| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,524,463 | Δ1 bp | 8.7% | intergenic (+95/+18) | yncE → / ← ansP | PQQ‑like domain‑containing protein YncE/L‑asparagine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,524,463 | 0 | A | . | 8.7% | 123.6 / 7.5 | 46 | intergenic (+95/+18) | yncE/ansP | PQQ‑like domain‑containing protein YncE/L‑asparagine transporter |
| Reads supporting (aligned to +/- strand): ref base A (16/25); new base . (2/2); total (18/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01 | |||||||||||
CAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGC > NC_000913/1524321‑1524601 | cagcagGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCGCGGC‑ACGTa > 1:932789/1‑143 (MQ=255) agGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCg < 1:217777/143‑1 (MQ=255) gctccgcagccagACGCTGTGCTGCGTGTTGCGCCGTAATAGGTATGGCGCGGGTGAAAACGGTGAGACCGGCCTGCCCTCTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGT‑GCCGGGCaa < 2:916552/137‑1 (MQ=255) cTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGCGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGT‑GCCGGGCaaa < 2:846784/143‑2 (MQ=255) cccAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAAtttt > 1:102881/1‑143 (MQ=255) ccagccagACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTg > 1:280864/1‑143 (MQ=255) cagccagACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGc < 1:531819/143‑1 (MQ=255) gaggctgtgATTCGTATCGCGCTGTAATAGATATGGCGAGGGTGCACACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTaa < 2:509738/138‑1 (MQ=255) aTTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCTGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAACTTTGCAATTAACTCGCCgct > 2:688545/1‑141 (MQ=255) ttCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGttt > 1:176359/1‑143 (MQ=255) cGTTTTGCGCTTTAGTAGATATGGTGAGGGTGAAAACGGTGAGACTTGCCGGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCa < 2:503782/143‑1 (MQ=255) cGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCa < 2:477378/143‑1 (MQ=255) gcTGTAATAGATGTGGCGAGGGTGAAAGCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCTGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTaaa < 2:919612/143‑1 (MQ=255) gcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTaaa < 1:523979/143‑1 (MQ=255) tAGGTATGGCGAGGGTGAAAACGGTGAGACTTGCATGCCTTTTATCACGGCGTATCCGGCACTGTCGTCAGTAATCTGAAGTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAtt < 2:793434/143‑1 (MQ=255) tAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAACCACAAtt > 1:338519/1‑143 (MQ=255) aGATATGGCGAGGGTGAAAACGGTGAGCCTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCCTGAGCACAAttt > 2:924080/1‑143 (MQ=255) aGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTGATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACAAAAttt > 1:842926/1‑143 (MQ=255) atatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGGTTCAGGCTTAAACACGATTTca > 2:476835/1‑142 (MQ=255) atGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCAGGCAAAATTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTg > 1:194570/1‑143 (MQ=255) tGGCGAGGGTGAAAACGGTGAGACTTGACTCCCCGTTATCTCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGt < 1:283963/143‑1 (MQ=255) gggTGAAAAGGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct < 1:398505/143‑1 (MQ=255) gggTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct < 2:1010942/143‑1 (MQ=255) aaaaCGGTGAGACTTGCCTGCCTTTTATCCCTGCGTATCCGGCACCTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATc < 1:1040463/143‑1 (MQ=255) gagaCTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCCTAAATACAAGTTCCTGTTTTTCTTCATCTTCCTcga < 2:977720/143‑1 (MQ=255) gcctgccgTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTgg < 2:788437/143‑1 (MQ=255) cctgcctTTTATCACGGCGTATCCGGCACTTGCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCCGTAATAGACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgc < 1:447509/143‑1 (MQ=255) ttctCACGGCGTATCCGGCACTTTCGTCAGTGATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCt < 2:648229/140‑1 (MQ=255) cACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGa > 2:941284/1‑143 (MQ=255) aCGGCGTCTCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGaa < 2:562141/143‑1 (MQ=255) gCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAAttt > 1:173470/1‑143 (MQ=255) gCGTACACGGAACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAAttt < 1:312715/143‑1 (MQ=255) tATCGGGCGCTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAg < 2:634774/143‑1 (MQ=255) cGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTACCCGGGCAATTTTGCCAATAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAAc > 1:1024752/1‑143 (MQ=255) cGGAACTTTCGAGAGTAAGCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAAc < 1:864315/143‑1 (MQ=255) ggCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACACTTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAAcg > 2:233694/1‑143 (MQ=255) cTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGtt < 2:338519/143‑1 (MQ=255) tCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCATTTTTGCAGTAAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGca < 1:1039980/143‑1 (MQ=255) tCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCCCAGTGCTGAGAATTTCAGCAACGCGTTTGCGCACACCaga > 1:291774/1‑141 (MQ=255) aaaattGCCCGGCTACGT‑GCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCCACGCGTTTGCGCCCCCCAACCCa > 1:729439/5‑143 (MQ=255) agctGCCCGGC‑ACGTAGCCGGGCAATTCTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCCACCACTTCCTCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGc < 1:245562/140‑1 (MQ=255) tGCCCGGCTACGT‑GCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACCGGCGCAGTGCTGTGAATGTCAGCAACGCGATTGCCCACACCAAACCAGccaa > 2:303522/1‑141 (MQ=255) cccGGC‑ACGTAGCCGGGCAATTTTTTTTTTTTCTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCtata > 2:40149/1‑143 (MQ=255) ccGGC‑ACGTCGCCGGGCAATTTTGCAATTCACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACTCGTTTGCGCACACCAAACCAGCCTATaa < 1:261334/143‑1 (MQ=255) ccGCC‑ACGTAGCCGGGCAAGTTTGCAATTAACTCGCCGTGTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATaa < 2:935431/143‑1 (MQ=255) c‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAg < 2:932789/143‑1 (MQ=255) aCGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGc > 2:946034/1‑143 (MQ=255) | CAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGC > NC_000913/1524321‑1524601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |