Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,948,179 T→A 6.6% intergenic (‑28/+1) yebC ← / ← nudB putative transcriptional regulator YebC/dihydroneopterin triphosphate diphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,948,1790TA 6.6% 165.2 / 4.3 61intergenic (‑28/+1)yebC/nudBputative transcriptional regulator YebC/dihydroneopterin triphosphate diphosphatase
Reads supporting (aligned to +/- strand):  ref base T (29/28);  new base A (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.73e-02

CAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAG  >  NC_000913/1948038‑1948320
                                                                                                                                             |                                                                                                                                             
cAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATCAAAATATCTCCAGATAGCCCTGCCTGtt                                                                                                                                              >  1:694340/1‑143 (MQ=255)
     gaGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGc                                                                                                                                         <  2:409197/142‑1 (MQ=255)
        ccGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGc                                                                                                                                      >  1:648292/1‑143 (MQ=255)
        ccGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGAACAGGCAgg                                                                                                                                      <  2:895978/143‑2 (MQ=255)
        ccGCGGTGACCAGCTCACGAGTGAGTGTATTGGAGATTTTACCGCGCTTAGCATCCTGCGCAGGTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGAACAGGCAgg                                                                                                                                      <  2:964023/143‑2 (MQ=255)
          gcgGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCCTAAAAATATCTCCAGATAGCCCTGCCGGTTCAGGCAGCGt                                                                                                                                    >  2:96339/1‑143 (MQ=255)
          gccgctACCAGCTCACGACTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGt                                                                                                                                    <  1:177062/138‑1 (MQ=255)
              tgacgaGCTCACGAATGGTTTGAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCTCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAAt                                                                                                                                <  2:151163/138‑1 (MQ=255)
               tACCAGCTCCCGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAAtt                                                                                                                               <  2:875622/143‑1 (MQ=255)
               tACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAAtt                                                                                                                               >  2:634451/1‑143 (MQ=255)
                  cAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGTGTTACTTACa                                                                                                                            >  2:651506/1‑143 (MQ=255)
                  cAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACa                                                                                                                            >  1:730606/1‑143 (MQ=255)
                   aGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTTGCCCATTTACTATGACCTGCCATAACAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACaa                                                                                                                           >  2:39462/1‑143 (MQ=255)
                    gctgtcGAATGATTTTAGTGAAGGTTTGACCGCGCTTCGCATTCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACaga                                                                                                                          <  2:942729/138‑3 (MQ=255)
                    gCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGGTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACaaa                                                                                                                          <  1:189717/143‑1 (MQ=255)
                       cACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTg                                                                                                                       <  1:571030/143‑1 (MQ=255)
                       cACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAAATg                                                                                                                       >  1:135070/1‑143 (MQ=255)
                          gAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGCTCGCCCTGCCTGTTCAGGCCGCGCTAATTACAAACTGTTc                                                                                                                    >  1:419532/1‑143 (MQ=255)
                          gAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGAGTTCGTTACAAAGTGTTc                                                                                                                    >  2:779647/1‑143 (MQ=255)
                           aaTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCa                                                                                                                   <  1:513893/143‑1 (MQ=255)
                           aaTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGAGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCAAGATAGCCCTGCCTGTTCGGGCAGCGTTAATTACAAGCTGTTCa                                                                                                                   >  1:317419/1‑143 (MQ=255)
                            aTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCaa                                                                                                                  <  2:346823/143‑1 (MQ=255)
                              gATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTTCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATc                                                                                                                >  2:627002/1‑143 (MQ=255)
                                 ttttGTGATGATTTTAGCGCTCTGATCATCCTGCGCAGCTTTACGATGTCTTATCTTTACAATTTTCCTACAACCTGCCATAAAAATATCTCCAGATAGCCCCGCGTGTTCAGGCAGCGTTAATTACAAACTGTTCAATcccc                                                                                                             <  2:122347/143‑1 (MQ=255)
                                  ttAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCt                                                                                                            >  2:47696/1‑143 (MQ=255)
                                      tGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCg                                                                                                        <  1:623130/143‑1 (MQ=255)
                                          gATTTCACCGCGCTTAGCATCCTGCGCAGCTTTTCGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGTCGGTTg                                                                                                    <  1:260830/143‑1 (MQ=255)
                                                  cgcgCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGa                                                                                            >  2:818367/1‑143 (MQ=255)
                                                  cgcgCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGa                                                                                            >  2:477312/1‑143 (MQ=255)
                                                       ttAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGAGTGGATTACAAACTGTTCAATCGGCTGACGGGTGACCTAGGACTGAg                                                                                       >  2:459608/1‑143 (MQ=255)
                                                           cATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAg                                                                                   >  1:954153/1‑143 (MQ=255)
                                                           cATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAg                                                                                   >  1:325100/1‑143 (MQ=255)
                                                             tCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAgcg                                                                                 >  2:375830/1‑143 (MQ=255)
                                                                 gcgcAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGcgccgc                                                                             <  1:844185/143‑1 (MQ=255)
                                                                          tACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAgg                                                                    <  2:69706/143‑1 (MQ=255)
                                                                           aCGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGgc                                                                   >  2:697367/1‑143 (MQ=255)
                                                                                   tGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGCTTGCTCCAGGACTTAGGGCGCGCCGCCGCAGCAGGCGCATCAAg                                                           >  1:261047/1‑143 (MQ=255)
                                                                                    cgggtcggCCCATTTACTATGACCTGCCCTAACAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACACACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGc                                                          <  2:489786/137‑1 (MQ=255)
                                                                                       gtTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCAc                                                       <  1:209783/143‑1 (MQ=255)
                                                                                           gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCACGCCACTTGt                                                   >  1:599414/1‑143 (MQ=255)
                                                                                           gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAACCCCCTTGt                                                   >  1:86550/1‑143 (MQ=255)
                                                                                           gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCAGCCGCAGCAGGCGCATCGAGCCACTTGt                                                   >  2:307825/1‑143 (MQ=255)
                                                                                            cccAGTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCCGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTa                                                  <  2:674782/143‑1 (MQ=255)
                                                                                             ccATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTaa                                                 <  1:627002/143‑1 (MQ=255)
                                                                                             ccATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCCGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTaa                                                 <  2:694340/143‑1 (MQ=255)
                                                                                                          ccTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAg                                    <  2:828047/143‑1 (MQ=255)
                                                                                                           cTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGt                                   >  1:781140/1‑143 (MQ=255)
                                                                                                            tGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTg                                  <  1:634451/143‑1 (MQ=255)
                                                                                                                       tatCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTg                       <  1:834378/143‑1 (MQ=255)
                                                                                                                       tatCGCCAGATAGCCCTGCCCGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTg                       <  2:336165/143‑1 (MQ=255)
                                                                                                                         tctcCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGCAAGCCAGGTGTTCAGTGAACACGATCTGcc                     >  2:777529/1‑143 (MQ=255)
                                                                                                                           tcCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGCTGTTCAGTGAAAACGATCTGCCGc                   >  2:403529/1‑143 (MQ=255)
                                                                                                                           tcCAGATAGCCCTGCCTGTTCAGGCAGCGTGAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTGAGTGAGCGCCGCCGCAGCCGGCGCAGCAAGCCACTTGTAAGCCAGATGTTCAGTGAAACCGATCCCCCGc                   >  2:283790/1‑143 (MQ=255)
                                                                                                                                aTAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAAATGTTCAATCGCCTGCCGGTTGCTCCAGGACTTCGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTg              >  1:800475/1‑143 (MQ=255)
                                                                                                                                 tAGCTCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGc             <  2:1007740/143‑1 (MQ=255)
                                                                                                                                 tAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGc             <  1:818367/143‑1 (MQ=255)
                                                                                                                                  cgCCCTGCCTGTTCAGGCAGCGTTAATTACTAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCg            <  2:335877/142‑1 (MQ=255)
                                                                                                                                   acgctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCgg           >  1:631798/4‑143 (MQ=255)
                                                                                                                                    cgctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGa          >  2:1038877/3‑143 (MQ=255)
                                                                                                                                      ctgcctgTGCAGGCAGCGTTAATTACAAACTGTTCAAGCGCCTGCCGGTGGCTCCAGGACTGAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGGGAAAACGATCTGCCGCTCGTGCGGAAg        <  1:419963/143‑1 (MQ=255)
                                                                                                                                       tgcctgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAgc       <  1:375830/143‑1 (MQ=255)
                                                                                                                                            gTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCaac  >  1:757483/1‑142 (MQ=255)
                                                                                                                                             |                                                                                                                                             
CAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAG  >  NC_000913/1948038‑1948320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑