Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,344,036 G→C 7.0% T104S (ACT→AGT)  yciT ← putative DNA‑binding transcriptional regulator YciT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,344,0360GC 7.0% 123.6 / 3.2 53T104S (ACT→AGT) yciTputative DNA‑binding transcriptional regulator YciT
Reads supporting (aligned to +/- strand):  ref base G (21/27);  new base C (2/2);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01

TCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCA  >  NC_000913/1343896‑1344176
                                                                                                                                            |                                                                                                                                            
tCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTc                                                                                                                                            <  2:77139/143‑1 (MQ=255)
  aaaGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGGGCGACGTAGCTGCTGACCGGGATGCTAGTGGCAATCTGCTTCTGCTCGCGCAGCGTcag                                                                                                                                          >  1:836501/1‑141 (MQ=255)
  aaaGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCg                                                                                                                                          >  1:837451/1‑143 (MQ=255)
    aGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCggg                                                                                                                                        >  1:730062/1‑143 (MQ=255)
      ggCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGCTGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCCGGCg                                                                                                                                      >  1:63502/1‑143 (MQ=255)
          aaCCGTACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAgca                                                                                                                                  <  1:884359/143‑3 (MQ=255)
            ccATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATGGTGACATTTTTCTTCTGCTCGCCCAGAGGCCGGGCGAGTAgg                                                                                                                                >  1:1041896/1‑143 (MQ=255)
            ccATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAgg                                                                                                                                >  1:735723/1‑143 (MQ=255)
                 cTTTCGCTTTTTTTCTGATCCCCTCCACCTATCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGAGGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCAtt                                                                                                                           <  2:5834/143‑1 (MQ=255)
                  ctgcccTTTTTTTCTGGCACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtg                                                                                                                          <  2:842759/138‑1 (MQ=255)
                      gatttttttCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAACAGCAAGATATATCTTTTGGCCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgc                                                                                                                      >  1:395797/3‑143 (MQ=255)
                      gCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGAGGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGCCCGGGCGAGTAGGGCATtgctgc                                                                                                                      >  1:465793/1‑143 (MQ=255)
                        tttttttCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGAGGATAGTGACATTTTTCTTCTGCTCGCCCCGAGTCCGGGCGGGTCGGGCATtgctgccg                                                                                                                    >  1:203992/1‑141 (MQ=255)
                         ttttttCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctgc                                                                                                                   >  1:123031/1‑143 (MQ=255)
                                tGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                                                                            <  2:730062/143‑1 (MQ=255)
                                       cGCCACCGCGCAAAATAACCGCACCAGGCGCGTCTGTCAGCAAATGCCCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCGGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAg                                                                                                     <  2:119526/143‑1 (MQ=255)
                                       cGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAg                                                                                                     >  1:365279/1‑143 (MQ=255)
                                                   aaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGccc                                                                                         >  1:744056/1‑143 (MQ=255)
                                                   aaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACACTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGccc                                                                                         >  1:951931/1‑143 (MQ=255)
                                                    aaTAACTTCACAAGGCGCGTCCTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCg                                                                                        <  1:423745/143‑1 (MQ=255)
                                                      tAACTTCACAAGGCGCGTCTTTCAGTAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGt                                                                                      <  2:554809/143‑1 (MQ=255)
                                                          ttccgacggCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGaa                                                                                  <  2:273431/136‑1 (MQ=255)
                                                           tCACAGGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTTATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc                                                                                 <  2:395607/143‑1 (MQ=255)
                                                               aaGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGTTTTGTTTTCGGGCGAGTAGGGCAGTGCTGCTGCCATTTTCGAGAAAGATGGTTTCGCCCGGTTGAACCAGc                                                                             <  1:665265/143‑1 (MQ=255)
                                                                         tttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaa                                                                   >  1:1051404/1‑143 (MQ=255)
                                                                          ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaaa                                                                  <  1:871536/143‑1 (MQ=255)
                                                                                aaaTGCGCGATTTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc                                                            <  2:919432/143‑1 (MQ=255)
                                                                                aaaTGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCGGGGCGAGTAGGGCATGGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc                                                            <  1:75450/143‑1 (MQ=255)
                                                                                aaaTGCGCGACGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc                                                            <  1:970177/143‑1 (MQ=255)
                                                                                aaaGGCGCGTGGTAGCTGCTGACCGTGATGATGGTGCCATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCGTTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc                                                            <  2:154952/143‑1 (MQ=255)
                                                                                  aTGCGCGCTTCAGCTGCTGACGCTGTGGATAGTGATCTTTTTCTTCTGCGCGCCCAGAGTCCGGGCGGGTAGGGCACTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACTCGGCAAACTCGGCGa                                                          <  1:259309/143‑1 (MQ=255)
                                                                                  aTGCGCGATGCAGCTGCTGACCGTGATGATAGGGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGa                                                          <  1:411957/143‑1 (MQ=255)
                                                                                      gcgATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCGGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc                                                      <  2:168065/143‑1 (MQ=255)
                                                                                         gtggaGCGGCGGACCGTGAGGAGAGTGACATTTGTCTTCTGCTCGCCCAGAGGCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACg                                                   <  2:558554/139‑1 (MQ=255)
                                                                                         gtGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACg                                                   <  2:395797/142‑1 (MQ=255)
                                                                                         aTGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACg                                                   <  1:346258/143‑1 (MQ=255)
                                                                                             aGCGGCTGACCGTGATGATGGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTc                                               <  2:624032/143‑1 (MQ=255)
                                                                                               ctgctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAg                                             >  1:27436/1‑143 (MQ=255)
                                                                                                       cGTGTTGATCGTGGCGTTTTTCTTCTGCTCGCCCATAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGt                                     <  2:374936/143‑1 (MQ=255)
                                                                                                        ttgatgaTAGTTACATTTTGCTACTGCCCGCCCAGCCACCCGCCGAGTAGGGCATTTCTGCTGCCATTTTCCATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGtt                                    <  1:936514/142‑1 (MQ=255)
                                                                                                                agggACATCTTTCGTCTGCTCGCCCAGGGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCtcatca                            <  1:161605/140‑1 (MQ=255)
                                                                                                                  tGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCGCATCATa                          >  1:832276/1‑143 (MQ=255)
                                                                                                                   gACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGTGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATAc                         <  1:820259/143‑1 (MQ=255)
                                                                                                                            cttcgGCTCGCCCAGAGGCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGACAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGa                <  1:669905/143‑1 (MQ=255)
                                                                                                                             gtctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACCCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGAc               <  2:837950/142‑1 (MQ=255)
                                                                                                                              cctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCCGCCCTTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTCCGACg              >  2:325560/5‑143 (MQ=255)
                                                                                                                                tGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtc            >  1:295089/1‑143 (MQ=255)
                                                                                                                                 gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGCTGGTGTCGCCCGGTGGAACCCGCGACGCGGCAAACTCGGCGAGTTCACGCCTCAGCGTGTAGTTCCTCATCATGCGGGACTCGACGtca           >  2:50029/1‑143 (MQ=255)
                                                                                                                                 gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCGAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtca           >  1:638348/1‑143 (MQ=255)
                                                                                                                                  cTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGgcat          >  2:632564/1‑143 (MQ=255)
                                                                                                                                   tCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATATAGTCACTTTCGCCGGGTTGAAGGTAGAATACAACAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtcctc         >  1:772217/1‑143 (MQ=255)
                                                                                                                                    cggtcAGGGTGCGGGGGAGTAGGGCTGTGCTGCTGCCATTTTCGTTAAAGAGGGTTGCGCGCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtcatca        <  2:450862/139‑1 (MQ=255)
                                                                                                                                       ccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGGTCACGCTTCAGCGTGTAGTTGCGCATCATACGGGTGTCGACGTCATGACTa     >  1:815502/1‑143 (MQ=255)
                                                                                                                                          gagTCCGTGCGAGTCTCGCATTGCTGCTCCCATCTTCGATAACGCTGTTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCCCTATCa  <  2:805399/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCA  >  NC_000913/1343896‑1344176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑