Predicted mutation
evidence seq id position mutation freq annotation gene description
JC NC_000913 2,436,385 Δ97 bp 64.4% coding (169‑265/1014 nt) usg ← putative semialdehyde dehydrogenase Usg

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 243638421 (0.390)32 (0.610) 29/254 NT 64.4% coding (266/1014 nt) usg putative semialdehyde dehydrogenase Usg
?NC_000913 2436482 = 15 (0.290)coding (168/1014 nt) usg putative semialdehyde dehydrogenase Usg

TGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2436247‑2436384
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cctgCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTT  >  NC_000913/2436482‑2436614
                                                                                                                                                                                                                                                                               
TGGTCAGACTGTCTGGTACGGCGATGACATTCTGG‑TCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGT                                                                                                                                 <  1:484107/143‑1
            caGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCGACCTGCACGGCGATTGTCTTAC                                                                                                                      >  1:152959/3‑143
            CTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGATACGTCAGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTCCACGGTGAGTGTCTTcc                                                                                                                      >  2:143215/1‑141
               gtccgcCGATGACATTCCGGTTGCGTTGATCTGTCAGTACAAGCGGGTGTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCAC                                                                                                                   <  2:963885/137‑1
               GTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCGGGCAACCTGCACGGTGATTGTCTTACCAC                                                                                                                   <  1:683251/143‑1
                     CGATGACATTCCGGTTCCGGTACTCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGC                                                                                                             <  2:1051233/143‑1
                           CATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTGTACTGCCGGCACCACCAGCGGTACGTCCGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTT                                                                                                       <  2:847711/143‑1
                            ATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCCCCAAAGCGCAGTTG                                                                                                      >  2:211409/1‑143
                                      CGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAACGCGCAGTTGTTCGCCTGCG                                                                                            <  1:126746/143‑1
                                         TAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTT                                                                                         <  2:937171/143‑1
                                              TGTCAGTGCAACCGGGTTTACTGCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTCCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTT                                                                                    <  1:394532/143‑1
                                               GTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTA                                                                                   >  2:27720/1‑143
                                               GTCAGTACAAACGGGTGCACTTCCGGCACCACCTGCGGCACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGATCGCCTGCGCTTTCGTTA                                                                                   <  2:345294/143‑1
                                                TCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTcc                                                                                  >  1:1031872/1‑141
                                                    TACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGC                                                                              <  2:402858/143‑1
                                                        AACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGCGCAACCAATCCACTGCCGTCGATCACCAGGCAACCTGCCCGGGGATTGTCTTACCACCAAGGCGCAGAGGTTCGGCTGCGCTTTCGTTACGTctccgt                                                                          >  1:509336/1‑137
                                                        AACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCGGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCGGCGCTGCGGTTACGGGGCAGT                                                                          >  2:852885/1‑143
                                                         ACGGGTTTACGTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTG                                                                         <  1:381401/143‑1
                                                           tggccTACTTCCTGCACCACCGGCGGTACGTCGGGTTCGAGAGCAGCCATTGCCCTGCTGCCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCA                                                                       <  2:570458/138‑1
                                                                   TTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCCTCACGTGCCAGTGCTGAAAGTTC                                                               >  1:36429/1‑143
                                                                    TCCGGCACCACCAGCGGCACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCC                                                              <  1:372488/143‑1
                                                                              CCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAACGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCCACCGGGc                                                    >  2:78191/1‑142
                                                                                 GCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGGCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTCACGTGCCAGTGCATAAATTTCCCCAACCGGGAACT                                                 <  1:577496/143‑1
                                                                                    GTACGTCGGGTGCGAGAGCAAACAGTCCACTGCTGGCGAGCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGAC                                              <  2:1018581/143‑1
                                                                                             GTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTCGTTCGCCTGCGCCTTCGTTACGTGCCAGTGCATAAGTTTCGCACTACGGGCACTGACGCTCAGCCg                                     >  2:494078/1‑142
                                                                                                              CCACTGCTGTCGATCACCAGGCAACCTGCGCGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCT                    <  1:649767/143‑1
                                                                                                                 CTGCTGTCGATCGCCAGGCCCCCCTCCCGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCG                 <  2:166693/143‑1
                                                                                                                   GCTGTCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCC               <  2:548255/143‑1
                                                                                                                       TCGATCACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCCAGCAGGGCTTCGCCCACA           >  1:65008/1‑143
                                                                                                                         gcTCACCTGGCAACCTTCACGTTGATTGTCTTACCACCAAAGCTCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCTGGAACTGACGTTCAGCCAGCGTTTCTAGCAGGGCTTTGCCCACAGC         <  2:665967/141‑1
                                                                                                                             ACCAGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTACGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCA     >  1:1006814/1‑143
                                                                                                                               cgGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAGCCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGT   <  2:567407/141‑1
                                                                                                                                AGGCAACCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTT  <  1:5673/143‑1
                                                                                                                                                                                                                                                                               
TGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2436247‑2436384
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cctgCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATAAATTTCCCCAACCGGGAACTGACGTTCAGCCAGCGTTTCAAGCAGGGCTTCGCCCACAGCGCCAGTT  >  NC_000913/2436482‑2436614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.