| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 98.0% | 142.9 / ‑5.7 | 49 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (22/26); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT > NC_000913/2132647‑2132925 | ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTc < 1:102946/143‑1 (MQ=255) ggtggtgggTAACCAGCTGTGATTTTTGGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTTATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc < 2:806109/137‑1 (MQ=255) tAGAGGGTACACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 1:39823/143‑1 (MQ=255) tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 2:962350/143‑1 (MQ=255) agagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATATTATAAACAACTTGTTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc > 1:259788/1‑143 (MQ=255) agGGTAAACAGCTGATATTTTGTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctc < 1:638550/143‑1 (MQ=255) ggTTAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt < 1:848160/143‑1 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGCTTGATCTGCAGGCTCTGCGTCGCCtctt > 1:64153/1‑143 (MQ=255) aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCATTGTGGGTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGGGGCCTCTTGTAc > 1:903909/1‑143 (MQ=255) aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCGCTTCGACtt‑t > 1:814149/1‑143 (MQ=255) tttttgGCTGGCGCTGTCGATTTTGTCTTTGTGCTTGCGGTAAAAGTGGTAGTACCCGGAGAATTGTTTCGGTGATGATGTGATCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGtt < 2:127472/143‑1 (MQ=255) ttttGCTGGCGCGGTCGCATTTGTCTTTGTGCTTGCGGTCAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCg < 1:450134/143‑1 (MQ=255) tgttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCg < 2:865587/141‑1 (MQ=255) cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTA‑CCCACGGTTCGTCGTa > 1:302188/1‑143 (MQ=255) tGGCGCGGTCGAATTTGTCTTTGTGCTTGCAGTAAAAGTGGAATTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCTCGGTTCGCCGTAc > 1:726602/1‑143 (MQ=255) ggTCGAATTTGTCTTTGTGCTTCCGGCATTAGTGGACGTACCCGGATAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTccacc < 1:257338/143‑1 (MQ=255) gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTAATCCGCATTTCACCGTGATTGATATGCCGGCCCTGCAGCCCCTCCTCTCCTT‑TCCCCCGTTCGCCGCACCccaccaccc > 2:731757/1‑142 (MQ=255) aaTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCAt > 2:229727/1‑143 (MQ=255) aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTTGTTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGCTTCGCCGTACTCCCCCACCCTc > 2:546079/1‑143 (MQ=255) gttttttTGCTTGCGGTAAGAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGTCGTACTCCACCACCATCCGCa < 2:215200/140‑1 (MQ=255) tCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTACTACTT‑TCCACGGTTCACCGTACCCCACCAACATcaccat > 1:857747/1‑138 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa < 1:1107104/143‑1 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCGTCTACTT‑TCCACCTTTCGCCCTCCTCCACCACCACCCGccaga > 1:109186/1‑139 (MQ=255) tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg > 2:618722/1‑143 (MQ=255) gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa > 1:1000009/1‑143 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCCCCCCCATCCGCCgggacacgc > 2:841981/1‑136 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCACGCTCTGCCTCGCCTCTTCTACTT‑TCCACGGTTCACCGTACTCCACCACCATCCGCAGGAAGATGt > 1:101657/1‑143 (MQ=255) tGCGGTAAACGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg < 1:194834/143‑1 (MQ=255) ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACAGTGATTGATATGCAGGCTCTGCGTCGCCTGTTCTACTT‑TCCATGGTTCAGCATACTCCACTAACATCCTAAGGGAGATGTCGGaa > 1:227417/1‑143 (MQ=255) tAAGAGTCGAGGTACGCGGCGAATTTTGTCGGTGACGCGGTGTTCTGCATTTCACCGTCATTGATCTGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc < 2:335243/143‑1 (MQ=255) cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa < 1:1069897/143‑1 (MQ=255) cccGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa < 1:753753/143‑1 (MQ=255) gagaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TGCAGGGTTCGGCTTGCTCCACCACCATCCGCAGGAAGCTGTGGTAGGACTGTGCCGCTTTCAGtgc > 2:688483/1‑141 (MQ=255) agaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCATCTACTT‑TCCACGCTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCGTTCAGTTCg > 2:574345/1‑143 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAttg > 1:191276/1‑141 (MQ=255) ttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGCACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa < 1:1047722/143‑1 (MQ=255) tCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaac < 1:618722/143‑1 (MQ=255) gggACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCGCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa < 1:197466/141‑1 (MQ=255) tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATATCCGCGATCTGATCTACTTAAACAGCAATCGTC‑ACTTTTACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGCATCGaacaaac > 1:844367/1‑143 (MQ=255) ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑GCCACGGTTAGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt < 2:814149/143‑1 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAAc > 2:924888/1‑143 (MQ=255) cactgcacaGTGCTTGCTATGCAGGCGCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCTCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGcc < 2:935149/138‑1 (MQ=255) ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCa < 2:64153/143‑1 (MQ=255) ttgattgatATTCGGGCTCTGTCTCTCCTCCTCTACTT‑TCCTCGGTTCGCCGTTCTCCACCCCCGTCCGCAGGAAGGTGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACTCTCTTTGAAACGCCATGCCCAgg < 2:776650/142‑1 (MQ=255) gTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATGCGCAGGAAAATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTCTCGAACAAACACTCTTTCAAAAGTCATGcgccgc > 2:693466/1‑138 (MQ=255) gTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGACACGCCATGCCCAgg < 1:663772/143‑1 (MQ=255) tgcttgctATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTGCACCACCTGGCGCAGGAAGATGTCGTCATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGt < 2:931036/140‑1 (MQ=255) tgatATGCAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGACATGCCCAGGTCAAg > 2:424627/1‑143 (MQ=255) cAGGCTCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt < 1:1032718/143‑1 (MQ=255) | GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTT‑TCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT > NC_000913/2132647‑2132925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |