Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,900,545 A→T 7.8% A11A (GCT→GCA)  yieL ← putative hydrolase YieL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,900,5450AT 7.8% 203.0 / 7.9 74A11A (GCT→GCA) yieLputative hydrolase YieL
Reads supporting (aligned to +/- strand):  ref base A (30/37);  new base T (4/2);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.30e-01

TTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGTTT  >  NC_000913/3900403‑3900685
                                                                                                                                              |                                                                                                                                            
ttGCCCCAGTGGCAAAGGAGCGAAACGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATa                                                                                                                                              <  2:288456/143‑1 (MQ=255)
     ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGcccg                                                                                                                                         >  1:292716/1‑141 (MQ=255)
     ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACAAAATGTTTCTCCGGGATGGTGGGCGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCACTAGCCAg                                                                                                                                         >  1:489670/1‑143 (MQ=255)
      cAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGCCCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCAGGCTGGCTGTCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGc                                                                                                                                        <  1:321473/143‑1 (MQ=255)
      cAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGc                                                                                                                                        >  1:108051/1‑143 (MQ=255)
       aGGGGCAAAGTAGCGAAGGGTCACGCTGTTAGCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCTATTGCCAGCg                                                                                                                                       <  1:99993/143‑1 (MQ=255)
       aGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACAGCTGGCAATAGCCAGCg                                                                                                                                       >  2:220322/1‑143 (MQ=255)
        ggggCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGt                                                                                                                                      <  1:844502/143‑1 (MQ=255)
             aaaGTAGCGAAGGGTCACGCTGCTATCGGCATTGCCCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAag                                                                                                                                 <  2:257241/143‑1 (MQ=255)
               aGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAagca                                                                                                                               <  1:1010039/143‑1 (MQ=255)
                gTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGCTAAagcag                                                                                                                              >  2:130096/1‑143 (MQ=255)
                gTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGACCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAagcag                                                                                                                              >  2:134566/1‑143 (MQ=255)
                      agaGGGCACGCTGTTATCGGCATTGCCCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAAttt                                                                                                                        <  1:464245/141‑1 (MQ=255)
                              cGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTc                                                                                                                <  1:506198/143‑1 (MQ=255)
                                cggTTATCGGCATTGACCTGAGTCACATACTGCTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCAt                                                                                                              <  1:68570/141‑1 (MQ=255)
                                    tATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTa                                                                                                          <  1:706038/143‑1 (MQ=255)
                                     aTCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTaa                                                                                                         <  1:11640/143‑1 (MQ=255)
                                     aTCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTaa                                                                                                         <  1:157705/143‑1 (MQ=255)
                                      tCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAGTCGCCAGCGATACAGCAGCAATTTTTATATTCATCGTAat                                                                                                        >  2:788070/1‑143 (MQ=255)
                                         gCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCCGGCATATCCGCTGCTATGGCCCACTGAGCAGAGGTACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATAGTCGTCGGAACAtt                                                                                                     >  2:707901/1‑143 (MQ=255)
                                          cATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATCTCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTc                                                                                                    <  2:509954/143‑1 (MQ=255)
                                          cATGGACCTGAGTCACACACCGGCTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTc                                                                                                    <  1:255497/143‑1 (MQ=255)
                                            ttGACCTGAGTACCATACTGTTTCACCGTGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCt                                                                                                  <  1:742902/143‑1 (MQ=255)
                                                 cTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATTGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTACAGAAGCAATTTGTATATTCATAGTAATATTGCTTTCAt                                                                                             >  1:243298/1‑143 (MQ=255)
                                                    aGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTaa                                                                                          <  1:473082/143‑1 (MQ=255)
                                                             cTGTTTCACCGGCATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTa                                                                                 <  1:18116/143‑1 (MQ=255)
                                                                   gagcctgATAGCGGGTGCCGGGCTGTCTGGCATATCGCCTGCTATGGCCCCCTGAGCAGAAATATCGCTGGCAATAGCCCGCGTTACAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTAccacca                                                                           <  2:64857/137‑1 (MQ=255)
                                                                    aCCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCGTTCATTAAGTCCCATTACCACTAg                                                                          >  2:731452/1‑143 (MQ=255)
                                                                     ctgggATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGa                                                                         <  1:449935/141‑1 (MQ=255)
                                                                      cGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTGCTTTCATTAAGTTCCATTACCACCAGAt                                                                        >  2:297174/1‑143 (MQ=255)
                                                                       gggATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGAtt                                                                       <  1:444359/143‑1 (MQ=255)
                                                                           tAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAg                                                                   <  1:988509/143‑1 (MQ=255)
                                                                           tAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATCCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAg                                                                   >  2:442342/1‑143 (MQ=255)
                                                                              tGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATAGTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCt                                                                <  2:341957/143‑1 (MQ=255)
                                                                               gggTGTCGGGCTGGGTGGCAGATCCGCTGCTATGGCCCACGGAGGAGAAATACCGCTGGCAATAGCCAGCGTGAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTg                                                               <  1:602033/143‑1 (MQ=255)
                                                                                 gcccacggctggctGGCCTATCCGCTGCTATGGCCCACTGAGCAGAACTACCGCTGGCAATAGCCAGCTTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTggg                                                             <  2:476970/137‑1 (MQ=255)
                                                                                 gTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTTAGCCTtgg                                                             >  1:1034516/1‑143 (MQ=255)
                                                                                     cGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGCTTTCAGCCTGGGCAcc                                                         >  1:229204/1‑143 (MQ=255)
                                                                                     cGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCAcc                                                         <  2:865312/143‑1 (MQ=255)
                                                                                     cGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCAcc                                                         >  1:890108/1‑143 (MQ=255)
                                                                                     cGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCAcc                                                         >  1:333939/1‑143 (MQ=255)
                                                                                     cGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTCAAAGCAGCAATATTTATACTCATCGTAATACTCCTTTCCCTAAGTTCCATTCTGCCGCGAATTCAGCCTGGCCAcc                                                         >  2:413842/1‑143 (MQ=255)
                                                                                      gggCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCAGGCAATAGCCAGCGTTAAGGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGCTTTCAGACTGGGAACCg                                                        >  2:28821/1‑143 (MQ=255)
                                                                                        gctggctgGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGAc                                                      >  1:60372/1‑143 (MQ=255)
                                                                                            gctggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCACTTTTTATATTCATCGTCATATTCCTTGCCTTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGAcggaa                                                  >  2:791356/1‑139 (MQ=255)
                                                                                                gcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCCGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACCGCAAACt                                              >  1:374047/1‑143 (MQ=255)
                                                                                                gcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTCTTTTTTTGGTTGTTCTTGTTTCATTAAGATCCATTACTACCATATTTAAGTTTTGGAACAGACAGCAAACt                                              >  1:242021/1‑143 (MQ=255)
                                                                                                 cATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTg                                             <  2:493675/143‑1 (MQ=255)
                                                                                                  atatCCGCTGCTATAGCCCACTAAGAAAAAATACAGCTGGCAATAGCCAGCGTTAAAGAAGCAATTTTTATATTCATCGTAATATTCCTTTAATTAAGTTCCATTACCACCAGATTTCAGCCTGGGAACCGACAGCAAACAgg                                            >  2:77767/1‑143 (MQ=255)
                                                                                                     tCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGgtcg                                         >  1:764992/1‑143 (MQ=255)
                                                                                                     tCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTCCATTAAGCTCCATTACCACCAGCTTTCAGCCTGGGCACCGACCGCAGACCGggcg                                         >  1:303255/1‑143 (MQ=255)
                                                                                                           gctATGGCCCACTGAGCAGGAATACCGCGGTGAAGAGCCAGGCCGACAGCAGCAATCTGTATATTCATCGTAATATGCATTTCAGTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTa                                   <  1:338999/143‑1 (MQ=255)
                                                                                                           gctATGGCCCACTGAGCAGAAATCAAGCTAGTAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTa                                   <  1:192084/143‑1 (MQ=255)
                                                                                                             tATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTAtt                                 <  2:203835/143‑1 (MQ=255)
                                                                                                             tATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTAtt                                 <  1:252546/143‑1 (MQ=255)
                                                                                                             tATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTAtt                                 <  1:716633/143‑1 (MQ=255)
                                                                                                                 gCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCCGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCt                             >  2:707798/1‑143 (MQ=255)
                                                                                                                   ccACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTCTCTTCAGCTTAATATTATTTTCACGAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTc                           >  1:1048276/1‑143 (MQ=255)
                                                                                                                     aCTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCaa                         <  2:243298/143‑1 (MQ=255)
                                                                                                                      cTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCaaa                        <  2:108051/143‑1 (MQ=255)
                                                                                                                       tGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCCTTCAGTTCCATTACCCCCCGCTTTCAGCCTGGGCACCGACAGCAACCTGGTCGTCTTTATTCTCTTCaacg                       >  1:242477/1‑141 (MQ=255)
                                                                                                                       tGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTAGATTAATCGTAATATGCCTTTCATTAGGTTCCCTTACCACCAGATTTAAGCGGGGGCACGTACAGCGACCGTGTCGTCTTTAGTgtcgtcgcgg                       >  1:441093/1‑139 (MQ=255)
                                                                                                                         aGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGt                     <  1:56416/143‑1 (MQ=255)
                                                                                                                            tgatatcccGCTGGCGATAGCCAGCGTTGAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATCAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGaa                  <  2:303255/136‑1 (MQ=255)
                                                                                                                             gAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATCCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAg                 <  1:442342/143‑1 (MQ=255)
                                                                                                                              ctttaacGCTGGCTATTGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGc                >  2:465843/7‑143 (MQ=255)
                                                                                                                               tttaacGCTGGCTATTGCCAGCGGTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGcc               >  1:271377/6‑143 (MQ=255)
                                                                                                                                  aacGCTGGCTATTGCCAGCGGTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGCGAAGCCacc            >  2:563563/3‑141 (MQ=255)
                                                                                                                                   ccGCTGTCATTTGCTAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTCTCATTAAGTTCCACTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATcc           <  2:304213/143‑1 (MQ=255)
                                                                                                                                   acGCTGGCTATTGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATcc           >  1:760252/2‑143 (MQ=255)
                                                                                                                                     gCTGGCACTAGCCAGCGTTAAAGCAGCAATTTTTATATTCAGCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAg         <  1:72348/143‑1 (MQ=255)
                                                                                                                                      cTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGt        >  2:586408/1‑143 (MQ=255)
                                                                                                                                           aaTAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGtt   >  1:970325/1‑143 (MQ=255)
                                                                                                                                            aTAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGttt  <  1:134566/143‑1 (MQ=255)
                                                                                                                                            aTAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGttt  <  1:130096/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                            
TTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGTTT  >  NC_000913/3900403‑3900685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑