Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,088,091 G→C 13.1% intergenic (+34/+16) yiiG → / ← frvR DUF3829 domain‑containing lipoprotein YiiG/putative transcriptional regulator FrvR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,088,0910GC13.1% 132.6 / 13.3 53intergenic (+34/+16)yiiG/frvRDUF3829 domain‑containing lipoprotein YiiG/putative transcriptional regulator FrvR
Reads supporting (aligned to +/- strand):  ref base G (26/19);  new base C (3/4);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

TAAAGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCA  >  NC_000913/4087949‑4088227
                                                                                                                                               |                                                                                                                                        
tAAAGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGAAgcgg                                                                                                                                          >  2:180354/1‑143 (MQ=255)
    gagCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCa                                                                                                                                      >  2:394415/1‑143 (MQ=255)
    gagCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTAAGAAAGTCAATTTGTGTGGTTGCCGCCGACAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCa                                                                                                                                      <  1:244237/143‑1 (MQ=255)
         aCTTCAGGACGCAAACTCAAGCTGGGTGGTCGAAGCCTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGAGGCGCCGCATCCGa                                                                                                                                 <  1:422914/143‑1 (MQ=255)
          cTTCAGGACGCAAACTCAAGCTTGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGCCGCATCCGac                                                                                                                                <  1:709560/143‑1 (MQ=255)
           ttccgtccGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGCCGCATCCGaca                                                                                                                               <  1:138438/136‑1 (MQ=255)
              aGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGACATGC‑CAATTCGTGTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAg                                                                                                                            >  1:1042935/1‑143 (MQ=255)
                gACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAGATGCAGTGTCGGATGCGGCGCATCCGACACAGtt                                                                                                                          <  2:450223/143‑1 (MQ=255)
                   gCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCAc                                                                                                                       >  1:445934/1‑143 (MQ=255)
                        cTCGAGCTGGATGGTAGAGGACTCTTTGCCGGGGGTTTTCCCCCAGTACAACGAAGTGGTTGGTGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCtt                                                                                                                  <  2:463632/143‑1 (MQ=255)
                         tCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGCGTACAACGAAACGGTTGATGACTATAATAG‑CCTGCGTTTAACGTTTTTGATGATAAATGCAGTGTCGGATCCGGCGCATCCGACACAATTCACCCCTtg                                                                                                                 >  1:722097/1‑143 (MQ=255)
                           aaGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCCTCCGACACAGTTCACCCCTtgcg                                                                                                               >  2:36459/1‑141 (MQ=255)
                             gCTGGATGGTCGAAGACTCTTTCCGGAGAGCATTACGCGAGTACAACGAAAGGGTTGATGACTATAATAG‑CCTGCGTGAAACGTTTTTGATGAGAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGaa                                                                                                             <  1:267003/143‑1 (MQ=255)
                                 gATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGGACAACGAAATGGTTGATGACTATAATAA‑CCAGCGATAAACGATTTTGATGAAAAATAAAGTATCAGAAGCGGCGCATCCAACACAGTACACAGCTTGTGCAGTaa                                                                                                         >  2:169920/1‑143 (MQ=255)
                                    ggTCGAAGACTCTTTCCCGAGAGCATTACGCGAGGACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTc                                                                                                      <  2:826912/143‑1 (MQ=255)
                                      tCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGTCCGACACAGTTCACCCCTTGTTAAGTAACTCCa                                                                                                    >  1:460077/1‑143 (MQ=255)
                                      tCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGCGGTTGATGAGAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAACTAAcccac                                                                                                    >  2:151803/1‑139 (MQ=255)
                                         aaGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTGCATaa                                                                                                 >  1:27078/1‑143 (MQ=255)
                                            aCTCTTTCCCGAGAGCATTACGCGAGTACAAAGAAATGATCGCTGACTATAATAG‑CCTGCGTTAAACGTTTGTGATGATAGATGCAGTGTCGGAGGCGGCGCCTCGGGGACAGTTCACCGCTTGTGAAGGAACTccgtgcacg                                                                                              >  2:178470/1‑136 (MQ=255)
                                             ctctTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGc                                                                                             <  1:693645/143‑1 (MQ=255)
                                               gtttCCCGAGAGCATGACGCGAGGACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCt                                                                                           <  1:247596/142‑1 (MQ=255)
                                                tttCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTATTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCATGTGCAGTAACTCCATAAACGCCTc                                                                                          >  2:946703/1‑143 (MQ=255)
                                                  tCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCat                                                                                        >  2:429441/1‑143 (MQ=255)
                                                      gagaGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTGAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCatatgc                                                                                    >  1:201377/1‑141 (MQ=255)
                                                        gaacATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTg                                                                                  <  2:605710/140‑1 (MQ=255)
                                                            aTTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa                                                                              >  2:582292/1‑143 (MQ=255)
                                                                cgcgAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAcgtg                                                                          >  1:433834/1‑140 (MQ=255)
                                                                cgcgAGTACAACGAAATGGTTGAGGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATTCAGTGTCGTATGCGGCGCATCCGATACAGTTCACCCCTTGTGAAGTAACGCCATAAATGCCTCATATCCTGTTAACCCg                                                                          <  1:225435/143‑1 (MQ=255)
                                                                 gcgAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCgg                                                                         >  1:327619/1‑143 (MQ=255)
                                                                     gTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaat                                                                     <  1:1004072/143‑1 (MQ=255)
                                                                     gTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaat                                                                     >  1:747639/1‑143 (MQ=255)
                                                                         aaCGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGTATTAACTCCTTAAATGCCTCATATCCTGTTAACCCGGCAATAAtc                                                                 <  1:1000352/143‑1 (MQ=255)
                                                                               aTGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgcc                                                           <  2:1039259/143‑1 (MQ=255)
                                                                                        gaCTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGa                                                  >  1:197788/1‑143 (MQ=255)
                                                                                        gaCTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCAACCCTTGTGAAGTAATTCCACAAAGGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGa                                                  >  1:197116/1‑143 (MQ=255)
                                                                                               atgg‑CCTGCGTTAATCGTTTTTGATGATAAATGCAGTGTCGTATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAATTGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAata                                           <  2:393247/140‑1 (MQ=255)
                                                                                                 aG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGCTGCGACGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATg                                         <  1:336053/143‑1 (MQ=255)
                                                                                                     cTGCGTTCGTCGTTTTTGATGCTACATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGGAACTCCATAACTGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCtt                                      <  2:496324/143‑1 (MQ=255)
                                                                                                             aaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTGGTATCCTGTTAGCCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCGGATa                              >  1:517005/1‑143 (MQ=255)
                                                                                                             aaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATa                              <  2:327619/143‑1 (MQ=255)
                                                                                                                  ttttGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGCATGCTGACACAATATGCTTGCCAGATAGCtca                         >  1:995196/1‑141 (MQ=255)
                                                                                                                  ttttGATGATAAATGCAGTGGCTGATGCGGCGCATGCGACACAGTTCAGCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTaa                         <  2:700774/143‑1 (MQ=255)
                                                                                                                         gatAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTCACACAATCTGCTTGCCTGTCACCTAAattctgt                  >  1:223059/1‑138 (MQ=255)
                                                                                                                          atAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAACTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATCGCTAAATAttttt                 >  2:553828/1‑143 (MQ=255)
                                                                                                                            aaaTGCAGTGTCGGATGCGGCGCATCCGACACAGTGCACCCCTTGTGAAGTAACTCCATAAATGCCTCATAACCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAg               <  1:977371/143‑1 (MQ=255)
                                                                                                                               ggcggtgtGGGATGCGGCACATCCGACAGAGTTCACCCCTTTTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTc            <  2:989572/139‑1 (MQ=255)
                                                                                                                                 catagtCGGATGCACCAGATGCGACACAGTTCACCCCTTGTGAAGTAACTCCAGAAATGCCTAGTATCCTGTTAACCTGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCAt          <  1:139699/139‑1 (MQ=255)
                                                                                                                                  tgtgtCGGATGCGCCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCCTATCCTGTTAACCCGGCAATAATCTCCGCCGGCTGCTGACACAATATGCTTGCCGGATAGCTAAATATTTTTGGCCCacg         >  2:817683/2‑141 (MQ=255)
                                                                                                                                    tgtCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATTTAGTCCGCACACAGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATgcc       >  1:766834/1‑141 (MQ=255)
                                                                                                                                      tCGGATGCGCCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGCcgc     >  2:673997/1‑143 (MQ=255)
                                                                                                                                       cGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATCAATGCCTCATATCCTGTTCACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGAcgcg    <  2:497620/143‑1 (MQ=255)
                                                                                                                                         gATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATCAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATGTTTAGCTCATGACGCGCa  >  2:321695/1‑143 (MQ=255)
                                                                                                                                         gATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATACCCGGTGACCCCGGCAATAATCTCCGCCGGCTGCTGACACAATATGCTTGCGAGACGGCCAAATATGTTTAGCTCATggctggca  >  2:978903/1‑136 (MQ=255)
                                                                                                                                         gATGCGCCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTCGGCCATGAAgcgcg  >  1:931309/1‑142 (MQ=255)
                                                                                                                                               |                                                                                                                                        
TAAAGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAG‑CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCA  >  NC_000913/4087949‑4088227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑