Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,159,147	C→G	R220G (CGC→GGC)	oxyR →	DNA‑binding transcriptional dual regulator OxyR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,159,147	0	C	G	100.0%	154.8 / NA	51	R220G (CGC→GGC)	oxyR	DNA‑binding transcriptional dual regulator OxyR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (27/24);  total (27/24)

CCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGC  >  NC_000913/4159008‑4159284                                                                                                                                            |                                                                                                                                          cccTTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCggcg                                                                                                                                        <  2:328704/143‑1 (MQ=255)  ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCggcgc                                                                                                                                       >  1:960391/1‑143 (MQ=255)   cgggggCGAACCGCGAAGGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCggcgcg                                                                                                                                      <  1:988444/140‑1 (MQ=255)    gTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGa                                                                                                                                     >  1:929720/1‑143 (MQ=255)           aaCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCt                                                                                                                              <  2:98634/143‑1 (MQ=255)                cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGaaa                                                                                                                         <  1:164014/143‑1 (MQ=255)                cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCCATCAGGATATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGaaa                                                                                                                         <  1:162138/143‑1 (MQ=255)                           cGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGCGCAGGCAATGGGTTTCTGTTTTGAAGCCGAGGCGGATGAAGATAGAAACTTGGGCGCGACCAGCCTGGAACATCTGAGCCAc                                                                                                              >  2:523232/1‑143 (MQ=255)                           cGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGCAGCCGGGGCGGATGACGATACACACTTCGGCGCGACCAGCATGGAAACCATGCGCAAc                                                                                                              >  1:41585/1‑143 (MQ=255)                            gATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTCTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTACGGCGCGACCAGCCTGGAAACTCTGCGCAACa                                                                                                             >  1:859817/1‑143 (MQ=255)                               ggCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGTTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg                                                                                                          <  1:670433/143‑1 (MQ=255)                                 ccGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTGGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCAGCGCAACAtggtg                                                                                                        >  2:450335/1‑143 (MQ=255)                                     tCTGGCCGGGGAAAAGCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcgg                                                                                                    <  2:1094701/143‑1 (MQ=255)                                     tCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTCTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGACGATACACATTGCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcgg                                                                                                    <  2:1059653/143‑1 (MQ=255)                                       tgccAGGGGAAAACCTGCTGATGCTGAAAGACGACAACCGCTGTCCCGCTCAGCCAATGCGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCa                                                                                                  <  1:38724/140‑1 (MQ=255)                                       gggCAGGGGAAGACCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGACCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCa                                                                                                  <  1:98987/142‑1 (MQ=255)                                         gCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAgg                                                                                                >  1:613023/1‑143 (MQ=255)                                         gCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTCTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACGAGCGTGGCAACGCTGCGCAACGTCATGGCGGCAgg                                                                                                >  2:576175/1‑143 (MQ=255)                                         gCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTATCTGTTTTGAAGCCGGGGCGGATGAAGATACGCACTTCGGCGCGACCAGCCTGGAAACTATGCGCCACAGGGTggcgcccgg                                                                                                >  2:430683/1‑138 (MQ=255)                                           aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAGGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGGGGCGGCAGGTa                                                                                              >  1:72084/1‑143 (MQ=255)                                            ggggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGGAg                                                                                             >  1:767084/1‑143 (MQ=255)                                                aaaaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCggg                                                                                         >  1:604146/1‑143 (MQ=255)                                                    gttgctgATCTCGGAAGGTGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGTCGGATGAAGATACAGACTTCGGCGCGACCAGCCTGGAATCTCTGCGCAACATGGTGGCTGCAGGTAGCGGGATCa                                                                                     <  2:356014/141‑1 (MQ=255)                                                    cctgctgATGCTGGATGACTCCCACTCTTTGCGCCATCAGGCAATGGGTTCCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCa                                                                                     <  1:84973/142‑1 (MQ=255)                                                         tgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTa                                                                                >  1:294207/1‑143 (MQ=255)                                                            tGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACCTTACTg                                                                             <  1:1139415/143‑1 (MQ=255)                                                             ggtGGAGGATGGGCACTGTTTGCGGGATCAGGCAATGGGTTTCTGTTGTGAGGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGc                                                                            <  2:73378/141‑1 (MQ=255)                                                             gCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGc                                                                            >  1:961838/1‑143 (MQ=255)                                                                 gAAGAGGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAgc                                                                        <  1:175279/143‑1 (MQ=255)                                                                       ggTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGACTGGAAACTCTGCGCAACATGGAGGCCGCAGGTAGGGGGTTCGCTTTACTGCCAGCgctggc                                                                  >  1:579861/1‑143 (MQ=255)                                                                         tCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctggctg                                                                >  1:653237/1‑143 (MQ=255)                                                                          cACTGTTGGCGCAATCAGGCAATGGCTTTCTGTTTTGAAGCCGGGGCGGATGGAGATACACACCTCGGCGCGAGCAGCCCGGAAACTCTTTGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCtgt                                                               <  1:105117/143‑1 (MQ=255)                                                                               tttGCGCGATCAGGCAGTGGGTTTGTGTTGTGAAGCCGGGGCGGATGAAGATACACACGTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgc                                                          <  2:1027483/143‑1 (MQ=255)                                                                                   ggctctCAGGCCATGGTTTTCTCTTCTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGGCCAGCCTTGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGa                                                      <  2:859808/138‑1 (MQ=255)                                                                                     cgATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgc                                                    <  2:715635/143‑1 (MQ=255)                                                                                          aGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGACAGGTAACGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaaa                                               >  1:416230/1‑143 (MQ=255)                                                                                             cAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGAGCAGGCTGGAAACTGTGCGCAACCTGGTGGCGGCAGGTGGCGGGATCACTTGACTGCCAGCGCTGGCTGTGCAGCCGGAGGGCATAcgc                                            >  2:799794/1‑143 (MQ=255)                                                                                               aTGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGACACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTGACTGCCAGCGCTGGCTGTGCCGCCGGCGCGCACAcgcgg                                          >  2:373880/1‑142 (MQ=255)                                                                                               aTGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGa                                          >  1:201667/1‑143 (MQ=255)                                                                                                tGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGAt                                         <  1:15254/143‑1 (MQ=255)                                                                                                 gggTGTCTGTTGTGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATg                                        <  2:1096926/143‑1 (MQ=255)                                                                                                   gTTTCTGTTTTGAAGCCGGGGCGGATGAAGCTACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGGGCGCACACGCGATggg                                      >  1:10504/1‑143 (MQ=255)                                                                                                        tgtgTTCAAGCCGGCGTCGAGGAAGCTCCGCACTTCGGGGCCCCCAGCCTGGCCCCTCTGCGCCACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgttgt                                 <  1:813473/143‑1 (MQ=255)                                                                                                          ttttGAAGCCGGGGCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGttt                               >  1:544599/1‑143 (MQ=255)                                                                                                              gAAGCCGGGGCGGATGAGCATGCGCACTTCGGCGCGGCCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCt                           <  2:847564/143‑1 (MQ=255)                                                                                                                    ggggCGGAGGAAGACACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTg                     <  1:1092400/143‑1 (MQ=255)                                                                                                                     gggCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGCAGCGCAAACGCGATGGGGTTGTGGAGCTGGCGTGc                    >  1:1646/1‑143 (MQ=255)                                                                                                                       gCGGATGAAGATACACACTTCGGCGCGACCAGCCTGGAAACACTGCGAAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGGCGGAGCGCAAACGCCACAGGGCTGTGTATCGGCCGTGCag                  >  2:559391/1‑142 (MQ=255)                                                                                                                           acaaaactACACACCTCGGCGCGACCAGCCGGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAg              <  1:1000134/136‑1 (MQ=255)                                                                                                                           aTGAAGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCCGCGCCCCCCGTCCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAg              >  1:260860/1‑143 (MQ=255)                                                                                                                             gAAGTTACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGcc            <  2:1031504/143‑1 (MQ=255)                                                                                                                              aaGATACACACTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAACACTTTACTGCCAGCGCTGGCCGTGCCGCCGGAGCGCAAACGCGACGGGGTTGCTTGGCCGCCGTGGATTAAGCCg           >  2:962885/1‑143 (MQ=255)                                                                                                                                     acacTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCAc    >  1:1040084/1‑143 (MQ=255)                                                                                                                                       acTTCGGCGCGACCAGCCTGGAAACTCTGCGCAACATGTTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCgcgc  >  2:1006903/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                          CCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGC  >  NC_000913/4159008‑4159284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑