| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,674,958 | A→T | 7.4% | intergenic (+11/+14) | tqsA → / ← pntB | autoinducer 2 exporter/pyridine nucleotide transhydrogenase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,674,958 | 0 | A | T | 7.4% | 161.0 / 4.7 | 55 | intergenic (+11/+14) | tqsA/pntB | autoinducer 2 exporter/pyridine nucleotide transhydrogenase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (20/29); new base T (2/2); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01 | |||||||||||
GGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGAC > NC_000913/1674818‑1675097 | gggATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATAATTGTAAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCaga > 1:605510/1‑143 (MQ=255) gtgtGGTTGTGAGGTCCGGTGGCTAGTCTTCTTGCCTTGCCGTTGACAATTATGGTCAAAATTGCGCTTGAACACACAGCGGGAGTTCAAAGCAGCGCCGTGCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCagag < 2:796516/140‑1 (MQ=255) ggATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCagag < 1:15080/143‑1 (MQ=255) aTGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGCTCTGTTAAGCGATCTCAATAACGAGTGACGGCCTCCGCCGAGGc > 2:971262/1‑143 (MQ=255) ggTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCg < 2:835161/143‑1 (MQ=255) ggTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTGCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCg < 2:936323/143‑1 (MQ=255) gtggttCGGGCCGGTGGGTAGGCTGCTTGCCGTTCCGTTGGCAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAACCATCGCCGTGCGGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGt < 2:137795/140‑1 (MQ=255) gttAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGCGAGCCCGCCGCAGCACAGGCCGGCAg > 2:538474/1‑143 (MQ=255) aGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCCAGAAAGAGTGACGGGCTCAGCCGGGGCGGTCCGGGt > 1:752698/1‑143 (MQ=255) ggACCGGGGCGGATGCTGTTTTCCGTGTCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTACGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGtt < 1:242838/143‑1 (MQ=255) cccGGT‑GGTATGCTGCTCTCCGTGCCGTTGACAATTATTGTCAAAATTGCCCTTGAACAACCAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACa < 1:780281/142‑1 (MQ=255) gggTATGCTGCTTGCCGCGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAGGCATCGGCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCt < 2:174638/143‑1 (MQ=255) gTATGCTGCTTTCCGTGCCGTGGACAATTATTGTCAATATTGCGCTTGAACAACCAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCttt < 2:652250/143‑1 (MQ=255) tgctgcTTTCCGTGCCGTTGCCAATTATTGTCAAAATTGCGCTTGAACACACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGCGTGACGGCCTCAGCAGAGGACGTCAGGGTTACAGAGCTTTCaa > 1:11121/1‑142 (MQ=255) tgctgcTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGCGTGACGGCCTCAGCAGAGGCCGTCAGGGCGACAGAGCTGTCAg > 2:678059/1‑143 (MQ=255) tttCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGc < 1:230659/143‑1 (MQ=255) ttCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCGATAAAGAGTGACGGCCTCAGCAGCGGCCGTCAGGGTTACAGAGCTTGCAGGATAGct > 1:135102/1‑142 (MQ=255) cGTGCCGTTGACCATTATTGTCAAAATTGCGCTTGAGCACACAGCGTGAGGTCAAAGCATCGCCGTGCTGTGAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATcc < 2:11121/143‑1 (MQ=255) ccGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGc < 2:284320/143‑1 (MQ=255) aCAATTATTGTCAAAATTGCGGTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCtt < 2:611206/143‑1 (MQ=255) aCAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCtt > 1:998962/1‑143 (MQ=255) aaTTATTGTCAAAATTGTGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTg > 2:573164/1‑143 (MQ=255) aaTTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTAAATAAACAGTGCCGGCGTCAGCAGACGCCGTCAGGGTTACATAGCTTTCAAGATTGCATCCAAGCTGTCTTTg > 2:160941/1‑143 (MQ=255) attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGt < 1:368079/143‑1 (MQ=255) gTCAAAATTGCGCTCGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCAc < 1:864064/143‑1 (MQ=255) aaaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCaa > 1:744476/1‑143 (MQ=255) cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCCAAATAAAGAGTGACGGCCTCATCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGCCTTCGTCGTCACCAACCAGCacgc > 2:679873/1‑140 (MQ=255) ttGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAACTTTCAGGATTGCATACACGCTGGCTTTGGCGTCACCAACCAGCAtatg > 2:219763/1‑143 (MQ=255) caaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtga > 1:731183/1‑142 (MQ=255) aacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGttt > 1:107912/1‑143 (MQ=255) caGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTc < 1:779071/143‑1 (MQ=255) caGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTc < 1:787762/143‑1 (MQ=255) aGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAGGTGGGTGTTTTcc > 2:176562/1‑143 (MQ=255) gggTGGTCAGAGCATCGCCGTTCTGTGAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg < 2:446979/143‑1 (MQ=255) ggAGTTCAAAGCATCGCCGTGCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGGTTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga < 2:720336/143‑1 (MQ=255) ggAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga < 1:849271/143‑1 (MQ=255) ggAGGGCAAGGCGTGGTCGTGCGGTTAAGCGATCTCAATAAATAGTGACGGCCTCAGCAGACGGCGTCAGGGTGACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga < 2:777630/143‑1 (MQ=255) aaGCACCGCCGCTCGGTTAAGCGATCTCAAGAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGc < 2:1001392/143‑1 (MQ=255) aTCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAAGATTGCATCCACGCTGGCTTTGGCGGCACCAAACAGCATGCGGGTGGCTCCCATGAAGACCAGCgggc > 2:450595/1‑142 (MQ=255) cGCCGTGCTGTTGAGCGATGTCGATAAAGAGTGACGGCCTCAGCAGAGGCCGGCAGGGTTACAGAGCTTTCAGGATGGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGttt < 2:266917/143‑1 (MQ=255) gCCGTTCTGGTAAGCGATCTCAAGAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGtttt < 1:868525/143‑1 (MQ=255) tCTGTTGAGCGAGGTCGATAGAGAGTGACGGCCTCAGCAGAGGCCGGCAGGGTTACAGAGCTTTCAGGATTGCATTCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGcaca < 2:316893/143‑1 (MQ=255) cTGTTAAGCGATCGCAATAAAGAGGGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCacac < 1:733604/143‑1 (MQ=255) tacgCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGc < 1:871727/140‑1 (MQ=255) tAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGc < 1:104159/143‑1 (MQ=255) aGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCAt > 1:963657/1‑143 (MQ=255) aGCGATCTAAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTGCTTGACGAACAGCGGGTTTTGCACACCAGCAt > 2:241548/1‑143 (MQ=255) cGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGCTTTCCTTGAAGAACAGCGGGTTTTGCACCCCCGCATAg > 1:291863/1‑143 (MQ=255) aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGcc < 1:388650/143‑1 (MQ=255) tcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAgt > 1:449111/1‑143 (MQ=255) aaGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTAGCGTCACCATACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCAt > 2:854788/1‑143 (MQ=255) gTGACGGCCGCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGaa < 1:492885/143‑1 (MQ=255) tGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAAc > 1:923034/1‑143 (MQ=255) aCGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCGGTGTTCATCGAACGt > 1:915107/1‑143 (MQ=255) aCGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGt < 1:573164/143‑1 (MQ=255) gCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTTAAGAACAGCGGGTTTTGCACACCAGCATAGCCATTGTTCATCGAACGTTTa > 1:276626/1‑143 (MQ=255) ccgcagcagAGGCCGTCAGGGTTGCAGAGCTTTAAGGATTGCATTCCCGCTGGCTTTGGCGTCTCCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTaa < 2:566287/140‑1 (MQ=255) cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTaaa < 2:449111/143‑1 (MQ=255) cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTaaa < 1:75471/143‑1 (MQ=255) agcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTCTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGAc > 1:830246/1‑143 (MQ=255) | GGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGAC > NC_000913/1674818‑1675097 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |