| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,557,194 | A→C | 12.2% | L282R (CTG→CGG) | ddpF ← | putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,557,194 | 0 | A | C | 12.2% | 117.9 / 11.0 | 55 | L282R (CTG→CGG) | ddpF | putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpF |
| Reads supporting (aligned to +/- strand): ref base A (25/21); new base C (4/3); total (31/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-02 | |||||||||||
AAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTAT > NC_000913/1557056‑1557328 | aaGCATTGACGGAGGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGGGAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGCGGGCTGCCAGccg > 2:1032103/1‑141 (MQ=255) ttGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCTGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCCGCTGGCAAc < 2:952556/143‑1 (MQ=255) tGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCCGCTGGCAAcc < 2:308600/143‑2 (MQ=255) ccGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTAATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGACAGCGGGCAACGtt > 2:99612/1‑143 (MQ=255) ggATGCCGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCCGCTGGCAAcccacg < 1:1050061/143‑6 (MQ=255) aTGTCGCGTCATGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTa < 2:291642/143‑1 (MQ=255) gcgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAGCGTGCGTAGaaaa > 2:286054/1‑143 (MQ=255) gcgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCGCACGTCCGTCCTCCCTTAGTGCTAATGATTGCCCGACGTCACATCCGTGGGTTGCCAGCGGGCACCGCTCGTAGcaac > 1:422972/1‑142 (MQ=255) cgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGGTGACGTCCTTCCTCCCTTATTGCTAATGATTGCCGGACTTCCCATCCGTGGGTGGCCGCCGGGCAACGTTCGTAGaaaca > 1:943359/1‑141 (MQ=255) gTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCACCTCTCCGCCAGCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGaaaaaa < 1:336054/143‑1 (MQ=255) aGGCGCGTGAATGCCTGATGCGTTGTTAGCAGCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAg < 1:979484/143‑1 (MQ=255) gATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGc > 1:683182/1‑143 (MQ=255) tGCGGTGTTAGCACCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGGCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCgg < 1:446970/143‑1 (MQ=255) gttgttAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGATGCCCGCCGGCACCGTTCGTCGACCAAACAGCCATCCGGCAAAGTCCGCttg > 2:330726/1‑142 (MQ=255) tgttAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAACGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTcc < 1:529818/143‑1 (MQ=255) tAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCgg < 1:930214/143‑1 (MQ=255) tctACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCACCGTGCGCATTCCCGGCAGATcc > 1:964663/1‑143 (MQ=255) tACAGCGCCCGCCAGCACTGGAGCTCACTTCCGTCCTCCCGTGTGCCTAAGGATCGCGGGCCTTCACAGCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGt < 1:981004/143‑1 (MQ=255) ccacgcccgccAGCCGCTGAGCTCACGCCCTTCCTCTCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGttt < 2:105062/140‑1 (MQ=255) cAGGGCGCGCAAGCGCCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGttt < 2:162239/143‑1 (MQ=255) cAGCGCCCGCCAGCACCGGCCCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACGTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGACGAAGCAGCCTTGCGACAACGTCCGGTCTCCCGGCAGATCCGttt > 2:1036178/1‑143 (MQ=255) gcgcCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGCACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTa < 2:221164/143‑1 (MQ=255) gcccgccAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCAGCAGATCCGTTTTACg > 1:128161/1‑143 (MQ=255) gcccgccAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGGGCGGTTTCCCGGCAGATCCGTTCTACg > 2:168212/1‑143 (MQ=255) ccgccAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTa > 1:866096/1‑143 (MQ=255) ccgccAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTa > 1:919124/1‑143 (MQ=255) gccAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTact > 1:918738/1‑141 (MQ=255) cAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCCGATCCGTTTTACGTAATgg > 1:861519/1‑142 (MQ=255) cGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTCCGTAATGCCCAttc > 2:994256/1‑143 (MQ=255) gAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCCCATAAGTGGCATGTAAGCGAGCAACATTCGTAGGCAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGCTTTACGTAATGCCCAttctt > 1:539479/1‑143 (MQ=255) aGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCAttcttc < 2:755812/143‑1 (MQ=255) aGCTCACGTCCGTCCGCCCTTATGGCTAATGAGTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCAttcttc < 1:71871/143‑1 (MQ=255) cACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCCGATCCGCTTTCCGTCCTGCCCCTTCTtccccc > 1:6266/1‑143 (MQ=255) aCGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCa > 1:625210/1‑143 (MQ=255) tccCTTATTGCTAATGATTGCCGGACTTCACATCTATCGGTTCCCAGCGGGCGACGTACGTAGAAAAAGCCGCCTTGCGGCAACGTGCGGTTTCTCGGCAGGTCCGCGTTACGTAAGGCGTCTTCTTCCTACGACGGTGTAtt > 2:774108/1‑142 (MQ=255) aTTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCAATTATTCCTTGAGCGGTTTATCAATGGc > 1:378006/1‑143 (MQ=255) tGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCgg < 1:1013959/143‑1 (MQ=255) tGCTAATGATTGCCGGACTCCACATCCGTGGTTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCgg < 1:336544/143‑1 (MQ=255) ggCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCgg < 2:707023/142‑1 (MQ=255) tactGATTGCCGGATTTCACATCCGTGGGTTGCCAGCGGGCAGCGTTCGTAGAAAAAACAGCCTTGCGGCAATGTGCGGTTTCCCGGCAGACCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGggga < 2:694672/140‑1 (MQ=255) tAATGATTGCCGGACTTCACATCCGTGTTTTCCCTACGGGTAACGTTCGTAGAAAAATCAGCGCTGCGACAACGAGCGCTTTCCCGGCAGATCCGTTTTACATAATGCTCAATATTCCTCCAGCGGTTTATCAATGGAgggtg > 2:473988/1‑141 (MQ=255) tAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGggga > 1:248396/1‑143 (MQ=255) tAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTGCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGCTTGTCGAGGGCggggg > 2:1038518/1‑142 (MQ=255) aagtaccgCCGGACTTCACACCCGGGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGgggag < 1:610373/136‑1 (MQ=255) aTGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTATTGCCTATTCTTCCTCCAGCGGTTTATCAATGGCGgggagg < 1:544648/143‑1 (MQ=255) ttGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAAt > 2:537244/1‑100 (MQ=255) ttGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAAt < 1:537244/100‑1 (MQ=255) tGCCGGACTTAACATCCGTGGGTGGCCAGCGGGTAACGAAAGAAAAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATc < 2:554253/143‑1 (MQ=255) gCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCAGCGACGGGCGGCTTCCCGGCAGATCCGTGTTACGTAATGCCCGCGAGTCCTCCAGCGGTGTGTGAACGGCGaggcggggagcc > 2:265872/1‑138 (MQ=255) cacaTCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCaataat > 1:587365/1‑143 (MQ=255) aTCCGTGGGTTGCCAGCGGGCAAGGTTCGTAGAAAAAACAGCCTTGCGGGAACGTGCGGCTCCACCGCACCCTTGGCTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAAGGGCGGGGAGGGAATCCAGCAATAATCgg < 2:571914/143‑1 (MQ=255) aacgTTGCCCGCTGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGgtgtg > 1:304126/4‑142 (MQ=255) aacgTTGCCCGCTGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTa > 1:452378/4‑143 (MQ=255) gggTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGAGCGGTGTCCCGGCAAATCCGCTGTACGGAACGCCCCGTCGGCCTCCAGCGCTTTCTCAATGGCGCGGAGCGAAGCCAGCAATACTCCGgcgtcg > 2:133300/1‑141 (MQ=255) acgTTGCCCGCTGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGCGGGAATCCAGCAATAATCGGGTGTAt > 1:568744/3‑143 (MQ=255) | AAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTAT > NC_000913/1557056‑1557328 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |