| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,622,354 | Δ1 bp | 10.0% | coding (121/639 nt) | purN → | phosphoribosylglycinamide formyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,622,354 | 0 | T | . | 10.0% | 161.1 / 14.8 | 60 | coding (121/639 nt) | purN | phosphoribosylglycinamide formyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base T (19/34); new base . (2/4); total (21/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.50e-02 | |||||||||||
CGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTT > NC_000913/2622217‑2622496 | cgctTGTTTATCGATTAAGGAATATTGTGGTGCTTATTTCCGCCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGc < 2:26578/143‑1 (MQ=255) gTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAAATATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCtt < 2:52818/143‑1 (MQ=255) gTGGTTATCGAATAATGAATATTGTGGTGCTCATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCtt < 2:12970/143‑1 (MQ=255) ggTTATCGAATAATGAATACTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCttat < 1:53244/143‑3 (MQ=255) gTTATGGAAAAATGAATATTGTGGGGTTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGaa < 2:673235/143‑1 (MQ=255) ttATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCttattg < 1:311121/143‑5 (MQ=255) tATCGAATGACTAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAAcg < 2:496079/143‑1 (MQ=255) aTCGAATAATGAATATTGGGGTGCTCATTGCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAAcgc < 1:69159/143‑1 (MQ=255) aataatGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACGAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCGTGAACGagccc > 2:1012479/1‑143 (MQ=255) aataatAAAAATGGGGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGCCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGcgccc < 1:597879/143‑1 (MQ=255) gAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGACAGTTTTCAGCCATGGGTCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAgg > 2:1018646/1‑143 (MQ=255) aaTATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGc > 2:592908/1‑143 (MQ=255) tatTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCttatt > 1:657929/1‑124 (MQ=255) tatTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCttatt < 2:657929/127‑4 (MQ=255) tatTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCgg < 1:791308/143‑1 (MQ=255) atTGTGGTGTTTATTTCGGGCAATGGAAGTAATTTACAGGCAATTATTGATGCTTGTGCAACCAACAAAATTAAAGGCAACGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCggg < 1:768534/143‑1 (MQ=255) gggtgCGTATGTCCGTCGACGGAAGGAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGTGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTAtt < 1:921062/142‑1 (MQ=255) cTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAAc > 1:540583/1‑143 (MQ=255) ttATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAAAGCGCCCGCCAGGCGGGTGTTGCAACg > 1:84791/1‑143 (MQ=255) ccGTCAACAAAAGTAGTCTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACg < 2:532432/143‑1 (MQ=255) ccGGCACTGGAAGTAATTTCCAGGCAATTATTGACGCCTGCAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACg < 2:91869/143‑1 (MQ=255) ggCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCGCGCCAGGCGGGTATTCCAACGCCTACGcc > 2:761062/1‑142 (MQ=255) ggCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCt > 1:861083/1‑143 (MQ=255) aaCGGAAGTAATGTACAGGCAATGACCGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCAt < 1:632935/143‑1 (MQ=255) tAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTGGAACGCGCCCGCCAGGCGGGCATTGCAACGCATACGCACATCGCAAgcg > 2:153129/1‑143 (MQ=255) aaTTTACAGGTAATTATTGACGCCTGTAAAACTAACAAAATTAAAGGATCCGTACTGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTTATCGCCAgcgc < 2:405346/143‑1 (MQ=255) ttACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGtt < 1:594856/143‑1 (MQ=255) cAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGa < 1:862346/143‑1 (MQ=255) ttattGGCGCCGGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGt < 2:864511/143‑1 (MQ=255) agtGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGa < 2:140630/141‑1 (MQ=255) ttGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCCCATCGCCAGCGCGTTTGACAGTCGTGaa > 1:599669/1‑143 (MQ=255) gtGACGCCTGCAAAACCAACAAAATTAAAGGCACCGTCGGGGCAGTTCTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGaa < 1:508929/142‑1 (MQ=255) cGCCTGTAAAACCAACAAAATTAAAGGCACCGAGCGTTCATTGTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCt < 1:713689/143‑1 (MQ=255) gCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTAGGCCTTGAACGAGCCCGCCAGGCGGGACTTGCAACGCATAGGACCATCGCCACCGCGTTTGCCAGCCGTGAAGccca > 2:117548/1‑141 (MQ=255) gCCTGGAAAACGAACAAAATTAAAGGGACCGTACGGGCAGTGTGCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTa < 2:120504/143‑1 (MQ=255) ccTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTGTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTAt < 1:323207/143‑1 (MQ=255) aaaaCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGCTTGACAGTCGTGAAGCCTATGACCg < 1:1029573/143‑1 (MQ=255) caCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCggg < 1:735278/142‑1 (MQ=255) aaCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCggg > 1:670094/1‑143 (MQ=255) aCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGa > 1:942468/1‑143 (MQ=255) aacaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGAAGtt > 2:779486/1‑143 (MQ=255) tAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATg < 1:576043/143‑1 (MQ=255) aGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGaaa < 1:484505/143‑1 (MQ=255) aGGCACCGTACGGGCAGGTTTCAGCAATAAGGCCG‑ACGCGTGCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGaaa < 1:107403/143‑1 (MQ=255) aCCGTGAGGGCAGCTTTCAGCAATAGGGCCG‑ACGCGTCCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGa < 2:983003/143‑1 (MQ=255) ggCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGACGCCTATGACCGGGAGGTGATTCATGAAATCGACATGTACg > 2:582424/1‑143 (MQ=255) aGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCAc < 1:807119/143‑1 (MQ=255) tCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAt < 1:1018646/143‑1 (MQ=255) gttcaaGGCCGAACGCG‑TCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGACGTCTATGACCGGGAGTTGATTCATGAAATCGACGTGTACGCACCCGATGTgg > 2:351131/5‑143 (MQ=255) ctagtgCCT‑ACCCGCTCGTCCTTGAACGCACCGGCCCGGCGCTTATTGCAACGCATACGCTCATCGGCATCGCGTTTTGCATGCGTGAAGCCTATGACCGGGAGTCGATTCATGAAATCGACATGTACGCTCCCGATGTGGTc < 2:488340/138‑1 (MQ=255) tacggCCG‑ACGCGTCCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCg < 1:934981/140‑1 (MQ=255) tAAGCCCG‑GCGCGTTTGGCCGTGATCGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCg < 1:412921/143‑1 (MQ=255) gCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGGGGGTATTGCAACGCATACGCTCATCGCCAGCGCGGTTGACAGTCGTGAAGCCTATGACCGGGAGTCGATTCATGACATCGGCCGGTACGCGCCAGCTGTGGTcatcgt > 2:560035/1‑138 (MQ=255) ccG‑ACGCGTTCGGCCTTGAACGCGCCCGCGAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgctg < 2:455539/143‑1 (MQ=255) cG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGCAGCCTATGACCGGGAGTTGACTCATGAAATCGACATGTACGCACCCGATGTGGTCCTgttgg > 1:930402/1‑143 (MQ=255) cG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCt > 2:360011/1‑86 (MQ=255) cG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCt < 1:360011/86‑1 (MQ=255) cG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgctgg < 2:683890/143‑1 (MQ=255) g‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCACCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATCTACGCACCCGATGTGGTCGTgctggc > 2:570689/1‑143 (MQ=255) gcgTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTGGGACAGTCGTGAAGCCTATGACCGGGAGTTGCTTCCTGAAATCGGCATGTACGCACACGATACGGGCGTGcgcgctgg > 2:782672/1‑143 (MQ=255) ttCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGCCATGTACGCACCCGATGTGGTCGCGCTGGCTGGctt > 1:1026816/1‑143 (MQ=255) | CGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTT > NC_000913/2622217‑2622496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |