Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,661,969 C→G V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,9690CG96.2% 142.0 / ‑6.2 55V55L (GTT→CTT) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (31/20);  minor base A (0/2);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGG  >  NC_000913/2661832‑2662103
                                                                                                                                         |                                                                                                                                      
gccGTGGCAGCCACCTTGACCCTGAAAACGGGTGGCATCTACAGAGTCGTCCACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGAc                                                                                                                                   <  1:121900/143‑1 (MQ=255)
       cAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAACGCAGACCAttttt                                                                                                                            >  2:1092704/1‑143 (MQ=255)
        aGCCGCCTTTGACCTGATAACGGGTGGCATCTACTGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTCGCATCTTTGCCTAACCGATACCCCTCCAGTTTTTCATGTACTCTGGAAAGCAGACCATTTTTa                                                                                                                           <  2:84440/143‑1 (MQ=255)
        aGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGGAAGCAGACCATTTTTa                                                                                                                           >  2:294173/1‑143 (MQ=255)
        aGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCGGGAAAGCAGACCAttttga                                                                                                                           >  2:588784/1‑141 (MQ=255)
         gccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTAc                                                                                                                          <  2:621113/143‑1 (MQ=255)
          ccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACg                                                                                                                         <  1:1110949/143‑1 (MQ=255)
          ccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGAAAACTAGACCATTTTTCCg                                                                                                                         >  1:61621/1‑143 (MQ=255)
               tctACCCTGACAACGGGCGGCAGCGACAGATTGGTCAACGGCGCTAAGTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGAc                                                                                                                    <  1:131941/141‑1 (MQ=255)
                          aaCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGGCGGGAAAACAgt                                                                                                         >  1:123412/1‑143 (MQ=255)
                                    gtcgaCAGATTCGGCAACGGCGCTAATGACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGAt                                                                                               <  1:171599/139‑1 (MQ=255)
                                    aTCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTTCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGAAAAGCAGACCATTTTTACGCAGACGGGCAAACAGTTGTTCCAGAt                                                                                               >  2:12510/1‑143 (MQ=255)
                                          aGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCCCGCACGCTGGAAAGCAGACCATCTTTACGCAGACGGGCAAACAGATGTTCCCAATAAGaaa                                                                                         >  2:747543/1‑143 (MQ=255)
                                           gATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAg                                                                                        <  2:94826/143‑1 (MQ=255)
                                           gATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTGGTTCCAGATAAGAAAg                                                                                        >  2:643875/1‑143 (MQ=255)
                                                 tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAt                                                                                  >  1:864295/1‑143 (MQ=255)
                                                 tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCCGGCATCTCTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAt                                                                                  <  1:364075/143‑1 (MQ=255)
                                                   aaCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCCTCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATACGCAAAGGAAATTc                                                                                >  2:888276/1‑143 (MQ=255)
                                                    aCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTcc                                                                               <  1:404188/143‑1 (MQ=255)
                                                     cgcctcTAATTACTTCCCCCACGTCGAGTCTGCTCGCATCATTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAGTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTccc                                                                              <  2:640955/138‑1 (MQ=255)
                                                           tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGACAACTGTTTTTCCAGATAAGAAATGGAGATTCCGTGACGt                                                                        >  1:297017/1‑143 (MQ=255)
                                                           tAATTACTTCGCCAACGGCGATGCTACTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGCCGt                                                                        >  2:673746/1‑143 (MQ=255)
                                                             aTTACTTCGCCAACGGCGATGCAGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTc                                                                      <  1:114299/143‑1 (MQ=255)
                                                                  ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGCTCGGaaa                                                                 >  1:834668/1‑143 (MQ=255)
                                                                    cGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAata                                                               >  1:661869/1‑143 (MQ=255)
                                                                     gCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAATGGGAAATTCCCTGACGTTCGGCAatat                                                              >  1:688423/1‑143 (MQ=255)
                                                                      ccAACGGCGCTGCTTCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATc                                                             <  2:281503/143‑1 (MQ=255)
                                                                      ccAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCCCCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTCTACGCCGACGGGAACACAGTCGTTCCAGCTACGAACGGGAAATTCACTGACGTTCGGAACTATc                                                             >  2:26220/1‑143 (MQ=255)
                                                                        aaCGGCGATGCTGCTGGCTTCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAg                                                           <  1:854779/143‑1 (MQ=255)
                                                                          cGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGa                                                                           <  2:671824/125‑1 (MQ=255)
                                                                          cGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGa                                                                           >  1:671824/1‑125 (MQ=255)
                                                                           ggCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCa                                                        >  2:642319/1‑143 (MQ=255)
                                                                           ggCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATAAGCCa                                                        >  2:483701/1‑143 (MQ=255)
                                                                                tgctgcTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACggc                                                   >  2:456022/1‑142 (MQ=255)
                                                                                   tgctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCCTTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTAcc                                                >  2:529516/1‑143 (MQ=255)
                                                                                          ctgtttGCCTAACAGATGACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGCAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCg                                         <  2:1001621/140‑1 (MQ=255)
                                                                                             tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCCGGAAAGAAGACCATTTTTAAGCAGACGGGAAAACAGTTCCACCAGACAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCtt                                      >  1:461828/1‑143 (MQ=255)
                                                                                                   tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTcggcgc                                >  1:127759/1‑138 (MQ=255)
                                                                                                       aGATAACCACGGCATGGTCTATGTACGCTGGAAAGCATACCATTTTTACGCTGACGGGTAAACATTTTTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAg                            <  1:492167/143‑1 (MQ=255)
                                                                                                         aTAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAACCAGATGTTCGAGATAAGAAAGGGAAATTCGCTGCCGTTCGGTAATAGCAGCACACGGTACCGCGCCGGCCTCACAGATGAacg                          >  2:910968/1‑143 (MQ=255)
                                                                                                           aaCCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCa                        >  1:560544/1‑143 (MQ=255)
                                                                                                           aaCCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGGTGAGCGCa                        >  1:560537/1‑143 (MQ=255)
                                                                                                            accaccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCaa                       >  2:49717/1‑143 (MQ=255)
                                                                                                              caccGCCTGGTCCACGTACGCTGGAAAGCAGACCATGTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAGATTGTGTGACGTTCGGAAAAAACAGCCGACGGTACCGGACCCGCGTCGGGATTGCGCGCAGCg                     >  1:897862/1‑143 (MQ=255)
                                                                                                               accGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGt                    >  1:725709/1‑143 (MQ=255)
                                                                                                                ccGCCTGGTCCACGTACGCTTGAAAGCAGACCTTTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTAGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTc                   <  1:283400/143‑1 (MQ=255)
                                                                                                                ccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGGAAACAGTTGGTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCACCGTc                   >  2:230184/1‑143 (MQ=255)
                                                                                                                  gCCTGGTCCACGTAAGCTGTAGAACAGACTATTTTTGCGCAGACGGCAAAACAGGTGTTCCAGATAAGAAAGGGAAATTCCCTTACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCaa                 <  2:209864/143‑1 (MQ=255)
                                                                                                                       gTCGACGTACGGGGGAAAGCAGACCATTGTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCAtt            <  2:329611/143‑1 (MQ=255)
                                                                                                                        tCCACGTACGCTGGAAAGCATACCTTTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg           <  2:159585/143‑1 (MQ=255)
                                                                                                                        gccATGTAAGCGGGAGAACAGACCATTTTCACGCAGATGGGAAAACAGTGGTTCCAGATAAGAAAGGGAACTTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg           <  2:592155/142‑1 (MQ=255)
                                                                                                                         ccACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCGGCTGCAGAGTTGAGCGCAACGTCAGGCATTgg          >  2:95969/1‑142 (MQ=255)
                                                                                                                                cgttGGAAATCAGACATGTTTTACGCAGACGGGAAATCAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACTGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACg   <  2:356336/140‑1 (MQ=255)
                                                                                                                                cgggggAAAGCAGAGCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACg   <  1:12510/139‑1 (MQ=255)
                                                                                                                                 gCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGCCAAGCACTGAGGTCACgg  >  2:38070/1‑143 (MQ=255)
                                                                                                                                         |                                                                                                                                      
GCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGG  >  NC_000913/2661832‑2662103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑