Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,159,335	G→T	6.0%	intergenic (+33/+27)	ptsG → / ← fhuE	glucose‑specific PTS enzyme IIBC component/ferric coprogen/ferric rhodotorulic acid outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,159,335	0	G	T	6.0%	163.7 / 3.9	68	intergenic (+33/+27)	ptsG/fhuE	glucose‑specific PTS enzyme IIBC component/ferric coprogen/ferric rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (30/34);  new base T (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-02

GCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAAC  >  NC_000913/1159201‑1159476                                                                                                                                       |                                                                                                                                              gcAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGc                                                                                                                                       <  2:498389/143‑1 (MQ=255)   aGCGGCCGTAGTGGTTGCTGGTTCGGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCt                                                                                                                                     <  2:281576/143‑1 (MQ=255)     cGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCttt                                                                                                                                   >  1:998970/1‑143 (MQ=255)       ggCGGAGTGGTTGCTGGTTCTGGTGTTCAGGCGAGTTTCGGTACTAAATCCGATAACCTGAAGACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCCttagt                                                                                                                                 <  2:339456/143‑4 (MQ=255)         cGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTa                                                                                                                               <  2:249712/143‑1 (MQ=255)           tAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACa                                                                                                                             <  1:102121/143‑1 (MQ=255)            aGTGGTTGCTGGTGCTGGTGTTCAGGCGATTTTCGGTACGAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTAGGGGGACTAAGGCAGCAAGGTGGCTGCCTTTTTTACAg                                                                                                                            <  2:977542/143‑1 (MQ=255)              tGGTTTCTGGATCTGGTGTTCAGGAGATCTTCTGTACTCCATCCTATCACCTTAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCTTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGgt                                                                                                                          <  2:907337/143‑1 (MQ=255)                ggtGCTGGTTCTGGGGTTCAGGCGATTTGCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAATGCAGCCAGATGGCTGCCTTTTTTACAGgtgt                                                                                                                        <  1:494278/141‑1 (MQ=255)                 ttGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGtt                                                                                                                       >  1:631456/1‑143 (MQ=255)                   gCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTCGACCCGTAACCACTAATCCGTAAGACGGTGGGGCGGCTAAGGCCGCCAGGTGGCTGCCTCTTTTGCGGGTGTTAt                                                                                                                     >  2:917634/1‑143 (MQ=255)                   gCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCACCCAGATGGCTGCCTTTTTTACAGGTGTTAt                                                                                                                     >  1:991760/1‑143 (MQ=255)                         tCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTGTTTACAGGTGATATTCAGaa                                                                                                               >  2:473624/1‑143 (MQ=255)                         tCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTCTTTACAGGTGTTATTCAGaa                                                                                                               >  2:109982/1‑143 (MQ=255)                          cTGGTGTTCAGGCGATTTTCGGTACTACATCCGATACCCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCTTTTTTTACAGGTGTTATTCAGAAt                                                                                                              <  1:152127/143‑1 (MQ=255)                           tGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAAtt                                                                                                             <  1:648661/143‑1 (MQ=255)                           tGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGGGTTGTTCAGAAtt                                                                                                             >  1:851917/1‑143 (MQ=255)                             gggtTCAGGCGATTTTCGGTACTCATGGCGATAACCTGAATACGGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCGTTTTTACAGGTGTTATTCAGAATTGa                                                                                                           <  2:908832/141‑1 (MQ=255)                              tgttgtggCGATTTTCGGTACTAATGCCGGTAATCTGAATCCCGAGATGGATGAGTACATCCGTAACCACTAAGCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGAt                                                                                                          <  2:907653/137‑1 (MQ=255)                                ttCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGGAACCACTAATCCGTAAGACGTAGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTAGAGGTGTTATTCAGAATTGATAc                                                                                                        >  2:922671/1‑143 (MQ=255)                                   aGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTg                                                                                                     >  1:580136/1‑143 (MQ=255)                                   aGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCAATAATCCGTAAGACGTTGGGGAGAATACGGCAGCCAGATGGATGCATTTTTAATCGGTGTTATACAGAATTGATACGTg                                                                                                     >  2:353299/1‑143 (MQ=255)                                      cGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCg                                                                                                  <  1:412952/143‑1 (MQ=255)                                         ttttCGGTACTAAATCCGATAACCTGAAAAACGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTAGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTATTATTCAGACGTGATACGTCCCGGTa                                                                                               >  2:226847/1‑143 (MQ=255)                                               gTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTACGACGTTGGGGAGACTACGGCAGCCAGCTAGCCCAGCTTTCTAGAGGTGATATTCAGAATTGATAGGAGCCGGTAATGCTg                                                                                         >  2:464476/1‑143 (MQ=255)                                                  cTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCTTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGaaa                                                                                      <  1:992968/143‑1 (MQ=255)                                                  cTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGaaa                                                                                      >  2:606703/1‑143 (MQ=255)                                                   tAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTACGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGATGTTATTCAGAATTGAGACGCGCCGGTAACGCTGAAAt                                                                                     >  2:161898/1‑143 (MQ=255)                                                     aacccGATAATCTGAAAGCCGAGATGGATGAGTACATCCGTAACCAGTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTa                                                                                   <  2:966815/140‑1 (MQ=255)                                                     aaTCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTa                                                                                   <  2:164458/143‑1 (MQ=255)                                                      aTCCGATATCGTGACAACGGCGATGGATGGGTTGATCCGTACCCACTAATCCGTAAGAGGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCATAGTTGATACGTGCCGGTAATGCTGAAATTAc                                                                                  <  2:270184/143‑1 (MQ=255)                                                        ccGAGAACCTGAAAAGCGAGAGGGATGAGGAACTCGGTAGTCACTAATCCGTAAGACGTTGGGGAGGCTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCATAATTGATACGTGCCGGTAATGCTGAAATTAcgc                                                                                <  1:145321/143‑1 (MQ=255)                                                           aTAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgt                                                                             <  1:860121/143‑1 (MQ=255)                                                              aCCTGAAATCCGAGATGGATGAGTACATCCGTAACCACTAGTCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTGTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGtgtg                                                                          <  2:996673/143‑1 (MQ=255)                                                               ccTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGc                                                                         <  1:675542/143‑1 (MQ=255)                                                                cTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTGGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGGGTTATACAGAATTGATACGTGCCGGTAACGCTCAACTTACACGgtcggca                                                                        >  2:442305/1‑138 (MQ=255)                                                                 tGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGACTTGATACGTGCCGGTAATGCTGCAATTACGCGGTGTGCCg                                                                       >  2:380442/1‑143 (MQ=255)                                                                     aaCCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGCCGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGa                                                                   <  1:658781/143‑1 (MQ=255)                                                                      aCCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGAc                                                                  <  1:780613/143‑1 (MQ=255)                                                                        cGAGATGGATGTGTACATCCGTAATCACTATTCTGTATGACGTTGGGGTGAGTAAGGCAGTCAGATGGGTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCTGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGa                                                                <  2:795277/143‑1 (MQ=255)                                                                         gagaTGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCGGTAGCCGAt                                                               >  2:952069/1‑143 (MQ=255)                                                                          cgatggatgAGTACATCCGTAAGCACTAAGCCGTAGGACGGTGTGGAGACTAAGGCGGCTGGGTGGCTGCCTATTTTACAGGTGTTTTGCGTAAGTTATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATa                                                              <  1:244011/142‑1 (MQ=255)                                                                              ggatgAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCGGCCTGTTTTACAGGTGTTATTCAGAATTGATCCGGGCCGGTAAAGCTGAAATTACTCGGTGTGCCGTAGAAGAGAGAAc                                                          >  2:331981/1‑143 (MQ=255)                                                                                    gaaCATGCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCa                                                    >  2:702384/3‑143 (MQ=255)                                                                                    gTACATCCGTACGCACTAATCGGGAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCTTTTTTTACAGGTGTTATGCAGAAGTGATACGGGCCGGTAATGCTGAAATTACGCGGTGTGCCGTGGACGATAGAACCTTCCa                                                    <  1:881535/143‑1 (MQ=255)                                                                                         tCCGTAACCCCTAATCCGTAAGACGTTGCGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTg                                               <  1:27466/143‑1 (MQ=255)                                                                                           cGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGt                                             >  1:949183/1‑143 (MQ=255)                                                                                                tcACTGATCCGTGAGGCGTTGGGGAGACGAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGt                                        <  2:457746/142‑1 (MQ=255)                                                                                                cccctgaTCCGTGAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGt                                        <  2:810111/137‑1 (MQ=255)                                                                                                 cACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTa                                       <  1:917634/143‑1 (MQ=255)                                                                                                 cACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCGTCCACGTTGGTATCGTa                                       >  2:835180/1‑143 (MQ=255)                                                                                                 cACTAATCCGTAAGACGTTGGGGAGACTAAAGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTa                                       >  2:863964/1‑143 (MQ=255)                                                                                                             agtcGTTGGGGAGACTAAGGCAGCCAGGTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGaa                           <  2:473461/140‑1 (MQ=255)                                                                                                                 gtcggggAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAgg                       <  1:255402/140‑1 (MQ=255)                                                                                                                 gTTGGGGAGACTAACGCAACCAGATGGCTGCCTTTTCGACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATGACGCGGTGTGCCGTAGACGATAGACCCTTCCACAGCGGTGTCGCGGGTCTTGTCGACCAgg                       >  2:426255/1‑143 (MQ=255)                                                                                                                  ttGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGTTGAAATTACGCGGTGTGACGTCGACGATAGAACCTTCCACGCTGGTACCGTAGGTTGTGTCGCACCgga                      >  2:745807/1‑142 (MQ=255)                                                                                                                    ggggggACTAAGGCAGCCAGGTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGACATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTa                    <  2:719688/143‑1 (MQ=255)                                                                                                                    ggggAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTa                    >  2:107010/1‑143 (MQ=255)                                                                                                                      ggAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATGCGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGCACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTAtt                  >  2:348349/1‑143 (MQ=255)                                                                                                                       gagaCTACGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAACTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTg                 <  1:459216/143‑1 (MQ=255)                                                                                                                        agaCTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATCGAACACCCCCCGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGa                <  1:777741/143‑1 (MQ=255)                                                                                                                         gccgaaGGCAGCCAGATGGCCGCCTTTTTTACAGGCGGTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGAc               <  1:534594/139‑1 (MQ=255)                                                                                                                           aaaaGGCAGCCATCTGGCTGCCTTTTTTACAGGCGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGt             >  1:1026294/3‑143 (MQ=255)                                                                                                                            tAAGGCAGCCAGATGGCTGCCTTTTTTACACGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATCGAACCTTCCACGTTGCTACCGCAGGCTTTCCCGACCACCTCATTGACGtc            >  2:249268/1‑142 (MQ=255)                                                                                                                               ggCAGCCATCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTccc         >  1:311326/1‑143 (MQ=255)                                                                                                                                  aGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACCTGCCGGTAATGCTGCAATTACGCGGTGTGCCGTAGGCGATAGACCCTTCCACGTTGGTCCCGTAGGTTTTGTCGCACAGGTTCTTGCCCTTccgccg      >  1:173433/1‑139 (MQ=255)                                                                                                                                    ccAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTa    >  1:1025359/1‑143 (MQ=255)                                                                                                                                      aGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAGCAGGTTATTGACGTTCCCCTGTaga  >  1:270735/1‑141 (MQ=255)                                                                                                                                       |                                                                                                                                              GCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAAC  >  NC_000913/1159201‑1159476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑