Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 9,907 A→C 6.5% intergenic (+14/+21) mog → / ← satP molybdopterin adenylyltransferase/acetate/succinate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009139,9070AC 6.5% 148.1 / 4.2 63intergenic (+14/+21)mog/satPmolybdopterin adenylyltransferase/acetate/succinate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (33/25);  new base C (2/2);  total (36/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.64e-02

ACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGA  >  NC_000913/9770‑10046
                                                                                                                                         |                                                                                                                                           
acacGGTATTTTTGCCAGCGTGCCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCggg                                                                                                                                        <  1:614627/143‑1 (MQ=255)
   cGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCgggggg                                                                                                                                     >  2:381105/1‑143 (MQ=255)
   cGGTATTTGTCCCAGCGTACGGTCTCGCATTCAGTGGCGGGCAGGACCATACGTTGAAGCGGCACGGTGAGTGGCTGCAGCGCTTTGATCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCgggggg                                                                                                                                     <  2:973547/143‑1 (MQ=255)
    ggTATTTTTGCCAGCGGACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGa                                                                                                                                    <  1:901688/143‑1 (MQ=255)
    ggTATTTTTGAAAGCGGACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGTAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGCTCGGGGGGa                                                                                                                                    <  1:778444/143‑1 (MQ=255)
       aTTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGCTCGGGGGGAttt                                                                                                                                 <  2:286862/143‑3 (MQ=255)
        tttttGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAACATCCCCCCGAGCGGGGGGAtctc                                                                                                                                >  1:136294/1‑143 (MQ=255)
            tGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCaaaa                                                                                                                            <  1:295942/143‑1 (MQ=255)
                     accgtacTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATACCCCCGAGCTGGGGGATCTCAAAACAATTAGTg                                                                                                                   <  1:1002797/143‑1 (MQ=255)
                       cgtacTGCATTCAGTTGCTGGAAGGGCCATACGGTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTggg                                                                                                                 <  1:634806/143‑1 (MQ=255)
                         tacTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGCGCAACACACGACGCTAGCGAGTAAAAAAAGCCCCCCGAGCGGGGGGATCTCACACCAATCAGTGGGAt                                                                                                               >  2:506979/1‑143 (MQ=255)
                                  cAGTTGCTGGCAGCGCCATACGTTGAACCGGCACCGGAAGCGCTTGCAGAAATCAGCACCACAAGCGAAAGACGCCCCGTTAGCGAATAAAAAAATCAACCAGAGCGTGGGCCTCTCAAAACAATTAGTGGGATTCACCAATc                                                                                                      <  2:976950/143‑1 (MQ=255)
                                  cAGTTGCAGGAAGCGCCGTCCGTTGAAACGGCCGCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGGAAGACGGGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAACCAATTAGTGGGATTCACCAATc                                                                                                      <  2:1013039/143‑1 (MQ=255)
                                    gTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACCATTAGTGGGATCCACCAAACgg                                                                                                    >  2:820934/1‑143 (MQ=255)
                                    gTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCgg                                                                                                    >  1:62108/1‑143 (MQ=255)
                                     ttGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCGAGACGCGACGTGAGCGAATAAAAAAATCCCCCCGAGCGGGGGGAGCTCAAAAAAACTAGTGGGATTCAGCAATCGGc                                                                                                   >  1:127644/1‑143 (MQ=255)
                                       gCTGGTAGGCCCATACGGTGAAACGGCCCGGGAAGTTGGTGCAGCAATCATACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAg                                                                                                 <  2:417461/143‑1 (MQ=255)
                                             aGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGt                                                                                           >  2:993380/1‑143 (MQ=255)
                                             aGGGACAGACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGt                                                                                           <  1:615583/143‑1 (MQ=255)
                                                 ccATGCGTTGAAGCGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGa                                                                                       <  1:33223/143‑1 (MQ=255)
                                                   aTACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGCCGGAAGAGTGCAAGACGCGACGTTAGCGAGTAAGGAAATCCCCCCGGTCGGGGGGATCTCGAAACGATGAGTGGGAGTCACCAAGCGGGAGACCGGTGCgcgc                                                                                     >  2:268936/1‑140 (MQ=255)
                                                    tACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCCAGACGCGACGTTAGCGAATAAAAAAATCACCCCGAGCGGGGGGAGCTCAAAACAATTAGTGGGGTTCACCAATCGGCAGAACGGCGCGACCa                                                                                    >  2:371144/1‑143 (MQ=255)
                                                    tACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATACAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTAGGATTCACCAAACGGGAGAACGGTGCGACCa                                                                                    >  2:332001/1‑143 (MQ=255)
                                                    tACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAACCGGCAGACCGGGGCGAGCa                                                                                    >  2:908688/1‑143 (MQ=255)
                                                      gtttGAAACGGCACGGGAAGTGGTTGAAGCATTCAGACCGAAGAGTGCAAGACGGGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAGAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCaaa                                                                                  <  2:224537/141‑1 (MQ=255)
                                                      cGTTGAAACGGCGACGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCaaa                                                                                  <  2:474225/143‑1 (MQ=255)
                                                         tGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTg                                                                               <  2:119626/143‑1 (MQ=255)
                                                         tGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATACCCCCGAGCGGGGGGAAGAACAAGGAATTTGGGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTg                                                                               >  1:718246/1‑143 (MQ=255)
                                                           aaaCGGCACCGGAAGTGGTTGCAGCATTCAGACCGCAGAAGACCAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAACCGGCGCATCCCAAAACAAGTACCGGGATTCACCAACCCGCAGAACCGTGCGACCAAACTGCt                                                                             >  2:976705/1‑143 (MQ=255)
                                                            aaCGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATACAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATGCACCAATCGGCAGAACGGTGCGACCAAACTGcgc                                                                            >  1:575997/1‑141 (MQ=255)
                                                            aaCGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAACCGGCAGAACGGTGCGACCAAACTGCTc                                                                            >  2:168271/1‑143 (MQ=255)
                                                               tgCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGCCGCGACGTTAGCGAACAAAAAAATCCTCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGtt                                                                         <  1:488300/142‑1 (MQ=255)
                                                                gcgccGGAGGTGATTGCAGCATTCAGACAAAATAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGAGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTc                                                                        <  2:233572/140‑1 (MQ=255)
                                                                         gTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGGACGGTGCGACCAAACCGCCCGTTCGGTACTTcc                                                               >  2:985376/1‑142 (MQ=255)
                                                                            gTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCACACTGCTCGTGCAGTACTTCAccc                                                            >  1:404823/1‑143 (MQ=255)
                                                                             ttGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGCGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCGCGTTCAGTACTTCACCCa                                                           >  1:943416/1‑143 (MQ=255)
                                                                              cgcagccTTCCCACCGAAGGGTGCTAGACGCGGCGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACAGTGCGACCAAACTGCTCGTTCAGTACTTCACCCAt                                                          <  2:392699/142‑1 (MQ=255)
                                                                               gcagcaATCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATc                                                         <  1:997855/143‑1 (MQ=255)
                                                                                       cAGACCGACGAGTGCAAGACGCGACGTTAGCGAATAAACAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCagata                                                 <  1:876920/143‑1 (MQ=255)
                                                                                       cAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGAGGAGGGATCTCAGAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTGCAGTACTTCACCCATCGCCagata                                                 >  2:974185/1‑143 (MQ=255)
                                                                                             gAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCCGATCGATTgc                                           >  2:531229/1‑143 (MQ=255)
                                                                                                gagTGCAAGACGCGACGTTAGCGAATAAAAGCTTCGCCGCAAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCt                                        <  2:463525/143‑1 (MQ=255)
                                                                                                    gCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCACCGCCAGATAGATTGCGCTGGCa                                    >  2:915261/1‑143 (MQ=255)
                                                                                                     cAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCCATCGGCGGAACGGTGCGACCAAACTGCTAGTTCAGTACTTCACGCATCGCCAGAACGATGGCGCGGGcgc                                   >  2:436728/1‑141 (MQ=255)
                                                                                                     cAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTAAGGACTTCACCCATCGCCAGATAGCTTGGGCTGGCAc                                   >  1:252313/1‑143 (MQ=255)
                                                                                                        gtcgcgGCGTTAGCGAATAAAACAATCCCCCCGAGCGGGGGGATCTCAAACCAATGAGTGGGATTCACCAATCGGCATAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGc                                <  1:553752/141‑1 (MQ=255)
                                                                                                                gTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGc                        <  1:381105/143‑1 (MQ=255)
                                                                                                                gTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGc                        >  1:896668/1‑143 (MQ=255)
                                                                                                                    gCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGCACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCCTCGCCAGGTAGATTGCGCTGGCACCGCAGATCAGCCCac                    >  1:171264/1‑142 (MQ=255)
                                                                                                                    gCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCaa                    >  2:622614/1‑143 (MQ=255)
                                                                                                                    gCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCaa                    >  2:460656/1‑143 (MQ=255)
                                                                                                                     ggAATAATGAATTCCCCCCGAGCGGGGGGATCTCAAAACAGTTAGTGGGATTCACCAATCGGCAGCACGGTGCAACGAAACCGACCCCTCAGTCCTTCACCCATCGCCACATAGAGTGCGCTGGCACTGCAGATCAGCCCAAt                   <  2:90102/142‑1 (MQ=255)
                                                                                                                      gAATAAAAACATCCGCCCGAGCGGGGGGATCTCGAAACAATGAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATc                  <  2:342076/143‑1 (MQ=255)
                                                                                                                        aTAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCa                >  1:72502/1‑143 (MQ=255)
                                                                                                                            aaaaaTCCGCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGACTGCGCTGGCACCGCAGCTCACCCCAACCCAGCCg            >  2:701552/1‑143 (MQ=255)
                                                                                                                            aaaaaTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCg            >  1:981846/1‑143 (MQ=255)
                                                                                                                            aaaaaTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGACCAGCCCAATCCAGCCg            >  1:408613/1‑143 (MQ=255)
                                                                                                                            aaaaaTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACAGCAGATCAGCCCAATCCAGCCg            >  1:537438/1‑143 (MQ=255)
                                                                                                                               gaTCCCCCCGCTCGGGGGGATCTCAAAACAATTAGTGGGATTCACACAGCGGCAGAACGGTGCGACCAAACTACTCGTTCAGTACTCCACCAAAAGCCAAATAGATTGCGCTGACACGGCAGATCAGCCCAATCCCGGCGGCa         >  2:65489/2‑143 (MQ=255)
                                                                                                                                aTCCCCCCGCTCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCaa        >  2:566370/1‑143 (MQ=255)
                                                                                                                                    ccccGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCGCACTCCAGCCGGCCAAGTg    >  2:648003/1‑143 (MQ=255)
                                                                                                                                     cccGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCACTCCAGCCGGCAGAGTgg   >  1:745699/1‑143 (MQ=255)
                                                                                                                                      ccGAGCGGGGGGATCTCAAAACAATTAGTGGGAGTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTGCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGga  <  1:258742/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
ACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGA  >  NC_000913/9770‑10046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑