| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,233,157 | T→A | 9.1% | intergenic (‑127/+19) | dsbB ← / ← nhaB | protein thiol:quinone oxidoreductase DsbB/Na(+):H(+) antiporter NhaB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,233,157 | 0 | T | A | 9.1% | 155.1 / 7.3 | 57 | intergenic (‑127/+19) | dsbB/nhaB | protein thiol:quinone oxidoreductase DsbB/Na(+):H(+) antiporter NhaB |
| Reads supporting (aligned to +/- strand): ref base T (22/29); new base A (2/3); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01 | |||||||||||
AAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACC > NC_000913/1233019‑1233296 | aaaGCGCAGCATTGTCAGTTCCCTGCAATATGCATAGAGTGCGCAGTGGAAAACAAGTCCTTCGCTGCGCCACCAGGAGGCAGCCAAAATAGGACGCAATGGGACAATTCTCGGGCGAAAAGTCAAACCGGGCATTTATATGc < 1:47875/143‑1 (MQ=255) aTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAAGTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGccc < 1:696666/143‑1 (MQ=255) tCGCAACATATGCACTGCCCTGCAATCTGCATAGAGTGCGCAGTTGAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCg < 2:774650/143‑1 (MQ=255) tCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCg < 1:865424/143‑1 (MQ=255) cAACATATTCATTTCCCTGCATGATGCATAGAGTGCGCAGTTTACACGCGTTCATGCGCTGCGCACCCTTCAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGtt < 2:1023438/143‑2 (MQ=255) aaCATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAAATGGACAATTATCGGGCGCAAAGGCAAACCGGGCATTTATATGCCCGGTaa > 1:5419/1‑143 (MQ=255) aaCACAATCATTTCCCTGAAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGttt < 1:953744/143‑3 (MQ=255) aCATCAGCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAg < 1:902672/143‑1 (MQ=255) aCATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAg < 2:617953/143‑1 (MQ=255) tAAGCATTTCCCTGCAAGATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttg < 1:281858/143‑1 (MQ=255) tCATTTCCCTGCAATAGGCACAGAGTGCGCATTCTAAACCAATTCATCCGCTGCGCCACCAGGAGGCAGCAAAAAGAGGACGCAATTGGGCAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTa < 1:281944/143‑1 (MQ=255) tttCCCTGCAATATGCAGACAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATGGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCa < 1:869478/143‑1 (MQ=255) ttCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCaa > 2:13272/1‑143 (MQ=255) cccTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATCTATATGCCCGGTAAGATGATAGCAAAg > 2:329394/1‑143 (MQ=255) ccTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGc > 2:879513/1‑143 (MQ=255) ccTGCAATAGGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTCCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGc < 2:546946/143‑1 (MQ=255) cAATGTGCGTAGAGGTCGCAGTTTAAACCAATTCATTCGCTGCGCCACCATGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTg < 2:735928/143‑1 (MQ=255) gCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATcc < 1:850350/143‑1 (MQ=255) cgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTg < 1:35884/143‑1 (MQ=255) gcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGc > 1:680546/1‑143 (MQ=255) gcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGc > 1:841645/1‑143 (MQ=255) gTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATa > 1:935655/1‑143 (MQ=255) ttAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa < 1:348827/143‑1 (MQ=255) tAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAc < 2:39852/143‑1 (MQ=255) tAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAc < 2:842140/143‑1 (MQ=255) aCCAATTCATTCGCTGCGCCACCCGGAGGCGGAGCGAAAACGAGACAAACGGACAATTATCGGCGGAAAAGCCAAACCGCGCATGCACATGCCCGGAAAGGCGACACCAAAGCGTAGCAACCCAGCCCACCTGCAGAAACCaa > 2:126679/1‑142 (MQ=255) actcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCgg < 1:307430/141‑1 (MQ=255) attcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGCCAAATCTCGGGCGAAAAGGCAACCCGGGCATTTATATGCCCGGTACGTTGTTATCAAAGCGTTGCGAGCCAGCCCATTTGCGTAAAACATTCGGATAc > 2:391421/1‑143 (MQ=255) cGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAgg > 1:988156/1‑143 (MQ=255) gCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAggg < 1:807850/143‑1 (MQ=255) cgcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGGTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCaa < 1:454808/143‑1 (MQ=255) accaGGAGGCAGCAAAAATAGGCCGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGt < 2:1012363/143‑1 (MQ=255) gcatgcggCATCAAAAATAGGACGCAAGTGGACAATTATCGTGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTa < 2:409333/137‑1 (MQ=255) caGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTaa > 1:917570/1‑143 (MQ=255) caGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTaa > 1:985545/1‑143 (MQ=255) gaggCAGCAAATATAGGACGCAATTAGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACt < 1:518824/143‑1 (MQ=255) aggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTGAACTc > 1:542278/1‑143 (MQ=255) gcagcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGa < 1:187129/143‑1 (MQ=255) gaagtaaaaaTAGGACACAATTATACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGCTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGa < 1:90268/138‑1 (MQ=255) aaaaaTAGGACGCGATTGGGCAATTATCGGGCGAAAAGGCAGACCGGGCATTGATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCag < 2:100860/143‑1 (MQ=255) ggACGCAATTGGACAAGTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGc < 1:753699/143‑1 (MQ=255) gCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgac > 2:897488/1‑143 (MQ=255) gCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACGCGACCCAGACCCAGCcgac > 2:71480/1‑143 (MQ=255) gCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACACGACGCAGAGCAAGCcgac > 1:782162/1‑143 (MQ=255) gaaTTGAACGATTATCAGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCGAAGCGTTGCTATCCAGCGCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacg < 2:490555/142‑1 (MQ=255) aaTTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacgc > 1:42350/1‑142 (MQ=255) atcaTCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGa < 1:432292/140‑1 (MQ=255) aTTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGa < 1:432136/143‑1 (MQ=255) ggCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCCCAGCAAGCCGACGAGTGCCCGGACGAGGgt > 1:342433/1‑143 (MQ=255) gAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTACACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGGGGGTGTa > 2:589397/1‑143 (MQ=255) aaaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAATTGAATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCACGCGGACGAGTGTCAGGACGAGGGAGTaa > 2:429531/1‑143 (MQ=255) aaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATCAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCCACGAGTATCAGGCCGAGGGTGTAAg > 2:909778/1‑143 (MQ=255) cGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCAt > 1:68924/1‑143 (MQ=255) gcgCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATc < 1:364288/141‑1 (MQ=255) ggCATTTATATGCCCGGTAAGTTGTTATCAAAGCGGTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTGAGGACGAGGGAGTAAGGCAGGACCagca > 2:357235/1‑140 (MQ=255) ggCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGGGTAAGGCAGGGCCATcc > 2:822283/1‑143 (MQ=255) ttATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGGTACAGAGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTAAGGACGCGGGCGAAGGAAATCGCCATCCACAcc > 2:58958/1‑143 (MQ=255) | AAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACC > NC_000913/1233019‑1233296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |