| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,111,164 | G→T | R101L (CGT→CTT) | opgH → | osmoregulated periplasmic glucans (OPGs) biosynthesis protein H |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,111,164 | 0 | G | T | 94.2% | 133.8 / ‑4.2 | 54 | R101L (CGT→CTT) | opgH | osmoregulated periplasmic glucans (OPGs) biosynthesis protein H |
| Reads supporting (aligned to +/- strand): ref base G (2/0); major base T (22/27); minor base C (1/2); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGAC > NC_000913/1111026‑1111296 | tGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTc > 1:957226/1‑143 (MQ=255) ggaCAAGCCTGGCCAGGTTGACTGGCTGATGGAGAGTTGATTAAAGGCGGGGAGGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTg < 1:1089895/141‑1 (MQ=255) gatcAAGCCTGGTCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTg < 1:981477/140‑1 (MQ=255) gAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCACCTGGGTAGACCTGTTCTg > 1:151075/1‑143 (MQ=255) gAACAAGCCTGGCCAGATTCACGTGCTGATGGACAGTTAATTGAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCCCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTg < 1:1149630/143‑1 (MQ=255) aaCAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCTATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTgg > 2:706184/1‑143 (MQ=255) cAAGCCTGGCCAGCTTCCCTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGACGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGa < 2:869527/143‑1 (MQ=255) ccTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCACGATGTTTCCCGAACCGTG‑GCGTACCACCCCGGTAGGGCGTTGCTGGGATCGc > 2:323170/1‑143 (MQ=255) gCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGc > 1:562255/1‑143 (MQ=255) gCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACAAACCCGGTAGGCCTTTGCTGGGCTCGCCTGc > 2:825333/1‑143 (MQ=255) taGATTCCCGTGCTTTTGTTCAGTTATTTAAAGACGACGAATGGCGCGATCAGCTGCAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCTCGACTCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGt < 2:1028/142‑1 (MQ=255) cAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGt < 1:221120/143‑1 (MQ=255) cAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGt < 1:467523/143‑1 (MQ=255) aTTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCCGTAGACACTTTCTTGGGTGGGCTGCGTgca > 2:743856/1‑141 (MQ=255) aCTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTCCGGACCCGGTAGACGTTTTCTGGGATCGCCTGCGTGGAcgcg > 2:503155/1‑143 (MQ=255) tGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCACG‑CTATTGATCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCa > 1:1143016/1‑143 (MQ=255) aTGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCAcg < 1:911937/143‑1 (MQ=255) tGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCTACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCAcgc > 1:351092/1‑143 (MQ=255) cAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCAGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCcgcg < 1:1032360/143‑1 (MQ=255) ttaaAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTg > 2:96274/1‑143 (MQ=255) acgaAGGAGGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTg < 1:344038/143‑1 (MQ=255) cgaAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGa < 1:96274/143‑1 (MQ=255) gtgcgcTATCAGCTGGAGGCGATGCCAGAACCACAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCACCCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCaa < 2:495964/141‑1 (MQ=255) ggCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGAGCAGACGTCACGCTTCGCTATCTGGCTCGTTTGACCaca > 1:1084677/1‑141 (MQ=255) ggCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCaaa > 1:694119/1‑143 (MQ=255) gATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAagaaga < 2:268638/143‑1 (MQ=255) atcatcTGTAGGCGACGCTAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGTTAGGCCTGTCCTGAGATCGCCTGCGTGTACGCGATGTCACGCAGCGCTATCTGGCTCGTTTGACCAAATAagag < 2:983115/143‑1 (MQ=255) aGCTGAGGGCGATGGCAGAAGCAAAACGCTCCGCGATGTTTCCCGACCCGTG‑GCGGACCTACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAg < 1:34271/143‑1 (MQ=255) aGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCCCGCCCCGCTATCTGGCTCGTTTGACCACAGAACGGCAg > 2:149502/1‑143 (MQ=255) aGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTCTCTGGCTCGTTGGACCAAAGACGAGCAg > 1:650940/1‑143 (MQ=255) aGGCGATGCCAGCAGCAAAACGCTCCTCGATGTTTGCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGGCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAgagc < 2:493312/143‑1 (MQ=255) ggCGATGCCAGAAGCAACACGCTCCTGGGTGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGAGCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAgagcg < 2:327789/143‑1 (MQ=255) cGAGGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAgagcgag < 1:520736/143‑1 (MQ=255) gATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCCATCTTGCTCGTTTCGACAAAGAAGCGCAGGAaagcgagc > 2:288734/1‑143 (MQ=255) gATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCCTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAgagcgagc < 1:581546/143‑1 (MQ=255) ccAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCaaaa < 2:351092/143‑1 (MQ=255) aGAAGCAAACCGCTCCCCGCTGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGTACGCTATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGt < 1:70488/143‑1 (MQ=255) gAAGCAAAACGCTCCTCGATGTTTCCCAACCCGCA‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGtt > 1:723042/1‑142 (MQ=255) aatcGCTCCTCGTTGTTTTCTGTATTGTG‑TCGACCCAACCCGGTAGGCCTTTGCGGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTAc < 1:777128/140‑1 (MQ=255) tcctcGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAACGGCGAGCAGGCGAGCGAGCAAAAGTGGCGTACCGTCgg > 1:268447/1‑143 (MQ=255) ctcGTTGTTTCCCGGGCCGGG‑GCGTCCCGACCCGGTAGGCCTTTTCTGGGGTCGCCTGCGTGGACGCGAGGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTa < 2:933616/143‑1 (MQ=255) tGTTTCCCGACCCGGG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGGCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATc < 2:399557/143‑1 (MQ=255) tctcacGACGCGCG‑CCGTGCCACTCCGGTACGCCCGTTATGGGTTATCCTTCGTAGACGCGATGTCACGCCGCGCTATCTGTCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATccg < 2:749942/138‑1 (MQ=255) tCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATccgcc > 1:976108/1‑143 (MQ=255) tCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCGGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATccgcc < 1:687496/143‑1 (MQ=255) ccGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTGTCTGGCTCGTTTGACCCGAGAAGAGCAGGAGAGGGAGCGAAAGTGGGGTGCCGTCGGTACCAGccgccgc > 2:130222/1‑142 (MQ=255) gACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGGTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTa > 2:418276/1‑143 (MQ=255) ggg‑gCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGAGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTc < 1:212638/141‑1 (MQ=255) gCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGt > 1:1071743/1‑143 (MQ=255) gTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTg < 1:107417/143‑1 (MQ=255) ccAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGGTGCATTCTGTTGCTc > 1:144321/1‑143 (MQ=255) cAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATcc > 1:361682/1‑143 (MQ=255) aaCCCGGTAGGCCGTTTCTGGGATAGCCTGCGTGGACGCGATGTCACGCGGCGCTACCTGGGTCGTTTGCCCGAGGAAGAGCAGGAGAGCCAGCAAAAGGGACATACCGACCGTACCAGCGCCCGCTGCAGTATATTGATCCt > 2:777399/1‑143 (MQ=255) gcGGGAGGCCTTTGCGGGGCTCGCCTGCGTGGGCGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGAc < 2:752948/142‑1 (MQ=255) | TGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGAC > NC_000913/1111026‑1111296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |