| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 770,682 | A→C | 12.0% | intergenic (+71/‑776) | mngB → / → cydA | alpha‑mannosidase/cytochrome bd‑I ubiquinol oxidase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 770,682 | 0 | A | C | 12.0% | 80.1 / 4.6 | 39 | intergenic (+71/‑776) | mngB/cydA | alpha‑mannosidase/cytochrome bd‑I ubiquinol oxidase subunit I |
| Reads supporting (aligned to +/- strand): ref base A (14/20); new base C (2/3); total (16/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
AATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTT > NC_000913/770540‑770822 | aaTCAAGGTTCAAATCTACCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTCACCGGGTGGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATa < 1:241892/143‑1 (MQ=255) tCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACg > 1:265675/1‑143 (MQ=255) aaGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGc < 2:491046/143‑1 (MQ=255) gTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATg > 2:919271/1‑143 (MQ=255) ttCAAATCTATCGGGGCCTTTTCTACCCTGCCGGTGCTGTGTGCGCACTGACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCATGa < 1:235991/143‑1 (MQ=255) cccTATCCGGGACTTTTTGGCCCGGCGTGTCTCGGACGTTCGGTCACCGGCTTGCCTGAAGAGCAATCAAACCGAAGCCACGTATGCGCGGCCAGATTGGTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCATGAAcaaa < 2:410183/141‑4 (MQ=255) tCTATCGGGACCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAATCCGAAGCCACATATGCGCCGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCtt < 1:637098/143‑1 (MQ=255) ggCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGt < 2:934064/143‑1 (MQ=255) ccGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAg < 1:852267/143‑1 (MQ=255) cgcccAGTCACGGCCTTTCATTTACCCGCTTGCCTGAATAGCAAGCAAACCGCAGCCACAGAGGCGCGGCCAGATTGTTGACAGAGGGCGCTTGGTTCATGCCGGAAACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAgg < 2:871902/140‑1 (MQ=255) aCGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCaa < 2:802624/143‑1 (MQ=255) cGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATCCGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCCGCCCAATTCact > 1:882237/1‑141 (MQ=38) cGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAat > 1:359183/1‑143 (MQ=255) ggACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGCTACGGCCTGAACGCTTTTTTTGGTCTT‑CAATAGCAGGCAAATTCAata > 1:331945/1‑143 (MQ=255) ttCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTg < 1:316655/143‑1 (MQ=255) tACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAACTTCTATATATTGCAgaga > 1:36884/1‑143 (MQ=255) ttGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTa < 2:72976/143‑1 (MQ=255) aaTAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAACTCAATATATTGCAGAGATGATGTAGACACTg > 2:494596/1‑143 (MQ=255) tAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGc > 1:913692/1‑143 (MQ=255) gCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCaa < 1:919271/143‑1 (MQ=255) cAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAg > 1:595336/1‑143 (MQ=255) cAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAg > 1:242432/1‑143 (MQ=255) aaTCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTATATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGc > 2:587536/1‑143 (MQ=255) tCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCATGAAcaaa > 1:477848/1‑74 (MQ=37) tCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCATGAAcaaa < 2:477848/77‑4 (MQ=37) cGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCCGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAAGCCAATACATTGCAGAGGTGATGTAGACACTGGCAACCCTAGCGCa > 2:330837/1‑143 (MQ=255) gCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCCTTATTCGGTCTA‑CACAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAg < 1:164812/143‑1 (MQ=255) ttatGCGCGGCCAGATTGTTGACAGCGGGCGCTTGGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAGCATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAAt < 2:38888/142‑1 (MQ=255) tgttgACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCa < 2:514568/143‑1 (MQ=255) gttgACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCAc < 2:265675/143‑1 (MQ=255) aCAAAGGGCGCTTTGTTCATGCCGTATACGGCATGAACGCTTAATT‑GAACAATGGAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACtctc < 1:996725/143‑1 (MQ=37) gggCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGcc < 1:45518/143‑1 (MQ=255) gcgcTTGGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCAc < 1:364965/143‑1 (MQ=255) gcggtGTTCATGCCGGAGACGGCATGAACGCGTTATGCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGt < 2:1039290/139‑1 (MQ=255) cATGCCGGATACGGCATGAACGCTCTATTCGGCCTA‑CAAAAGCAGGCAAACTCAATATAGCGCAGAGAAGATGTAGACACTCGCAAACGTCGCGCATCAGGTAATTTTTCGTTTCTCTTCACTCTCCAGCCACGTATAtgtgc > 1:1028415/1‑142 (MQ=255) tGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCt > 2:928349/1‑143 (MQ=255) ccGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGGGCATCAGGTAATGTTGGGTTTATGTTCACTCTCAAGCCACGGATATGTGGCttt > 1:8892/1‑143 (MQ=38) cgtaTACGGCATGAACGCTTTACTCGGTCTA‑CAAAAGCAGGCAAGTTCAGTAGATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttga < 2:646683/140‑3 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATGCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCATCACTCTCAAGGCACATATACGTGGCttgaga < 2:550172/143‑5 (MQ=255) aTACGGGAGGAACGGTGTATTCGGTCTA‑CAATAGCAGGCAAATTCAATATTTTGCAGAGATGATGTAGACACTGGTAAGCGTAGCGCATCTGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAttt < 2:488287/143‑1 (MQ=255) | AATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTA‑CAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTT > NC_000913/770540‑770822 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |