| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 915,081 | G→C | 10.5% | intergenic (‑224/+271) | lysO ← / ← aqpZ | L‑lysine exporter/water channel AqpZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 915,081 | 0 | G | C | 10.5% | 112.9 / 4.8 | 45 | intergenic (‑224/+271) | lysO/aqpZ | L‑lysine exporter/water channel AqpZ |
| Reads supporting (aligned to +/- strand): ref base G (21/19); new base C (2/3); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG > NC_000913/914941‑915220 | aaaGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGAGGCGTCAAAATCGCCGACGCGTTCCCTGAAGGCCGGGGAAGCCAACATGGAt > 2:417819/1‑143 (MQ=255) gAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTgg < 1:225001/143‑1 (MQ=255) aTGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGCt < 2:604677/143‑1 (MQ=255) tGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGCTg < 1:587690/143‑1 (MQ=255) gCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGgcg > 1:701365/1‑143 (MQ=255) gCAGAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATGTTCTTGCGCTTTAATTACAATCTGCACCGATGTTGGTGGCGTCAAAATCGCAGAGGCGTTCCCTGAAGGCTGGGGCAGCCCATATGGATGTGGGCTGAGGgcg < 2:551353/143‑1 (MQ=37) cATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAg > 2:732540/1‑138 (MQ=255) cATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAg < 1:732540/138‑1 (MQ=255) cAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAg > 2:448211/1‑143 (MQ=255) aGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAgg > 1:850422/1‑143 (MQ=255) tAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGGGCTGCTTTTATGCTGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGa > 1:1043976/1‑143 (MQ=25) tAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGa > 2:273486/1‑143 (MQ=255) tAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGa > 2:203921/1‑143 (MQ=255) agagTATTCGTTTTCATTTAAAGAAATTCTTGCGCTTGAATTACAAACTGCACCGATGTTGGTGAGGTAATATGCACCAAGGCGTTCCATGAACGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGAt < 1:244828/143‑1 (MQ=21) agTATTCGTTTGCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGt < 1:967013/143‑1 (MQ=255) ttCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGCATGTCGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCgcgc < 1:400102/143‑1 (MQ=255) cATTTAAAGCTATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGccc < 1:331926/143‑1 (MQ=255) aaaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGAccc > 2:593616/1‑143 (MQ=255) aGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCgg > 1:700183/1‑143 (MQ=255) gtgttCTTGCGCTGGAATTACAAACTGCACCGATGTTGGTGGCGTCAAGATCGCCGAGGCGTTCCCTGAGGGCCGGGGCAGCCGACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTa < 2:451530/140‑1 (MQ=17) atatTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTAGAGGGATGTTACGTCGCGCCCGACCCGGTa > 1:262033/1‑143 (MQ=255) tatTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTACCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAg < 1:1045857/143‑1 (MQ=255) cgtGCGCTTTAATTACACACCGCACCGCTGGGGGGGGCGTCAAAATCGCCGAGGCGTGCCCGGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGt < 2:215300/141‑1 (MQ=14) tgccgtttCATTCCAGAGTGCACCGGTGGTGGTGGCGTCAAACTCGCCGAGGGGTCCCCTGAAGGCCGGGGCAGCCCACAGGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTaa < 2:831358/138‑1 (MQ=11) tGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTaa < 2:845538/143‑1 (MQ=255) tGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTaa < 2:547917/143‑1 (MQ=255) aaTTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAg > 2:315137/1‑143 (MQ=255) aaTTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGGAGCCGTAAGGGATAAg > 2:201998/1‑143 (MQ=255) aaccTGCACCGATGTTGGGGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACTCCGTAGCCGTAAGGGATAAGTCGAgg < 2:922632/140‑1 (MQ=255) aCTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGc > 1:180841/1‑143 (MQ=255) cTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCACTGAAGGCGGGGGGAGAGCACATGGATGTGGGAATGGAGTGGGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAggggg > 1:489752/1‑141 (MQ=14) cGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCgcgc < 1:203921/143‑1 (MQ=40) aTGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAg > 2:488847/1‑143 (MQ=38) ggtggCGTCAAAATCGCCGAGGCGGTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAgcggcg < 1:519816/143‑1 (MQ=25) tggCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAGGTGGGCTGAGGGCGCGTTTGACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATGCGTCGAGGGCACCGCGCAgcggcggt > 1:787700/1‑141 (MQ=255) tggCGTCAAAATCGCCGAGGCGTTCCCGGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGAt > 1:48447/1‑143 (MQ=25) cacaaTCGGCGAGGCGTTCCCTGCAGGCCGGGGCAGCCATCATGGATGTGGGCTGAGGGCGCGTGTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGtt < 2:479589/140‑1 (MQ=11) aaTCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGc > 1:272617/1‑143 (MQ=34) gCCGAGGCGTCCCCGGAAGGCCGGGGCAGCCGACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCaga < 1:210409/143‑1 (MQ=12) ggCGTTCGCTGAAGGCCGGGGCAGCCCACATGGAGGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCg < 1:634313/143‑1 (MQ=17) gTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCCCCAGAGccccgcc > 1:857242/1‑139 (MQ=17) ggggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTCCCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGGTAGCCGCACGCCGGACGTGCAGGGggcggc > 2:395680/1‑143 (MQ=11) ggcggcCCACATGGATGGGGCCTGAGGGCGGGTAGTACAGGGACGTTATATCGCCAAACGTCTGGTGTCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcggcgg < 1:486426/143‑1 (MQ=2) aGCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCCGCGGCGCTTTTGTGCGCCAGAGCCCGGGGGGGCGGGGGgcggcggcgc > 2:840027/1‑142 (MQ=11) aGCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGACGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGACAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGCGGGCGGCGGCGa > 2:101643/1‑143 (MQ=11) cgcagggATGGGGGCTGAGGGCGCGTTTTACAGGGATGGTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTgg < 2:448548/138‑1 (MQ=12) atggatggGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTTCAGGGGGCGGCGGCGATTGgccg < 2:642050/143‑1 (MQ=17) | AAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG > NC_000913/914941‑915220 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |