| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 53,379 | T→C | 8.8% | E13G (GAG→GGG) | pdxA ← | 4‑hydroxythreonine‑4‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 53,379 | 0 | T | C | 8.8% | 126.9 / 5.7 | 61 | E13G (GAG→GGG) | pdxA | 4‑hydroxythreonine‑4‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (31/24); new base C (4/2); total (35/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-02 | |||||||||||
GAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATG > NC_000913/53238‑53513 | gAATATGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCGGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTc < 1:823887/143‑1 (MQ=255)gAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTc > 2:858536/1‑143 (MQ=255) aTAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGGGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGc < 1:190413/143‑1 (MQ=255) aTAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCACAGGCACAAACAAGCGGTTCGACCGGCCCCTCAACCCGGCCAAGCCGGACAACTAAGTCCGGGCCAGTCCCGGCGGGCTCGc > 2:178647/1‑143 (MQ=255) aGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGAGCCCGCCgg < 2:728280/143‑1 (MQ=255) ggcggcGGGTGAGCGGGCACCCGCGCCTCGCTCGCCGGTTGCGAAGGACAGTGGCATCGGCACAAACACCCAGTTCGACCGTCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGAGCCCGCCGGGa < 2:545492/143‑1 (MQ=255) gCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACGCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCCATCCCGGCGGGCTCGCCGGGAg > 2:400274/1‑143 (MQ=255) tGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGCGTGATccca > 2:958346/1‑143 (MQ=255) caaCCGGGCAGCGATGCTCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGCGTGATCACACCACGCTg > 2:434135/2‑143 (MQ=255) aaCCGGGCAGCGATGCTCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGGGATCACAACACGTTgg > 1:428431/1‑143 (MQ=255) aaCCGGGCAGCGATGCTCGGCTGGTAAAGAGAGTGGCATCGGCACAAACAACCAGTTCGACCAGCCACTCACGCAGCGCAAGGTGGACAACCAAGTCCGGGCCAATCCCGGCCACCCCGCCGCCAGCGAGCACAACACGtgtg > 2:757637/1‑140 (MQ=255) aCCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTggg < 2:347892/143‑1 (MQ=255) ccGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGGGTGATCACAACCCGTTGGGTTTTAACCCTTAGtt > 1:320529/1‑143 (MQ=255) gTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTGACCATTAGTTGCTCAgc > 2:8284/1‑142 (MQ=255) tAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTCGTTGCTCAggg > 1:45908/1‑142 (MQ=255) gagaGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGAtttt > 1:739058/1‑143 (MQ=255) gagaGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGAAAACTAAGTACGGGCCAATCACGGCGGGCTCGCCGGGAGTGATCACAAGACGTTGGGTTTTAACCATTAGTTGCTCAGGAtttt > 1:800328/1‑143 (MQ=255) agTGGCATCGGCACAAACCACCCGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAAc < 2:167384/143‑1 (MQ=255) tGGCATCGGCACAAACAACCAGTTCGCCCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGt > 1:468811/1‑143 (MQ=255) cATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTaaactcga > 1:758394/1‑137 (MQ=255) ggCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGAAAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGAGATCACCACACGTTGGGTTTTAACCATGAGTTGATCAGGATTTTAACGCAGGCGCt > 2:163495/1‑143 (MQ=255) gCACAAGCCACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTg < 2:30855/143‑1 (MQ=255) gCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAAAGTAGGCGCTg > 1:297939/1‑143 (MQ=255) cacaAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACGACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTgg > 2:297434/1‑143 (MQ=255) aaacaaCCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGCTTTTAACCCTTAGTTGCTCAGGATTTTAACGTAGCCGCTGGCAc > 1:721657/1‑143 (MQ=255) aaacaaCCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGACCGGGCCAATCCCGGCGGGCTCGCCGGGAATGATCACAACACGTTGGGTCTTAACCCACAGTTGCTCAGGATTTTAACCTAGGCGCTGGCAc > 2:446482/1‑143 (MQ=255) aacaacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACg < 2:719043/143‑1 (MQ=255) cAGTTGGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTc < 1:432013/143‑1 (MQ=255) ttCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTg < 1:349597/143‑1 (MQ=255) cGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCAGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCa < 1:598434/143‑1 (MQ=255) aCCGGCCAGGCACGCTGTGCGTGCGGGATGACTAGGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATc < 2:166652/143‑1 (MQ=255) cGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCGATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGGTCTTCACGTAAGCAAAGGTACGTTGTTCCCGCGTCCa > 2:681915/1‑143 (MQ=255) cGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCa < 1:858536/143‑1 (MQ=255) gCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACCCGTTGGGTTTTAACCATTAGTTGCGCAGGCTGTTAACGTCGGCCCTGGCACGTTGTTCCTGCATCCAGc > 2:338232/1‑143 (MQ=255) tcccGCTGCGCAGGCTGGACAACTAATTCCGGACCAATCCCTGCGGGCTCGCCGGGCGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTtgcg < 2:488220/140‑2 (MQ=255) gaggCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCACGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAGGGTCAGCGCAAGCAGGTTGTTTTTGTTTTGAGCTtgctg < 2:228814/140‑1 (MQ=255) cACGCTGTGCAAGCTGGACAACTCGCTCCGGGCCAGTCCCGGCGGGCTCGCCGGGAGTGATCACCACGCGTTGGGTTTTAGCCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTtgctg < 2:763255/143‑1 (MQ=255) cTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTGCCTGCATCCAGCTTGCGGcttc > 2:883870/1‑143 (MQ=255) gCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTcc < 1:223010/143‑1 (MQ=255) cTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGaa < 2:439997/143‑1 (MQ=255) tGGACAACTAAGTCCGGGCCAATCCCGGCGGGATCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGACTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAAc > 2:874356/1‑143 (MQ=255) gACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACtt > 1:180326/1‑143 (MQ=255) gACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGGTTTAACCAGCAGTTGCGCAGGATGTTAACGGAGGCGCTGGCACGTGGTGCCTGCCTGCCTCTTGCTGCCTCTTCCCAGCACtt > 2:414550/1‑143 (MQ=255) aCAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTa < 1:325329/143‑1 (MQ=255) aCTAAGTCGGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACgg < 2:794113/143‑1 (MQ=255) aCTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACgg < 1:1007652/143‑1 (MQ=255) cTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGGACTCACGGt > 1:798172/1‑143 (MQ=255) cTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGGTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCCGCATCCCGCTTGCTGCTCCTTCCGGGAACTTCCGGt > 2:336418/1‑143 (MQ=255) gTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTtcat < 1:400274/143‑1 (MQ=255) ccGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTtcatca > 1:1241/1‑143 (MQ=255) tcactCCCGGCGAGCCCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATg > 1:997739/5‑143 (MQ=255) tttCCTGGCGGGCTCGCGGGGAGTTATTACCACACGTTGGGTTTTATCCATTAGTTGCTCAGGATTTTAACGTGGGCGCTGGCACGTGGTTTCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCg < 2:189175/141‑1 (MQ=255) attCCCGGCTGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTATCCATTAGTTGCTCAGTATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCCGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCg < 2:355570/141‑1 (MQ=255) actCCCGGCGGGCTCGCCGGGAGTGCTCACAACCCGTTGGGTTTTAACCATTAGTTGCCCAGGCTTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTCCCGCGACCTTACGCTCCATCAGCATGCg > 2:275266/3‑143 (MQ=255) aaTCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCGGAGAACTTACGGTTCATCAGCATGCg > 1:301692/1‑143 (MQ=255) aTCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCGCAGGATTTTAACCTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCgg > 1:344581/1‑143 (MQ=255) tCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTGACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGGTTGCTGTGTCGTCCGAGAGCTCACGGTTCGTCAGCATGCgct > 1:193153/1‑141 (MQ=255) tCCCGGCGAGCCCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCACCATCATGCGGt > 1:367226/1‑143 (MQ=255) cccGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCTAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTa < 1:202315/143‑1 (MQ=255) ctgtcggGCTCGGCGGGAGTGATCACAACACGTTGGGTGTTAACCATTAGTTGCTCGGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGTTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTAt < 2:908120/139‑1 (MQ=255) cggcgaGCCCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATg > 1:427136/1‑143 (MQ=255) | GAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATG > NC_000913/53238‑53513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |