Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,632	T→A	V107E (GTG→GAG)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,632	0	T	A	96.8%	165.7 / NA	62	V107E (GTG→GAG)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (36/24);  minor base C (2/0);  total (38/24)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGG  >  NC_000913/3041492‑3041771                                                                                                                                             |                                                                                                                                            cgccCAGTTCGACGCCAGAGGTAAAATGGGGAGCAATGTACGTCTGTTCCGCGACGGCGAGGGCGTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAgaga                                                                                                                                           <  2:176232/143‑1 (MQ=255)    ccATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGCACGGCGCAGCGTGCGTGAACCGCAGCTGACTGCACTGAAAAACTGTGCGGTATTCTCTACAGCGACCa                                                                                                                                        >  1:354649/1‑143 (MQ=255)    ccATTGCGACGCCAAAGGGAAAATGTGGAGCAATGTACGGCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCa                                                                                                                                        <  2:386853/143‑1 (MQ=255)     cATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAAGATGCGGTATTCTCTAACGAGACCAt                                                                                                                                       >  1:1139242/1‑143 (MQ=255)        tGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTATACATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATcgc                                                                                                                                    <  1:425086/143‑1 (MQ=255)             cGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGCGGCCGTCGCGCCAg                                                                                                                               >  1:322019/1‑143 (MQ=255)              gCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGGCTGAACTGAAAAAATATGCGATATTCCCTAAAGAGACCATCGCGCCAga                                                                                                                              >  1:257530/1‑143 (MQ=255)               ccAAAGGTAAAAGGGGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgac                                                                                                                             <  1:548894/143‑1 (MQ=255)                     gTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATGTGCGGTATTCTCTAAAGAGACCATCGCGCTAGACGACGAg                                                                                                                       >  1:1007634/1‑143 (MQ=255)                       aaaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCg                                                                                                                     >  2:666402/1‑143 (MQ=255)                           tgtgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGCGCGtgtg                                                                                                                 >  1:564678/1‑143 (MQ=255)                            gtgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGtgc                                                                                                                <  2:215401/143‑1 (MQ=255)                               gAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGtgctgc                                                                                                             >  1:1105637/1‑143 (MQ=255)                                 gCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGGAtgctgcgt                                                                                                           >  2:987924/1‑141 (MQ=255)                                 gCAATCTACGTCTGTTCCGGGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCtt                                                                                                           <  1:83183/143‑1 (MQ=255)                                    aTTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAGAAATATGCGGTCTTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt                                                                                                        >  1:562161/1‑143 (MQ=255)                                    aTTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCACCAGACGACGAGCGTGTGCTGCTTGgt                                                                                                        >  2:573269/1‑143 (MQ=255)                                     tttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtg                                                                                                       <  1:972884/143‑1 (MQ=255)                                     tttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtg                                                                                                       >  1:462706/1‑143 (MQ=255)                                         cGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc                                                                                                   <  2:546073/143‑1 (MQ=255)                                           tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                                 <  1:671700/143‑1 (MQ=255)                                           tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCGTGGTGTTGCCg                                                                                                 >  2:547787/1‑143 (MQ=255)                                             tGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGCTGTTGCCAGt                                                                                               >  1:802580/1‑143 (MQ=255)                                              gTTGCGCGACGGCGATGGCGTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtt                                                                                              <  2:797450/143‑1 (MQ=255)                                              gTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtt                                                                                              >  1:76837/1‑143 (MQ=255)                                              gTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGGGAACCGCAGCGGACTGAACTGAAAAAAGATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtt                                                                                              <  1:176676/143‑1 (MQ=255)                                                   gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACCAGCGCGTGCTGCTTGGTGTTGTCGGTTTTCac                                                                                         >  1:326035/1‑142 (MQ=255)                                                      aCGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTAGTCAGgcg                                                                                      >  2:100669/1‑143 (MQ=255)                                                      aCGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGCTGTCAGgcg                                                                                      >  2:1153555/1‑143 (MQ=255)                                                           gATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc                                                                                 >  2:357131/1‑143 (MQ=255)                                                           gATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACCGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGCCGAGCGTGTGCTGCTTGGTGTTGCCGGCTTTCAGgcgcgccc                                                                                 >  2:764694/1‑141 (MQ=255)                                                           gATGGCTCTGCATGGAGTGAACGGCGCAGCGCGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc                                                                                 <  1:1016236/143‑1 (MQ=255)                                                                      aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTCTCAGGCGCGCGCCGCGCTGGCaa                                                                      >  2:477104/1‑143 (MQ=255)                                                                        ggATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt                                                                    <  1:1105671/143‑1 (MQ=255)                                                                        ggATCGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt                                                                    <  2:212867/143‑1 (MQ=255)                                                                          agtGAACGGCGCAGCGTGCGGGAGCCGCAGCGGACTGAACGGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                                                                  <  1:288855/141‑1 (MQ=255)                                                                             gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtt                                                               <  1:666402/143‑1 (MQ=255)                                                                              aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGCCGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                              >  1:109768/1‑143 (MQ=255)                                                                              aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                              <  1:1130345/143‑1 (MQ=255)                                                                                 ggCGCAGGGTTCGGGAACCGCAGCTGACTGAGTGGAACAAATATGCGGTATTCTCTACAGAGACCATCGCGCCAGGCGACGAGCGTGTTCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGc                                                           <  2:132259/143‑1 (MQ=255)                                                                                  gcgcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATACGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGGCGCGCTGGCAAATCTCTTTAGCg                                                          >  2:76879/1‑143 (MQ=255)                                                                                          tgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCAAGACGACGAGCGTGTGCAGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTCTAGAGAACTGcca                                                  >  2:182674/1‑142 (MQ=255)                                                                                           gcgtgAACCGCAGCTGACTGGACTTGAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCtt                                                 <  1:302926/143‑1 (MQ=255)                                                                                            cgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGACCTGCCtgc                                                >  1:956771/1‑141 (MQ=255)                                                                                                aaCCGCAGCTGACTGAACTTAAAAAATATTCGGTATTCTCTAAATAGACCATCGCGCTAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                            <  1:439645/143‑1 (MQ=255)                                                                                                                 ctgagAAAATATGGGGTATTCTCTAAAGAGACCAGCGCGCCAGACGGCGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaa                           <  1:518310/143‑1 (MQ=255)                                                                                                                  tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAACGAAAAACAGGTAGTCaaa                          >  2:930774/1‑143 (MQ=255)                                                                                                                    aaaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCACATCTCTTTAGCGAACTGCCTCCGAGAGAAAAACAGGGAGGCAaaga                        >  2:721091/1‑143 (MQ=255)                                                                                                                    aaaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCATGGTGTTGGCGGTTTTCAGGCGCGCGCCGCGCTGGCGAATCTCTTTAGCGAACTGCCCTCGAAAGAAAAACAGGTAGTCAaaga                        >  2:583500/1‑143 (MQ=255)                                                                                                                     aaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGAAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa                       <  1:357131/143‑1 (MQ=255)                                                                                                                     aaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAAACGACGAGCGTGTGCTGCTTGGTGTTTCCGGTCTTCAGGCGCGCGCCGCGCTGGCAAATATCCCTCGCGAACTGCCCTCGAAAGAAAAACGGGACCTCAaagca                       >  2:952955/1‑141 (MQ=255)                                                                                                                      aaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTTCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAACCAGGTAGTCCaagaag                      >  1:122448/1‑143 (MQ=255)                                                                                                                      aaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAatgaag                      >  1:975741/1‑143 (MQ=255)                                                                                                                      aaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaacaag                      >  2:762660/1‑143 (MQ=255)                                                                                                                       aaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAgg                     <  2:76837/143‑1 (MQ=255)                                                                                                                            tGCGGTATTCTCTACAGAGACCATCGCGCCGGACGACGAGCGTGTGCTGCTTGGTGTTGCCTTTTCTCAGGCGCGCGCCGCGCGGGCAAACCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa                <  1:100424/143‑1 (MQ=255)                                                                                                                              cGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGATGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGAGCGAca              >  1:577415/1‑142 (MQ=255)                                                                                                                                gTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGGCTTCGAAAGAACAACAGGTAGTCAAAGAAGGCGCGACCAc            >  1:956992/1‑143 (MQ=255)                                                                                                                                gTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTAGTGATGCCGGTTACCAGGCGCGCGCCGCGCTGGCCAATCTCTTTAGCGAACTGCCGTCGAAAAAAAAACCGGTAGTAAAGGACGGCGCGCCCAc            >  2:564186/1‑143 (MQ=255)                                                                                                                                      tctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCTAACTGCCTTCGAAAGAAAAACGGGTAGTCAAAGTATGCGAGATCACTTTGCt      >  1:585517/1‑143 (MQ=255)                                                                                                                                      tctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTCGTCAAAGAAGGCGCGACCACTTTGCt      >  1:650298/1‑143 (MQ=255)                                                                                                                                         aaaGAGACCATCGCGGCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATg   <  2:258780/143‑1 (MQ=255)                                                                                                                                          aaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAGTCTCTTTAGCGCACTGCCTCCGACAGCAACCAAATTATGTGAAGAAGCCGCGACCACTTTGCTATgc  >  2:1114003/1‑142 (MQ=255)                                                                                                                                             |                                                                                                                                            CGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGG  >  NC_000913/3041492‑3041771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑