Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	97.9%	127.9 / NA	48	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (18/29);  minor base G (1/0);  total (19/29)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTA  >  NC_000913/696332‑696598                                                                                                                                           |                                                                                                                                 tCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGACTTAGaa                                                                                                                              >  2:576257/1‑143 (MQ=255)    cATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGCATGAACGCAGAAAAGCAAAAAGCGCGCCGAAGCGAGCATTTGTAATGTGGCTGCGGTACGAGGCTTCCAACCCCGGCCTCGCGGAATGAGAAAcc                                                                                                                           >  2:794997/1‑143 (MQ=255)        cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGTTATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg                                                                                                                       <  1:520835/143‑1 (MQ=255)        cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAACTAGACGCCGGTg                                                                                                                       >  2:966968/1‑143 (MQ=255)         gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc                                                                                                                      >  1:892985/1‑143 (MQ=255)          aCATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGcc                                                                                                                     <  1:1045782/143‑1 (MQ=255)             ttGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTa                                                                                                                  >  1:719000/1‑143 (MQ=255)              tGAAGGATAAGACTTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGGCGACGAGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAc                                                                                                                 <  2:1062546/143‑1 (MQ=37)              tGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTGTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAc                                                                                                                 <  1:694829/143‑1 (MQ=255)                aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg                                                                                                               >  1:103158/1‑143 (MQ=255)                   gATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCtt                                                                                                            >  1:1054014/1‑143 (MQ=255)                     tAAGACGGGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGGTTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                          <  2:113351/143‑1 (MQ=255)                          cgggtCAACATCGCATTCGAGATTGAATGAACGCAGAAGAGCAAAAGGCGCGCCGAAGCGAGCGTTTGTAGGGGGGCTGGGGGACGAGGATGCGAACGTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                     <  1:700484/140‑1 (MQ=21)                          cGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                     <  2:196041/143‑1 (MQ=255)                          cGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                     <  2:150565/143‑1 (MQ=255)                          cGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                     <  1:757436/143‑1 (MQ=255)                            tgtCACCATCGCATTCGACATTGCATGAACGCAGAAAAGCAAAACGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGTAATGCCGCAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcc                                                                                                   <  2:705780/143‑1 (MQ=25)                                aaCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa                                                                                               >  2:584303/1‑143 (MQ=255)                                      gCATTCGACAGTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                                                                                         <  2:891384/143‑1 (MQ=255)                                         ttCGACATCGAACGTCCGCCGAAAAGCAAGCAGCTCGCCGGAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                                                                                      <  2:742529/143‑1 (MQ=18)                                         atccaCATTGTATGAACGCAGAAAAGCAAAAAGCTCGCCTCAGCGAGCTTTTTTAAGGTGGCGGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                                                                                      <  2:625602/139‑1 (MQ=21)                                          tCGACATTGAATGAACGCAGAGAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAc                                                                                     <  1:656245/143‑1 (MQ=255)                                           cGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                                                                                    >  1:576822/1‑143 (MQ=255)                                                ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCAGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACat                                                                               >  2:126701/1‑142 (MQ=255)                                                      gaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGCTGCACTTCCAAGGTAAg                                                                         >  1:1064192/1‑143 (MQ=255)                                                            aGAAAATCAAAAAGCTCTCCGAAGCGAGCTTTTTTAATGTGTCTGGTGTACGAGGTTTCGTACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                   <  1:1122938/143‑1 (MQ=25)                                                            aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCAGGGTATTCCGTAGTTAGATTCCTGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                   <  1:1147882/143‑1 (MQ=37)                                                              aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa                                                                 <  1:1047176/143‑1 (MQ=255)                                                                aaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAGGCACACTTACAAGGTAAGCGTCTTGAAt                                                               >  1:363177/1‑143 (MQ=21)                                                                   cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaa                                                            <  1:911564/143‑1 (MQ=255)                                                                    aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGACTCCGGTGCCTTACCGCTTGGCGATACCCCACCTGGGCGCACTTAGCCGGCACGCGTCTAGACTAAAt                                                           >  2:774571/1‑143 (MQ=18)                                                                     aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                          >  1:819660/1‑143 (MQ=255)                                                                      aaaGCGCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTg                                                         <  1:544122/143‑1 (MQ=255)                                                                        aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGc                                                       <  2:892985/143‑1 (MQ=255)                                                                         ccgcGCCGAAGCGAGCTTTGTTACTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCt                                                      <  1:299232/140‑1 (MQ=255)                                                                               cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTACGCGTCTTGAATACATTGGCTGGGGTa                                                >  1:874324/1‑143 (MQ=255)                                                                                   gCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGCCTTGACTAAATTGGCTGGGGTACGAg                                            >  1:482604/1‑143 (MQ=255)                                                                                      aGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAt                                         <  1:436749/143‑1 (MQ=255)                                                                                          tatttAATGTGGCTGGGGTACGAGGACACGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGa                                     <  1:854194/141‑1 (MQ=255)                                                                                           ttttAATGTGGCTGGGCTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGNATAAATTGGCTGGGGTACGAGGATTCGaa                                    <  1:1113699/143‑1 (MQ=255)                                                                                                        ggcgcaCGAGAACTCGACCCTCGGAACGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGCTACCCCAACTGGGTGCACTTACAAGTTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                       <  1:596614/138‑1 (MQ=37)                                                                                                         gggTACGAGGATTTGAACCTCAGAATGGCGGAATTAGAATCCGGGGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCgg                      <  1:765308/143‑1 (MQ=37)                                                                                                           gTACGAGGATTCGACACTCGTAATGCCGGGATTAGAATCCGGGGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGAGTACGAGGATTCGAACCTCGGAATGCCGGaa                    <  2:518228/143‑1 (MQ=37)                                                                                                               gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCAGCGCCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATcgg                >  1:782711/1‑141 (MQ=255)                                                                                                                  gATTCGAACCTCGGAATGCCGGAATTAGAATCCCGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCCGCGCCTTGAAGCAACCGGCTGGGGCACGAGGCTTCGCACCGCCGAATGCCGGAGTCAGAAt             >  1:188315/1‑143 (MQ=18)                                                                                                                         aCCTCGGAATGCTGGTATTAGAATTCGGTGCCTTACTGTTTGGCGATATTCCAATTGTTTGCATTTAAAAGGTAAGCGACGTGAAAAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc      <  1:1057088/143‑1 (MQ=18)                                                                                                                           cTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTACCGAAACCCCAACTGGATGCACTTAGAAAGCAAGCGTATGGAATAAAATGGCTGGAGTACGAGGAGCGGAACCACGGAAGGCCGGAATGAGAATACGGTgggt    >  2:291382/1‑140 (MQ=16)                                                                                                                             gggAATGCCTGAATTATAATTCGGTGCCTGACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTa  <  1:775640/142‑1 (MQ=37)                                                                                                                                           |                                                                                                                                 TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTA  >  NC_000913/696332‑696598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑