| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | 100% | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 100.0% | 91.4 / ‑6.7 | 35 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (19/15); minor base A (0/1); total (19/16) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCT > NC_000913/759117‑759387 | aCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACCTTACCACCACCGAAGAAAAAATCTGGCTCCa > 2:322739/1‑143 (MQ=255) cTTCCACGTCGGTTCATTTGCCAGCGGCCAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAAc < 2:478213/143‑1 (MQ=255) ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 1:196649/143‑1 (MQ=255) ttCAACGTCGGGTCATTTGCCAGCGGCAACGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 2:572763/143‑1 (MQ=255) atcagtgTCTGTTCAGTTGTCAGGGGCAAAGAAACTATGAAACTGGGCGAGCTGATGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 1:693334/137‑1 (MQ=255) tCAACGTCGTTTAAATAGCCAGACGCAAAGAAACCATGAAACGCGGCGAGCGGCTGGAAGCCCTCAAGCAAACCCACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAg < 1:290007/143‑1 (MQ=255) cGGTTCATTTGCCAGCGTCAAAGAAACCATGAATCTCGGCGAGCTGCTGGAAGCCCGCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCg < 1:657108/143‑1 (MQ=255) aaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGACTCGGGTCGAGCGACTTTc > 2:346238/1‑143 (MQ=255) aaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTAGCTGCACATTACATGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGACTGGTcgcgccagtatc > 2:350777/1‑136 (MQ=255) aaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTAAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGGATCGAGTCTGGTCGCGCGAcgggc > 2:417768/1‑139 (MQ=255) aaGAAACCATGAAACTCGGCGAGCTGCTCGACGTCGTTAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTGCCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCCAGCCTCGTCGCGCGACTGTc > 2:372246/1‑143 (MQ=255) ccATGAAACTCTGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGc < 1:191921/143‑1 (MQ=255) cATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGGTTGGTGCCGCGTAGCTGCACCTTACCAGCACCGAAGAAACACTCTGGATCCAACAGCGTATCGAGTCGGGTCGCGCGACTTTCAATAGca > 1:157931/1‑142 (MQ=255) aCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACATACTGCGGACCGATTGGTGCCGAGTATATGCACATTAACAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATAGAGTCTGGTCGCGCAACATTCAATAGCGAAgaga > 2:211191/1‑143 (MQ=255) acgccAAGCAAACCTACTGCGGCCCGATTGGGGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCCGGTCGCGCGACTTTCAAGAGCGAAGAGAAAAAACGCTTCTTAAGCGaa < 1:321034/139‑1 (MQ=255) ccTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCATATCGAGTCTGGTCGCGCGACCTTCAATAGCGAAGAGCAAAAACACTTCTTACGCGAAc > 2:408328/1‑143 (MQ=255) tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCACTAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTg > 1:84272/1‑143 (MQ=255) tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTg > 1:443368/1‑143 (MQ=255) gCACACCTACTCCGGCCCGATTGGTGCCGAGTATCTGGACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTGTCGAGTCTGGTCTCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccg < 2:324856/143‑1 (MQ=255) aaCCTACTGTGGCTCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACGTTCAATAGCGAAGATAAAAAACGCTTCTTAAGCGAACTGAccgccg < 2:319296/143‑1 (MQ=255) aCCTTCTGCTGCCTGATTGGTGCCGAGTATATGAACAAAACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGCATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACcgccgc < 1:364921/143‑1 (MQ=255) ccTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACAAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGACTGGCCGCGCGACTCTCAATAGCGCAGCGAAAAAAGGCTCCTGAAGCGAACTGACcgccgcc > 2:763810/1‑142 (MQ=255) cTGCGGCCCGGTTGGTGCTGAGTATATGCTTATTACCAGCTCAGAAGAAAAACACTGGAGCCAACAGCGTATCGAGTCGGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg < 1:212766/143‑1 (MQ=255) atGCGGCTCGATTGGTGCCGAGTACATGCACCTGACCCGCACCGAAGAAACACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg < 1:308417/142‑1 (MQ=255) tGCGGCCCGATTGGTGCCGAGTATATGAACATTACCAGCACCGAAGAAAAACTCTGGATCTAAAAGCGGATCGAGTCTGGTAGCGCGACTTTAGAGAGGGAAGAGAACACACGCAACTTAAGCCAACCGAGCGCGGCTGAAgg > 2:15021/1‑143 (MQ=255) ggccGATTGGTGCCGAGTATATGCACATTACCAGCACAGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt < 2:141197/141‑1 (MQ=255) ggTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATACCGAAGAGAAACAACGCTTCTTAAGCAAACTAACCGAAGCTGAAGGTCTTGAACGTTa > 1:683623/1‑143 (MQ=255) ggTGCCGAGCATATGCACACAACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTa < 1:559629/143‑1 (MQ=255) tGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAcc > 1:323249/1‑143 (MQ=255) ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATAGAGTCTGGTAGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGAGAACTGACAGACGCTGAAGGTATTGAAGGTTAcata > 1:450567/1‑140 (MQ=255) cGAGTACATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCg < 1:364656/143‑1 (MQ=255) tataTGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGGCTTGAACGTTACCTCGgcgc > 2:510373/1‑143 (MQ=255) cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCCCGGCGCACACTTCCCTGccg > 1:772338/1‑143 (MQ=255) gCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAATCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACATTACCCCGGCGCAAAATTcccgagcccc > 2:467123/1‑136 (MQ=255) aaaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACAGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATCGCCTGGAGCAAGACgagtcg > 2:77481/1‑138 (MQ=255) | ACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCT > NC_000913/759117‑759387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |