQ=255) ccaggaGAAATTGTACTGAACCAGCTGGGCATACCGTAGCGTATGAACAGCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaa < 1:164393/138‑1 (MQ=255) ccGGTAGACATCGTACTGAACCAGCTGGGCGTCACGTCCCGCATTAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaa < 1:727171/143‑1 (MQ=255) cGGTAGACATCGTACTGAACCAGCTGGGCGGACCGTCTCGTCTGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaaa < 1:555511/143‑1 (MQ=255) gggaGACCTCGTACTGAACCAGCTGGGCGTTCCGGCTCGTATGAACGTCGGTCAGATCCGCGAAACCCACCTTGAGATGG‑CTGCGAAAGGTATCGGCGACCAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc < 2:107451/140‑1 (MQ=255) gTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTCCGAAAGGTATCGCCGACAAAATCAACGCCATGCTGAAACAGCAGCAAGAAGCTGCGAAACTGCGCGCAtt > 2:616876/1‑143 (MQ=255) cggAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCATATCCTCGAAACCCACCTGGGTGTGGTGTGTGAAACG‑AGCAGAGCAAAATTCGACATGATGGTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAt < 1:769222/141‑1 (MQ=255) cTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAt > 1:469381/1‑143 (MQ=255) tGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc > 1:96839/1‑143 (MQ=255) gAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGGCGCGAAACAGCGCAAAGTCAtac > 2:74560/1‑141 (MQ=255) aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACACGATCAACGCCATGCTGCAACAGCAGCAAGAAGTCGCCAAACTGCACGAATTCATCCa > 1:361510/1‑143 (MQ=255) aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCa > 1:94341/1‑143 (MQ=255) | TCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGG‑CTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA > NC_000913/4184403‑4184681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |