| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 97.7% | 114.0 / NA | 44 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (22/20); minor base C (1/0); total (24/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTT > NC_000913/759113‑759385 | ggAGACCTTGAACGTCGGTTCAGTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGa < 1:872386/143‑1 (MQ=255) cGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTa < 1:540843/143‑1 (MQ=255) tCGGTTCATGTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCTTCAAGCATACCTACTGCGGCCCGATTGGTGACGAGTATATGAACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc < 1:192992/143‑1 (MQ=255) gatCATTTGCCAGTGGCAAAGAAACCATGAAACTAGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTAACTGCACAATACCAGCACCGAAGACAAACTCCGGAGCCAACAGCGCAACGAg > 2:26801/3‑143 (MQ=255) gTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGTTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAg > 1:796649/1‑143 (MQ=255) ttCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCAAAGAAAAACCCTGGAACCAACAGCCTACCGAgg > 2:1026707/1‑142 (MQ=255) cgttGGCAGCGGCAAAGAAACCGTGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTg < 1:362974/141‑1 (MQ=255) cGGCAAAGAAACCATGACACGCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGa < 1:167920/143‑1 (MQ=255) aGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGGATCGAGTCCGGGCGCGCGACTTTCa > 1:401371/1‑143 (MQ=255) aTGATAGTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATGGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGa < 1:197926/143‑1 (MQ=255) aaaCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAagc > 1:795819/1‑142 (MQ=255) aaCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAagag > 1:999381/1‑143 (MQ=255) cTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGGACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACGTGCACTAGCGAAGAGaa > 1:602410/1‑143 (MQ=255) gCGAGCTGCTGGAAGCCCTCAAGCAAACCTGCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaaaaa < 1:1122263/143‑1 (MQ=255) gctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttct > 1:35483/1‑143 (MQ=255) gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAg < 1:1152037/143‑1 (MQ=255) cccTCAAGCAAACCTACGGCGGCCCGAGTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGaa < 1:504210/143‑1 (MQ=255) cccTCAAGCAAACCTACCGCCGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGACTCTGGACGCGCGACTTTCTATAGCGAAGAGAAAAAACGCTTCTTAAGCGaa < 2:600883/143‑1 (MQ=255) cccTCAAGCAAACCGACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGaa < 2:1025253/143‑1 (MQ=255) cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACt > 1:806888/1‑143 (MQ=255) aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAGTGTGGATGCAACAGCGAAGCGAGGGGGGGCGCGCGACGTTGAATAGCGGAACGAAAAAAAGCGGCGTGAGCGGACTGGgcgccg > 1:456178/1‑143 (MQ=255) cTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg < 2:796649/143‑1 (MQ=255) gCCGGATTGGTGCCGAGTATATGGACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt < 1:861798/143‑1 (MQ=255) cccGGTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAACCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg < 2:748343/143‑1 (MQ=255) cccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTCCCATAGCGCAGCGGCAAACCGCTTCTTCAGCGAACTCAACGCTGCTGAAgataata > 1:870163/1‑137 (MQ=255) cGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAACAACGCTTCTCAAGCGAACTGACCGCCGCGGCAggccctgcc > 1:603325/1‑136 (MQ=255) gcttGGTGCTGGGTGTATGCACATTACCAGCGCCGAAGAACAGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGCCTTGAAc < 2:1028262/141‑1 (MQ=255) ttGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGACGAGAAAAAACGCTTCTTAAGCGAACTGACCGCGGCTGAAGGGCTGGAAcct > 2:113107/1‑141 (MQ=255) ttGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGt < 2:311438/143‑1 (MQ=255) ttGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATACAACAGCGTATCGAGTCTGGTCGCGCGACTTTCACTACCGAAGAGACAAAACGCTTCTTAAGCGAACTGCCCGCCCCTGCAGGACTTGAACGt > 2:14722/1‑143 (MQ=255) gTGCCGAGTATATGCACATTACCAGCAACGAAGGAAAAAACACGAACCAACAGCATATCGAGTAAGGTCGATCAGCTGACAATAGCGAAGACAAAAAACGCCACTTAAGCGAACTGACCGCCCCCGAAGGCCTTGAAAGGTAc > 2:1004186/1‑143 (MQ=255) gCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGCCTTGAACGTTACCt > 2:467003/1‑143 (MQ=255) cgtaGTATATGCACACTACGACGACTGCACAAAAACTCTGGATCGAACAGGGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTc < 2:780983/140‑1 (MQ=255) ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGACGCGCGACTTTCAATAGCGAAGAGAAAGAACGCGGCTTAAGCGAACTGACCGGCGCTGAAGGCAGTGAACGCTagcca > 2:1029195/1‑139 (MQ=255) atcacaTTGACAGGACCGAAGAAGAACTCTTGATTCAAAAGAGTATCGAGTCTGGTCGCTCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGGTGAAGGTTTTGAACGTTACCTCGGCGCaaaa < 2:1038517/141‑1 (MQ=255) gCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGACAAAACGCTTCTTAGGCGACCTGACCGCCGCTGAAGTGATTGAACGTTACCTGGCCGCaacgt > 2:1104424/1‑140 (MQ=255) cacaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACGTTCAATCGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAAtt > 1:373831/1‑143 (MQ=255) acaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGGAGGTCTTGAACGGTCCCTCGgcgctcacttc > 1:169383/1‑136 (MQ=255) aTTACGAGCGCCGAAGAAAAACTCCGGAACCAACAGGGTATCGAGGCTGGGCGCGCGACTGTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTccc < 1:19612/143‑1 (MQ=255) ttACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGACCGTTACCTCGGCGCAAAATTCACt > 2:799296/1‑143 (MQ=255) cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGACTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGATACCTCGGCGCAAAATTCCCTGgcg > 2:886386/1‑143 (MQ=255) aGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcgc > 2:123736/1‑143 (MQ=255) ggaCCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCTCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCa < 1:972185/141‑1 (MQ=255) gaaAAACTCTGGATCCAACAGCGTATCGAGTCAGGTCGCGCGACTTTCAATCGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCtt > 2:602202/1‑143 (MQ=255) | GGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTT > NC_000913/759113‑759385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |